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Showing 1 to 20 of 53 for “"Fluorescence correlation spectroscopy"”.
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Silicon Nanoparticle Characterization by Fluorescence Correlation Spectroscopy
Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Elizabeth Kent (eckent2@illinois.edu) on 2012-05-31T16:14:05Z Item is restricted indefinitely.
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New Applications of Fluorescence Correlation Spectroscopy in Materials Science
… we have developed new concepts for the usage of fluorescence correlation spectroscopy. A classical FCS setup was modified in such a way that fluorescent species in aqueous as well as organic environments can be studied at varying temperature. We have synthesised a set of dye-labelled polymers …
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Investigation of peptide-lipid interaction by fluorescence correlation spectroscopy
… lipid model membranes was investigated using fluorescence correlation spectroscopy (FCS) and laser scanning confocal imaging. A similar mechanism of MV4s was observed as compared to V4. MV4s induced lipid aggregation before they disrupted the lipid membranes. By comparing between different …
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Fluorescence correlation spectroscopy: ultrasensitive detection in clear and turbid media
… bright particles). The data is analyzed with a correlation filter program based on particle passage pattern recognition. I will also describe further work on improving the sensitivity of the technique, expanding it for multiple-species discrimination and enumeration, and testing the prototype …
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Investigating lipid corona formation onto polystyrene nanoparticles through fluorescence correlation spectroscopy
… which is composed of phospholipid bilayer. Fluorescence correlation spectroscopy (FCS) is a promising tool that can be used to probe nanoparticle-cell interactions. FCS experiments focusing on exposure of lipid vesicles to different polystyrene nanoparticles indicate possible formation of a …
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Investigating transport of charged nanoparticles by multiphoton fluorescence correlation spectroscopy (MPFCS)
… describes the development of a multiphoton fluorescence correlation spectroscopy (MPFCS) system and its utilization to probe the effect of charge on the diffusion of quantum dot (QD) nanocrystals in collagen and collagen-hyaluronan (HA) composite gel models of human colon adenocarcinoma …
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A novel integrated platform combining atomic force microscopy and fluorescence correlation spectroscopy
… liquids. Atomic force microscope (AFM) and fluorescence correlation spectroscope (FCS) are two complimentary techniques that are commonly employed investigate mechanics and dynamics of liquids at interfaces. In this thesis, the design and construction of a novel integrated platform combining …
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A Fluorescence Correlation Spectroscopy Study of the Cryoprotective Mechanism of Glucose on Hemocyanin
… In order to test this hypothesis, two-photon fluorescence correlation spectroscopy (FCS) was used to measure the hydrodynamic radius of hemocyanin (Hc), an oxygen-carrying protein found in arthropods, in glucose solutions up to 20wt%. FCS found that the hydrodynamic radius was invariant with …
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Single-particle tracking and fluorescence correlation spectroscopy for systems-level analysis of molecular dynamics in diverse biological systems
Fluorescence microscopy has proven to be immensely powerful for the study of biological systems at both the cellular and systems biological levels. The ability to specifically label a single molecular species fluorescently has enabled the study of complex cellular structures through the …
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Interplay of Polyelectrolytes With Different Adsorbing Surfaces
… with polyelectrolytes are presented, using fluorescence correlation spectroscopy (FCS). The significance is to characterize domains induced by polyelectrolyte binding.
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Structure and Dynamics of the Myosin Dimer Using Fluorescence Techniques
… called two-color FRET-FCS (a combination of Fluorescence Resonance Energy Transfer and Fluorescence Correlation Spectroscopy) and its application to the dynamical study of a model DNA hairpin. This technique can ultimately become useful in probing protein dynamics.
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Understanding colloidal quantum dot excitation with solution photon correlation fourier spectroscopy
… exist in inhomogenous batches. Solution photon correlation fourier spectroscopy (S-PCFS) combines interferometry with fluorescence correlation spectroscopy (FCS) to measure the spectral correlation of the average single fluorophore in a solution. With S-PCFS, the intrinsic emission linewidth of …
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Interactions and Dynamics of Soft Materials
… impact on the advanced technology. We borrowed Fluorescence Correlation Spectroscopy (FCS) from biophysics field to investigate thee molecular weight dependence, surface coverage dependence, surface property dependence of polymer interfacial diffusion. We also developed a set of novel …
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Fluorescence Depletion by Stimulated Emission in Single-Molecule Spectroscopy
… (STED) technique to the field of single-molecule fluorescence spectroscopy. It is demonstrated that fluorescence quenching induced by STED is reversible and can be repeated a large number of cycles on a single molecule. Being ideal point-like probes, single molecules can therefore be used to …
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New analytical scenarios and new approaches in the embryonic genetic investigation of the macromolecular alterations responsible for the neurodegenerative diseases
… correlazione di fluorescenza risolta nel tempo, Fluorescence Correlation Spectroscopy (F.C.S.) con particolare riferimento alla tecnica di Number of Molecules and Brightness (N&B), una applicazione innovativa delle tecniche di imaging molecolare. Questo tipo di analisi ha consentito di monitorare …
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Protein Dynamics: Studies of Adenylate Kinase Mutants From Escherichia Coli and Characterization of Adenylate Kinase Isoforms From Murine Cells. Applications of Fluorescence Spectroscopy and Microscopy
… & AK1beta) from murine cells using a series of fluorescence microscopic techniques (two-photon excitation fluorescence imaging, fluorescence correlation spectroscopy (FCS), fluorescence lifetime imaging (FLIM)). In the in vivo studies, the genes of AK1 and AK1beta were fused with the gene of …
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Investigating the mechanotransduction by two-photon fluorescence microscopy measurement of intracellular free energy of binding
… and FAT-mCherry, using two-photon excitation fluorescence microscopy and the utility of it as a measure of protein conformational change. The hypothesis tested is that force-induced changes in protein conformation alter inter-protein binding affinity. A comprehensive toolkit that utilizes …
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Fluorescence fluctuation spectroscopy and microscopy : application to cellular molecular dynamics and organization
Fluorescence fluctuation spectroscopy and microscopy have been powerful tools for studying molecular organization and dynamics in cells. Fluorescence Correlation Spectroscopy (FCS) has been widely applied to probing molecular dynamics in live cells with single molecule sensitivity and the ability …
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