Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 490 for “"Fluorescence microscopy."”.
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Single molecule fluorescence microscopy of carbon nanotubes
Single molecule microscopy has been extensively used in the past decade to study individual biomolecules with excellent spatial and temporal resolution. Meanwhile, characterization of nanomaterials and their development towards a variety of biological applications has progressed rapidly. Despite …
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Super-Resolution Fluorescence Microscopy using Transparent Waveguide Chips
Super-resolution fluorescence microscopy (optical nanoscopy) allows us to surpass the optical diffraction limit and observe biological phenomena at the nanoscale using visible light. Different modalities come with their own trade-offs, such as imaging speed vs. achieved resolution or live cell …
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Three-Dimensional Fluorescence Microscopy by Optical Scanning Holography
As three-dimensional (3D) imaging and fluorescence techniques become standard in optical microscopy, novel approaches to 3D fluorescence microscopy are emerging. One such approach is based on the incoherent holography technique called optical scanning holography (OSH). The main advantage of …
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Advancing Fluorescence Microscopy Techniques for Volumetric Whole-cell Imaging
… advancing volumetric whole-cell imaging based on fluorescence microscopy. The first project establishes a robust single-molecule localisation microscopy (SMLM) pipeline for volumetric imaging in the whole cell using the double-helix point spread function (DH-PSF) approach. The practical challenges …
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Volumetric Imaging Across Spatiotemporal Scales in Biology with Fluorescence Microscopy
… questions in modern biological research. Fluorescence microscopy has emerged as an indispensable tool in generating such maps, but common techniques are limited by fundamental physical constraints which render them incapable of simultaneously achieving high spatial and temporal resolution. …
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Automated Tracking of Mouse Embryogenesis from Large-scale Fluorescence Microscopy Data
Recent breakthroughs in microscopy techniques and fluorescence probes enable the recording of mouse embryogenesis at the cellular level for days, easily generating terabyte-level 3D time-lapse data. Since millions of cells are involved, this information-rich data brings a natural demand for an …
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Catalytic Studies Of Noble Metal Nanocatalysts Using Single-Molecule Fluorescence Microscopy
… to quantify these differences. A highthroughput fluorescence microscopy technique was developed in which a nonfluorescent reactant gets catalyzed to a fluorescent product on a gold catalyst surface. By recording the fluorescence signals at the single-molecule level from many particles on a camera …
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Next-generation fluorophores for single-molecule and super-resolution fluorescence microscopy
… of single-molecule and super-resolution fluorescence techniques has revolutionised biological imaging. Nano-scale cellular structures and heterogeneous dynamic processes are now able to be visualised with unprecedented resolution in both time and space. The achievable localisation …
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Computational image analysis of subcellular dynamics in time-lapse fluorescence microscopy
The use of image segmentation and motion tracking algorithms was adapted for analyzing time-lapse data of cells with fluorescently labeled protein. Performance metrics were devised and algorithm parameters were matched to hand-created ground-truth data. The performance of these algorithms in this …
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Automated Fluorescence Microscopy Determination of Mycobacterium Tuberculosis Count via Vessel Filtering
Tuberculosis (TB), a deadly infectious disease caused by the bacillus Mycobacterium tuberculosis (MTB), is the leading infectious disease killer globally, ranking in the top 10 overall causes of death despite being curable with a timely diagnosis and the correct treatment [3]. As such, eradicating …
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2D timelapse and 3D fluorescence microscopy with applications to vascular tissue engineering
… cells (EC). Two dimensional timelapse fluorescence microscopy revealed rapid alkalinization occurring in cultured human aortic SMC exposed to well defined fluid flow profiles. The response was reversible and persisted for at least 20 min after flow initiation. The magnitude of the …
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Using Advanced Fluorescence Microscopy to Analyze Intracellular Trafficking of FcRn In Live Cells
The file named "GanZhou.pdf" is the primary dissertation file. All other files ("*.mov") are supplemental files and may be viewed individually.
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Examining the Role of Phospholamban Phosphorylation on Interaction with SERCA Using Fluorescence Microscopy
… SERCA and PLB respectively, in HEK293 cells for fluorescence resonance energy transfer (FRET) microscopy experiments. We were able to use the native beta-adrenergic signaling system in the cells to control the state of PLB phosphorylation in a time-dependent manner. For the dissociation model to …
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Protein segregation and conformation in antigen-receptor triggering: a quantitative fluorescence microscopy study
… triggering. Total internal reflection fluorescence microscopy is used to establish that phosphatase exclusion decreases when intermembrane distance increases. This inverse relationship, demonstrated for both B cells and T cells, is consistent with the kinetic-segregation model. However, …
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Computing traction forces, intracellular prestress, and intracellular modulus distribution from fluorescence microscopy image stacks
Cell modulus and prestress are important determinants of cell behavior. This study creates new software tools to compute the modulus and prestress distribution within a living cell. As input, we have a sequence of images of a cell plated on a substrate with fluorescently labeled fibronectin dots. …
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Direct visualization of electron transport among nonconjugated polymeric redox-active colloids via fluorescence microscopy
… to developing a new imaging methodology based on fluorescence microscopy, to directly visualize electron transport processes among micron-sized redox active colloids (RAC) containing high densities of ethyl-viologen (EV) pendant groups. This observation was enabled by the discovery that these RAC …
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Visualization of energy transfer among redox active polymeric colloids via in-situ fluorescence microscopy
… showed a considerably increased sensitivity to fluorescence quenching. Investigation of the amplified sensitivity was done and this was attributed to fast electron hopping among neighboring redox groups.
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Applications of Pump-Probe and Two-Photon Techniques in Time-Resolved Fluorescence Microscopy and Spectroscopy
Two-photon fluorescence microscopy has proven to be an useful technique for imaging biological systems. Good spatial resolution, reduced photobleaching, and efficient rejection of excitation wavelength from emitted fluorescence are the main characteristics of this technique. As part of this work, …
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