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Showing 1 to 17 of 17 for “"Fluorescence methods"”.
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Polymer dynamics and fluctuations probed with fluorescence methods
… of polymer chain properties with potent fluorescence methods imported from other interdisciplinary sciences and applied successfully to polymer physics problems for the first time. The focus is an important theme in equilibrium polymer physics - chain structure and dynamics at …
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A Versatile Tunable Dye Laser for Spectrochemical Fluorescence Methods
Made available in DSpace on 2014-12-13T20:09:36Z (GMT). No. of bitstreams: 1 7606780.pdf: 5139510 bytes, checksum: b1ceecc905469cf55f952792ab87c240 (MD5) Previous issue date: 1975
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Detection of vapor phase mercury species by laser fluorescence methods
… from industrial stacks. Conventional measurement methods, such as cold vapor trap based techniques for elemental mercury, have difficulty in achieving both high sensitivity and the fast time resolution required for real-time monitoring. This doctoral research work gives a systematic study of …
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DEVELOPMENT OF KRYPTON PLANAR LASER INDUCED FLUORESCENCE METHODS FOR THE MEASUREMENT OF HYPERSONIC FLOW CONDITIONS
… which come in contact with the flow. Intrusive methods become impractical for high Mach number flows, as such methods can cause considerable disruption to the integrity of flow measurements. Therefore, it is desirable to utilize non-intrusive methods in such experiments, especially as hypersonic …
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The Further Characterization of <i>Saccharomyces cerevisiae</i> Ribosomal Protein L30 and Its Interaction with the <i>RPL30</i> Transcript through Mutational Analysis, Circular Dichroism, and Fluorescence Methods
… alternative to the filter binding experiments, a fluorescence binding assay was developed and implemented. When the RNA binds and brings the nucleotides close to a fluorescently labeled cysteine, the fluorescence of the fluorophore is quenched by the purine-rich RNA internal loop. Through <em>in …
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A study of the binding of anti-aggregatory substances to platelet bound plasma proteins.
… serum albumin was studied using several direct fluorescence methods and fluorescence polarisation. Particular emphasis was placed on the study of the binding of dipyridamole (DPD) and its analogues. The data obtained from the above experiments was compared with that obtained from the classical …
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Sample and counting variations associated with x-ray flourescence [sic] analysis
X-ray fluorescence methods offer the chemist a much speedier analysis of multicomponent mixtures than conventional procedures. Components can be analyzed by measuring the intensity of emission lines sent out after rays strike the mixture, However, variations in the measurement of intensity do occur …
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Studies of bronze electrodeposition.
… chemically by atomic absorption and X-ray fluorescence methods and studied structurally by X-ray diffraction. By application of the experimental data to the optimum plating conditions already specified a rudimentary mass balance for the system has been calculated. The possibility of …
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Development of an Enzyme-Linked ImmunoSorbent Assay (ELISA) with Enhanced Sensitivity in a Nanofluidic System
… nanochannel were detected using electrical and fluorescence methods. We concluded that antibody-antigen binding constants in nanochannels were similar to experiments performed in microtiter plates at low antigen concentrations; however the bound fraction in nanochannels at high antigen …
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Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine
… of mutagenesis, disulfide crosslinking, and fluorescence methods to assay the conformations and nucleotide-binding properties of individual subunits, we demonstrate dynamic interconversion of loadable and unloadable subunits. Such interconversion is required to couple ATP hydrolysis by ClpX …
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Role of Cofilin, an Actin Cytoskeletal Protein, in Ischemic Conditions: Potential Therapeutic Target for Ischemic Stroke
… expression profile studies by immunoblotting and fluorescence methods displayed decrease in phosphocofilin (inactive form of cofilin) levels with all models of ischemia implying stress induced cofilin activation. Furthermore, calcineurin and slingshot 1L (SSH) phosphatases were found to be the …
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New tools derived from the solvatochromic 4-N,N-dimethylamino-1,8-naphthalimide fluorophore for the detection of biomolecular interactions
… at the cellular level, new tools are required. Fluorescence methods have formed a cornerstone of modern cell biology and have allowed researchers to interrogate the dynamics of many biomolecular events in real-time. However, the criteria for a good fluorescent probe are often numerous and …
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Microfabricated magnetophoretic focusing systems for the separation of submicrometer particles
… were tested via experiments using optical fluorescence methods to observe the concentration profiles of polydisperse suspensions of polystyrene beads.
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Passive transport through biological membranes
… for biological systems. In Chapter 2 I use fluorescence methods to determine the passive transport of molecules with self intrinsic fluorescence through lipid membranes. We have built a unique fluorescence microscope which is capable of visualizing the fluorescence of molecules excited with …
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Investigation of the dynamics and conformational changes of the U1A protein
… RNA complex using laser T-jump and stopped-flow fluorescence methods. Analysis of the kinetic data suggested three phases of dissociation with two intermediate states. Chapter 4 is a follow up study of chapters 2 and 3 to further investigate helix C dynamics. The experimental design makes use of …
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Studies of CD36 interacting with fatty acids, oxidized low-density lipoprotein, and the cellular plasma membrane
… CD36 were studied by cell biological and fluorescence methods. To test our hypothesis that the PM is not an impermeable barrier to FA and that FA move into cells by diffusion via their uncharged form, we first applied biophysical fluorescence spectroscopy to directly measure transmembrane …
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Monitoring of Persistent Organic Pollutants (POPs) in marine biota from the Catania Gulf (Sicily, Italy) and development of new sensitive analytical methods for their detection
… mass spectrometry (GC-MS) methodologies and the fluorescence spectroscopy, to assess the distribution of different classes of Persistent Organic Pollutants (POPs), including polychlorinated biphenyls (PCBs), organochlorine pesticides (POCs), and polycyclic aromatic hydrocarbons (PAHs), in …