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Showing 1 to 20 of 30 for “"Fluorescence correlation spectroscopy (FCS)"”.
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Interplay of Polyelectrolytes With Different Adsorbing Surfaces
… with polyelectrolytes are presented, using fluorescence correlation spectroscopy (FCS). The significance is to characterize domains induced by polyelectrolyte binding.
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Understanding colloidal quantum dot excitation with solution photon correlation fourier spectroscopy
… exist in inhomogenous batches. Solution photon correlation fourier spectroscopy (S-PCFS) combines interferometry with fluorescence correlation spectroscopy (FCS) to measure the spectral correlation of the average single fluorophore in a solution. With S-PCFS, the intrinsic emission linewidth of …
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Interactions and Dynamics of Soft Materials
… impact on the advanced technology. We borrowed Fluorescence Correlation Spectroscopy (FCS) from biophysics field to investigate thee molecular weight dependence, surface coverage dependence, surface property dependence of polymer interfacial diffusion. We also developed a set of novel …
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Investigating lipid corona formation onto polystyrene nanoparticles through fluorescence correlation spectroscopy
… which is composed of phospholipid bilayer. Fluorescence correlation spectroscopy (FCS) is a promising tool that can be used to probe nanoparticle-cell interactions. FCS experiments focusing on exposure of lipid vesicles to different polystyrene nanoparticles indicate possible formation of a …
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Fluorescence Depletion by Stimulated Emission in Single-Molecule Spectroscopy
… (STED) technique to the field of single-molecule fluorescence spectroscopy. It is demonstrated that fluorescence quenching induced by STED is reversible and can be repeated a large number of cycles on a single molecule. Being ideal point-like probes, single molecules can therefore be used to …
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Investigation of peptide-lipid interaction by fluorescence correlation spectroscopy
… lipid model membranes was investigated using fluorescence correlation spectroscopy (FCS) and laser scanning confocal imaging. A similar mechanism of MV4s was observed as compared to V4. MV4s induced lipid aggregation before they disrupted the lipid membranes. By comparing between different …
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A Fluorescence Correlation Spectroscopy Study of the Cryoprotective Mechanism of Glucose on Hemocyanin
… In order to test this hypothesis, two-photon fluorescence correlation spectroscopy (FCS) was used to measure the hydrodynamic radius of hemocyanin (Hc), an oxygen-carrying protein found in arthropods, in glucose solutions up to 20wt%. FCS found that the hydrodynamic radius was invariant with …
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Protein Dynamics: Studies of Adenylate Kinase Mutants From Escherichia Coli and Characterization of Adenylate Kinase Isoforms From Murine Cells. Applications of Fluorescence Spectroscopy and Microscopy
… & AK1beta) from murine cells using a series of fluorescence microscopic techniques (two-photon excitation fluorescence imaging, fluorescence correlation spectroscopy (FCS), fluorescence lifetime imaging (FLIM)). In the in vivo studies, the genes of AK1 and AK1beta were fused with the gene of …
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Investigating the mechanotransduction by two-photon fluorescence microscopy measurement of intracellular free energy of binding
… and FAT-mCherry, using two-photon excitation fluorescence microscopy and the utility of it as a measure of protein conformational change. The hypothesis tested is that force-induced changes in protein conformation alter inter-protein binding affinity. A comprehensive toolkit that utilizes …
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Fluorescence fluctuation spectroscopy and microscopy : application to cellular molecular dynamics and organization
Fluorescence fluctuation spectroscopy and microscopy have been powerful tools for studying molecular organization and dynamics in cells. Fluorescence Correlation Spectroscopy (FCS) has been widely applied to probing molecular dynamics in live cells with single molecule sensitivity and the ability …
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Analyse und Visualisierung eines R2R3-MYB / bHLH Transkriptionsfaktorkomplexes aus Arabidopsis thaliana
… analyzed with new methods from the physics like fluorescence correlation spectroscopy (FCS) or fluorescence resonance energy transfer (FRET). With FCS and recombinant expressed proteins, promoter attachment sites of the transcription factor complex were identified. Simultaneous binding of both …
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The ATP synthase from spinach chloroplasts (Spinacea oleracea) : improved isolation and purification of the enzyme and conformational changes observed with fluorescent labels
… which can be investigated with the help of Fluorescence Resonance Energy Transfer (FRET), Fluorescence Lifetimes (FL) or with Fluorescence Correlation Spectroscopy (FCS). <br>Fluorescent probes were used to selectively label different subunits of CF1, in order to conduct spectroscopic …
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Vibrational Spectroscopy of Self-Forming Synthetic PEGylated Lipids and Nanovesicles
Vibrational spectroscopy has been used to elucidate the structure and conformation of lipids and nanovesicles. In this study, three different vibrational spectroscopic techniques, namely near-infrared (NIR), Fourier transform infrared (FTIR) and Raman spectroscopies, have been employed for the …
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Dynamics of DNA Breathing and Folding for Molecular Recognition and Computation
… and quencher are held in close proximity and the fluorescence is quenched; when the molecular beacon is open, fluorophore and quencher are far apart, and the fluorescence is restored. Molecular beacons are ideal DNA probes coupling conformational switch with fluorescence signal turning-ON. We use …
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Examining Post-Translational Modifications In Alpha-Synuclein Through Semi-Synthesis And Single Molecule Spectroscopy
… we examine the function of modified αS using fluorescence correlation spectroscopy (FCS) to assay its affinity for lipid vesicles. We find that neither phosphorylation nor arginylation alters lipid binding. However, we observe by smFRET that phosphorylation gives rise to conformational …
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DESIGNING BIOMIMETIC NANOPARTICLES TO TACKLE INTRACELLULAR PATHOGENS
… protein corona formation was detected using fluorescence correlation spectroscopy (FCS), suggesting desired biomimetic characteristics. Lectin staining confirmed plasma membrane origin and preservation of surface glycocalyx profiles. Partial retention of surface markers like CD14 (CD14+ …
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Advanced Fluorescence Correlation Techniques to Study Membrane Dynamics
Fluorescence Correlation Spectroscopy (FCS) is a powerful tool to measure important physical quantities such as concentrations, diffusion coefficients, diffusion modes or binding parameters, both in solution and in membranes. However, it can suffer from severe artifacts, especially in non-ideal …
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Optical instruments for characterization in the nano-scale
… thesis two such instruments, the Florescence Correlation Spectroscope for determination of hydrodynamic size of quantum dot bioprobes to nanometer precision in part one, and the Long Trace Profiler/Optical Slope Measurement System for characterization of line spacing errors in X-ray blazed …
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Synthesis, Monitoring, and Molechanical Action of Light-Driven Motorized Nanomachines
… (2 rph at 60 °C), we found using single molecule fluorescence correlation spectroscopy (FCS) that only the molecules with fast rotating speed (MHz range) show an enhancement in average diffusion by 26% when the motor is fully activated by UV light. A non-unidirectional rotating motor also results …
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Single-Molecule Characterization of Photophysical and Colloidal Properties of Biocompatible Quantum Dots
… need to be thoroughly monitored. Single-molecule fluorescence detection was used to characterize NC fluorescence, observe intermittency in the emission (blinking), and unravel a non-fluorescent subpopulation of NCs whose fluorescence can be restored through photo-induced activation. Stage-scanning …
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