Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"Fluorescence assay"”.
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Fluorescence assay for polymerase arrival rates
… for cytosine. Pyrrolo-dC shows a detectable fluorescence difference when in single-stranded versus double-stranded DNA. During transcription, RNA polymerase separates the two strands of DNA, leading to a change in the fluorescence of pyrrolo-dC. By incorporating pyrrolo-dC at specific …
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The Development of a Time-Resolved, Vesicle-Based, Fluorescence Assay for the Activity of the Lipid Kinase Pik1
… the Bellbrook Labs Transcreener ADP2 FI Assay kit, a fluorescence-based enzyme assay kit that reports the production of ADP, was adapted to create a time-resolved, vesicle-based assay. The assay was developed and validated with the catalytic subunit of Protein Kinase A (PKA) and the …
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THE OPTIMIZATION OF A TIME-RESOLVED, VESICLE-BASED FLUORESCENCE ASSAY FOR THE ACTIVITY OF THE LIPID KINASE PI4K-IIIBETA AND THE EFFECT OF LIPID TRANSFER PROTEINS ON ENZYME ACTIVITY
… human PI4K-IIIβ in Escherichia coli and assayed lipid kinase activity using an optimized real-time, vesicle-based fluorescence assay. After comparing different affinity tags, deletion mutants and expressing cell lines, GST-tagged wild-type PI4K-IIIβ was chosen and expressed in Rosetta …
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Aminocyclodextrin Ion Channels: Development of a Model for Ligand Gating and Investigations of the Effects of Channel Structure on *Transport Properties and Ion Selectivity
… ion transport properties were assessed with a fluorescence assay using the pH sensitive dye HPTS. The amino acid containing channel showed little activity, while the di- and tetraamino channels showed increasing activity with increasing substitution. The tetraamino channel showed selectivity …
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The photophysics properties of lanthanide luminescent probes
… have been carried out using a time- resolved fluorescence assay (TRFA). Bi-cs124 was conbined with different chelating agents, specifically diethylene triamine pentaacetic acid (DTPA), ethylene diamine tetraacetic acid (EDTA), ethylene glycol tetraacetic acid (EGTA), and 3,6,9,12-Tetrakis …
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Development of Ultra-Sensitive Fluorescence Photoamplification Assays for the Detection of Molecular Recognition Events
… of dithiane with a "turn-off" or "turn-on" fluorescence-based assay for the detection of biological targets and ligands, regardless of their nature, through a molecular recognition event has been developed. This research has included several key steps, the most significant being: (1) the …
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Investigation of CNT-induced Escherichia coli Lysis and Protein Release
… various levels of agitation for different times. Fluorescence assay for GFP, optical absorbance for -lactamase activity, and Transmission Electron Microscopy (TEM) were used to determine the amount of released protein, and visually examine the permeability enhancement of the cells, respectively. …
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Characterization of protein induced fluorescence enhancement (PIFE): A label-free protein assay with short distance sensitivity
… In this report, we report on a newly developed fluorescence assay termed Protein Induced-Fluorescence Enhancement (PIFE), which circumvents protein labeling and displays sharp detection sensitivity in short distance regions, overcoming the inherent limitations of FRET. The enhancement of …
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Cell-free platform for the functional characterization of intracellular ion transporters
… of CFPS with two functional readout methods: a fluorescence assay in liposomes and an SLB bioelectronic platform. With these tools, we can directly detect the activity of difficult-to-study membrane proteins like intracellular ion transporters. Additionally, we can further characterize membrane …
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Single molecule fluorescence spectroscopy of ClpXP-mediated substrate degradation
… occurs. Here, we present the single molecule fluorescence assay for probing the interaction between the ClpXP enzyme and its substrates. A covalently crosslinked ClpX hexamer maintain functionally stable form at the low concentration of single molecule level. Surface passivation through …
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Characterization of the Morphology, Composition, and Activity of the Germ Plasm in Nasonia vitripennis
… oosome. Using a combination of super-resolution fluorescence microscopy and transmission electron microscopy, I identified lipid droplets and ribosome-associated vesicles widely distributed throughout the oosome interior, where mitochondria are notably absent. I also identified two patterns of …
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Synthesis and Evaluation of the Aristotelia Alkaloids at the α3β4 Nicotinic Acetylcholine Receptor
… the α3β4 nAChR by developing two complementary assays to evaluate novel antagonists based on the ARQ scaffold: a calcium-based fluorescence assay and automated patch-clamp electrophysiology. Extensive subsequent analogue synthesis and assessment has identified compounds with enhanced potency and …
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Design of potential antiprotozoal daunorubicin derivatives
… with other types of linkage were inactive. A fluorescence assay method was developed to measure drug release from conjugates. Investigation revealed that glutaraldehyde linked conjugate released about 20% of bound drug over a 2-3 hour period when incubated in murine plasma. In contrast, …
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Palmitoylation and Oxidation of the Cysteine Rich Region of SNAP-25 and their Effects on Protein Interactions
… the application of a biotinylation streptavidin assay to verify palmitoylation. Palmitoylation increases the extent to which SNAP-25 interacts with lipids as observed with an environment sensitive trpytophan fluorescence assay. Palmitoylation also alters the phase transition of DPPC lipids …
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Biophysical characterization of KO513, a protein expressed in human invasive glioblastoma
… and purification of K0513. Using tryptophan fluorescence assay and limited proteolysis, nucleotides were found to have no significant effect on the tertiary structural conformation of K0513. Pull down assay shows promising interactors of K0513 from fibrosarcoma cell line; future studies will …
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Fabricating protein arrays using Dip-pen Nanolithography techniques for improved sensitivity of biomarker detection
… DPN methods were optimised to create PSA immunoassay arrays, which were detected by fluorescence microscopy and gave good limits of detection (LOD). Furthermore, the assay method proved adaptable, as consistent results were obtained from two different functional glass substrates. To improve the …
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Generierung von Transposonmutanten des Epstein-Barr-Virus zur Charakterisierung von Genfunktionen für die lytische Virusvermehrung und die virale Latenz
… BHRF-1, EBNA-1, -2, -3A-C, LMP1, -2) and immuno fluorescence assay (EA-D, LMP-1). The cells were analyzed morphologically and with regard to a modulated expression of surface markers (CD11a, CD23, CD30, CD40, CD44, CD54, CD58). Wildtype EBV infected cells and uninfected cells were used as …
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Synthesis of Novel Sulfonamide-Based Calpain Inhibitors and Their Potential as Anti-Tumor Agents
… of <strong>1 </strong>were synthesized and assayed for their ability to inhibit µ-calpain utilizing a kinetic fluorescence assay and for their anti-tumor ability by SRB colorimetric assay (Chapter 3). Introduction of the electrophilic functionality significantly enhanced calpain inhibition. …
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Enhancing the Industrial Potential of Filamentous Cyanobacteria
… in dilute culture media, a dual-stained fluorescence assay was evaluated. While the viable cell stain (SYTO® 9) was accurate, the non-viable cell stain (propidium iodide) also bound to viable cells. Additional non-viable cell stains were evaluated, but none were accurate at quantifying …
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Single molecule fluorescence studies of biomolecular interactions
… have largely been confined by limitations in assays to studying the interaction between simple DNA substrates and a single protein. During my PhD I developed several novel assays to study a long-standing controversial biophysics question (flexibility of short dsDNA), genome packaging in …
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