Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 7 of 7 for “"Fluorescence polarization assay"”.
-
Presumptive identification of smooth Brucella strain antibodies in canines
… We hypothesize that the Brucella abortus Fluorescence Polarization Assay would be successful in identifying antibodies to smooth Brucella strain in canines. Ninety-five dogs, including forty-five hog hunting dogs were screened for circulating antibodies to any of the four zoonotic strains …
-
Mechanistic studies to evaluate the targeting specificity of novel RGD Micelles to the αVβ3 integrin receptor
… integrin through a competitive binding fluorescence polarization assay (with novel RGD micelles displacing an integrin-bound fluorescent RGD probe by as much as 63.03%). Ultimately, these nanocarriers were assessed for their ability to deliver phys ically entrapped fluorescein isoth …
-
Targeting Lsr2/DNA Complexation for Dysregulation of Gene Expression in <i>Mycobacterium tuberculosis</i>
… describes the development of a high-throughput assay that enabled the identification of Zafirlukast as an inhibitor of the Lsr2/DNA complexation. This ability was shown in vitro using a fluorescence polarization assay. Zafirlukast also exhibits growth inhibition of <i>Mycobacterium smegmatis</i> …
-
Chimera Ligand for Pili and Lectin A Protein Controls Antibiotic-Promoted Biofilm Formation, Swarming Motility, Tolerance and Persister Formation by Pseudomonas aeruginosa
… chimera ligands was demonstrated by competitive fluorescence polarization assay (LecA) and by adding a functional group to a ligand that can covalently attach to the receptor protein only when the physical ligand-receptor binding takes place (Pili). In addition, the effect of externally added …
-
Mechanistic studies of class II lanthipeptide synthetases and yeast surface display of lanthipeptide leader peptides
… three consistent aspartate mutations. Using fluorescence polarization assay, it was confirmed that the binding affinity of the mutant leader peptide was about 5-fold stronger than the WT leader peptide. Furthermore, LctA containing the engineered leader peptide was shown to be a more …
-
Nuclear Export Signal Recognition by CRM1 Carrying the Oncogenic E571K Mutation and Structure-Based NES Prediction
… a low-throughput differential photobleaching assay. However, hundreds of very diverse CRM1 cargos and their NESs have yet to be characterized, limiting our understanding of the biological roles of CRM1 in disease. I developed a high-throughput, quantitative fluorescence polarization assay to …