Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 78 for “"Fluorescence Resonance Energy Transfer (FRET)"”.
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Fluorescence Resonance Energy Transfer (FRET) Based Sensors for Bioanalysis
… characterize new methods and sensors based on fluorescence resonance energy transfer (FRET) for bioanalysis. Chapter 3 describes the use of FRET between donor fluorophores and acceptor labeled murine macrophage cells. FRET microscopy was used to determine whether the donor molecules truly …
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Fluorescence Resonance Energy Transfer (FRET) systems for biomedical sensor applications
This thesis investigates the use of Fluorescence Resonance Energy Transfer (FRET) for biomedical sensor applications. FRET is a process by which energy is transferred, via long range dipole-dipole interactions, from a donor molecule (D) in an excited electronic state to an acceptor molecule (A). …
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Study Of Protein-Rna Interactions Using Fluorescence Resonance Energy Transfer (fret) And Single-Molecule Fret
… of RNPs are poorly understood. Single-molecule Fluorescence Resonance Energy Transfer (smFRET) techniques that provide information about heterogeneity and dynamic behaviors of molecules have been developed to investigate inter- and intra-molecular interactions. Here we have used FRET in …
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An Investigation of the Phospholamban-Serca Regulatory Interaction with Fluorescence Resonance Energy Transfer
<p>With Fluorescence Resonance Energy Transfer (FRET), we are able to detect changes in the structure and affinity of the PLB-SERCA regulatory complex in live cells. Using this approach, we have detected a high level of PLB-SERCA interaction even at Ca2+ concentrations known to fully relieve PLB …
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Methodology development for imaging histone modifications and for site-specific protein labeling in vitro and on the surface of living cells
… histone phosphorylation and methylation by fluorescence resonance energy transfer (FRET). These reporters are four-part chimeric proteins with the domains from N- to C-terminus as follows: a cyan fluorescent protein (CFP), a phosphoserine recognition domain (14-3-3 tau) or a methyllysine …
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Single-cell reporters for inflammatory caspase activity
… we generated 2 inflammatory caspase reporters: a fluorescence resonance energy transfer (FRET) inflammatory caspase activity reporter as well as a fluorescent translocation inflammatory caspase reporter. These reporters were then used to study inflammatory caspase activity in vitro using …
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Development of an in-cell förster resonance energy transfer technique to study protein structure inside living cells
<p>The goal of my thesis is to develop an in-cell fluorescence technique that allows for measurement of the distances between fluorescence acceptors and donors within a protein or between two proteins inside the correct intracellular compartment of living cells. The successful achievement of this …
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Development of a fluorescence resonance energy transfer optical nanoscale biosensor based on a liquid-core waveguide platform
… optical biosensor architecture utilizing fluorescence resonance energy transfer (FRET) is proposed. This biosensor architecture is then applied to a highly adept liquid-core waveguide platform. FRET is a distance-dependent signal transduction method that occurs between two fluorescent …
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Dynamics of Hairpin Ribozyme
… our investigation of dynamic processes using fluorescence resonance energy transfer (FRET) techniques on single molecules. The functional blocks and dynamic mechanisms when unveiled would help us understand RNA catalysis.
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Part A The application of fluorescence resonance energy transfer to reaction discovery Part B The stereodefined synthesis of tetrasubstituted olefins
… for enantioselective reaction monitoring using Fluorescence Resonance Energy Transfer (FRET) is described. The syntheses of enantiomeric epoxides bearing different fluorescent donors and a phenol that bore fluorescence acceptor were explored.* Part B describes the stereodefined synthesis of …
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Advancing fast relaxation imaging to determine protein stability and folding kinetics on poly(n-isopropyl acrylamide) films
… protein unfolding in situ by imaging changes in fluorescence resonance energy transfer (FRET) after fast temperature perturbations. The protein used in experiments is phosphoglycerate kinase (ePGK1), which undergoes cooperative unfolding. Contrary to previously accepted notions, the melting …
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Fluorescence resonance energy transfer-based biosensors for monitoring prostate specific antigen
… of localized regions of prostate tissue. Using fluorescence resonance energy transfer (FRET), several peptide substrates have been designed to respond to varying concentrations of PSA with a concomitant increase in fluorescence. In the near-infrared wavelength range, these fluorescent substrates …
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In vivo visualization of CaMKII activity in ocular dominance plasticity
… Camui, which utilizes the optical phenomenon of fluorescence resonance energy transfer (FRET), to monitor CaMKII activation. This thesis embodies the work done to improve Camui to be a better tool for in vivo reporting of CaMKII activity, as well as the use of this improved probe for in vivo …
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Surface Optochemical Sensors
… 5 describes development for the first time of a fluorescence sensor to measure the proteolysis activity of pancreatic cancer cells in microfluidic systems. The sensor was fabricated using a Layer by layer (LbL) deposition of polyelectrolyte. The sensor was based on Fluorescence Resonance Energy …
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Macromolecular crowding effects on the excited-state dynamics of novel FRET probes using time-resolved fluorescence
… in live cell studies. Recently, several fluorescence-based probes have been engineered to potentially quantify crowding within living cells. These dynamic probes contain a pair of fluorophores (mCerulean3 and mCitrine, the donor and acceptor, respectively) that are capable of fluorescence …
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Structural and mechanistic studies on antimicrobial peptides that target multi-drug resistant bacteria
… and tested using a recently developed fluorescence resonance energy transfer (FRET) assay developed by the Caffrey group.
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Analyse und Visualisierung eines R2R3-MYB / bHLH Transkriptionsfaktorkomplexes aus Arabidopsis thaliana
… analyzed with new methods from the physics like fluorescence correlation spectroscopy (FCS) or fluorescence resonance energy transfer (FRET). With FCS and recombinant expressed proteins, promoter attachment sites of the transcription factor complex were identified. Simultaneous binding of both …
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A computational approach for the discovery and characterization of oligonucleotide aptamers for protein targets
… molecular dynamics simulations, and free energy calculations made it possible to develop the in-silico workflow. The work reported in this thesis covers the computational tools, algorithms, and methods developed and implemented to select high-affinity aptamers for SARS-CoV-2 nucleocapsid …
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Untersuchungen zur Produktion und Chaperoninteraktion von Yersinia YopE und SycE mittels Reporterfusionstechnologie
… mit GFP und BFP sollte durch einen Fluorescence Resonance Energy Transfer (FRET), bei dem die Fluoreszenzenergie von BFP strahlenlos auf GFP übertragen wird wenn beide Proteine in einer Distanz von 1-10 nm liegen, die Interaktion der beiden Proteine intrazellulär dargestellt werden. …
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Flourescent Biosensor-Based, Cdc42 Activity Imaging For Understanding the Regulation of Epidermal Growth Receptor (EGFR) Signalling in Head and Neck Cancer
… optical imaging techniques such as, Fluorescence Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FUM) I have analysed Cdc42 activity in Squamous Carcinoma Cell Lines after EGFR tyrosine kinase inhibitor (TKI) treatment, using the FRET biosensor …
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