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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.

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Showing 1 to 20 of 117 for “"Fluorescence Lifetime"”.

  1. Fluorescence lifetime distributions in proteins

    Alcala, Jose Ricardo2011

    … was developed to measure the natural fluorescence decay of single tryptophan residue proteins. The instrumentation uses mode-locked, synchronously pumped, cavity dumped and frequency doubled dye laser excitation. The detectors are modulated phase coherently with the mode-locked laser …

    uiuc Repository record

  2. Compressed fluorescence lifetime imaging microscope

    Ma, YayaoUniversity of Illinois at Urbana-Champaign2019

    Fluorescence lifetime imaging microscopy (FLIM) has been extensively applied in wide biomedical applications from single cell studies to medical diagnosis. While the state-of-the-art FLIM techniques are highly sensitive to biological dynamics such as fluorescence resonance emission transfer (FRET), …

    uiuc Repository record

  3. Advancing fluorescence lifetime microscopy for quantitative bioimaging

    Poudel, ChetanUniversity of Cambridge2021

    This thesis presents the development of fluorescence lifetime imaging microscopy (FLIM) methodologies to advance biological understanding of neurodegeneration and stem cell differentiation. Two FLIM technologies enabling faster FLIM acquisition were developed and tested for high-throughput …

    cambridge Repository record

  4. LINEAR AND NONLINEAR FLUORESCENCE LIFETIME IMAGING FOR BIOSENSING APPLICATIONS

    Damayanti, Nur Pradani

    Fluorescence techniques, based on both linear and non-linear excitation, have been widely used in biosensing application. Among fluorescence techniques, Fluorescence Lifetime Imaging (FLIM) has gained increased importance because of their robust and versatile feature. FLIM is a technique to …

    purdue-thes Repository record

  5. Computational Dead Time Correction in Fluorescence Lifetime Imaging Microscopy (FLIM)

    Yin, Yidan2027

    <p>Fluorescence Lifetime Imaging Microscopy (FLIM) is a crucial tool in biomedical research. It can visualize and measure molecular interactions and cellular processes by analyzing the fluorescence decay of molecules in biological samples. Unlike traditional imaging methods based on fluorescence …

    wustl Repository record

  6. Advanced Fluorescence Lifetime Imaging and Spectroscopy Techniques for Biological Samples

    Chung, Pei-HuaKing's College London2012

    … and chemical signalling. In this thesis, fluorescence-based studies were conducted on a fluorescent molecular rotor, BODIPY-Ci2, which was used to measure the intracellular viscosity. BODIPY-C12 is insensitive to the surrounding polarity using solvatochromic methods. It was demonstrated …

    kings Repository record

  7. Development & Application of Fluorescence Spectroscopy & Fluorescence Lifetime Imaging to Biology & Medicine

    Spring, Bryan QuiltyUniversity of Illinois at Urbana-Champaign2015

    … (FRET) and development and application of fluorescence lifetime-resolved imaging microscopy (FLIM). It includes work to extend the FRET and FLIM methods for studying microsecond reaction kinetics. The common thread and unifying theme of the dissertation is the development and application of …

    uiuc Repository record

  8. The development of fluorescence lifetime imaging and an application in immunology

    French, Todd EugeneUniversity of Illinois at Urbana-Champaign2011

    Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:35:59Z Item is restricted indefinitely.

    uiuc Repository record

  9. Fluorescence lifetime-resolved imaging microscopy studies: quantitative image analysis, spectral-FLIM, and photosynthesis

    Chen, Yi-ChunUniversity of Illinois at Urbana-Champaign2010

    … and image analysis methods for the fluorescence lifetime-resolved imaging microscopy (FLIM) were carried out in this thesis. With the new instrument setup and imaging processing algorithms, valuable information has been provided for studying in vivo photosynthesis activities. Chapter …

    uiuc Repository record

  10. Development of fluorescence lifetime imaging microscopy techniques for analysis of single extracellular vesicles

    Sorrells, Janet EliseUniversity of Illinois at Urbana-Champaign2020

    … This thesis presents the first use of two-photon fluorescence lifetime imaging microscopy (FLIM) to characterize the free and protein-bound reduced forms of nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate (NAD(P)H) content of EVs derived from cells, human urine, …

    uiuc Repository record

  11. Applying Flinet Deep Learning Model to Fluorescence Lifetime Imaging Microscopy for Lifetime Parameter Prediction

    Odezuligbo, Ikenna EmmanuelCreighton University2024

    Fluorescence Lifetime Imaging Microscopy (FLIM) is essential in biomedical research, providing insights into cellular mechanisms and disease diagnosis by measuring the time molecules remain in an excited state before emitting a photon. Analyzing FLIM data is challenging due to the complex, …

    creighton Repository record

  12. Development &Amp; Application of Fluorescence Spectroscopy &Amp; Fluorescence Lifetime Imaging to Biology &Amp; Medicine

    Spring, Bryan QuiltyUniversity of Illinois at Urbana-Champaign2014

    … (FRET) and development and application of fluorescence lifetime-resolved imaging microscopy (FLIM). It includes work to extend the FRET and FLIM methods for studying microsecond reaction kinetics. The common thread and unifying theme of the dissertation is the development and application of …

    uiuc Repository record

  13. Fast Fluorescence Lifetime Imaging Using a Full-Field Homodyne System with Applications in Biology

    Redford, Glen Ivan2012

    A fast, full-field fluorescence lifetime imaging system based on the homodyne technique was built and used for several applications. Also contained herein is the basic theory and background for fluorescence lifetime imaging, as well as data analysis of lifetime distributions. The fast, easy-to-use …

    uiuc Repository record

  14. Fluorescence lifetime imaging microscopy to study protein aggregation in the context of neurodegenerative diseases

    Chung, Chyi WeiUniversity of Cambridge2022

    … presented in this thesis involves the use of fluorescence lifetime imaging microscopy (FLIM) and other biophysical techniques to elucidate the molecular mechanisms that underlie different amyloid-associated neurodegenerative dis- eases. Currently, there is no prevailing therapeutic option for …

    cambridge Repository record

  15. Light in Living Systems: Ultra-Weak Photon Emission and Fluorescence Lifetime Imaging of Cellular Dynamics

    Samuel Crowther (21041456)2026

    … Part IV extends optical interrogation into the fluorescence regime, employing dual dye Förster resonance energy transfer (FRET)-fluorescence lifetime imaging microscopy (FLIM) to probe mitochondrial nano-organisation. Dual staining with MitoTracker^(TM) Green FM (MTG) (donor) and …

    exeter

  16. Simulation and Characterization of Single Photon Detectors for Fluorescence Lifetime Spectroscopy and Gamma-ray Applications

    Benetti, MicheleUniversità degli studi di Trento2012

    Gamma-ray and Fluorescence Lifetime Spectroscopies are driving the development of non-imaging silicon photon sensors and, in this context, Silicon Photo-Multipliers (SiPM)s are leading the starring role. They are 2D array of optical diodes called Single Photon Avalanche Diodes (SPAD)s, and are …

    trento Repository record

  17. Determining S6K1 localisation and interactions with mTORC1 in live cells using fluorescence lifetime imaging microscopy

    Ahmed, Abdullah R.Oxford Brookes University2018

    … used to investigate their cellular action. The fluorescence quantum yields for AZD2014 and INK128 are 0.47 and 0.33, respectively. Cellular uptake of the drugs is rapid with a half-life of 60 seconds and 42 seconds, respectively. Both drugs localise to mTORC1 related sub-cellular sites. Using …

    oxford-brookes Repository record

  18. Development of the single-molecule tracking and fluorescence lifetime techniques for biophysical measurements and biomedical applications

    Chen, Yuan-I, Ph. D.The University of Texas at Austin2022

    … 3D single-molecule tracking (3D-SMT) and lifetime measurement methods that can follow individual DNA molecules diffusing inside a mammalian cell and observe multiple annealing and melting events on the same molecules (Chapter 2). By comparing the hybridization kinetics of the same DNA …

    texas Repository record

  19. Development of a Fluorescence Lifetime Based Method to Detect and Analyze Single Molecule Reactions in Solution

    Colyer, Ryan AnthonyUniversity of Illinois at Urbana-Champaign2015

    This new hardware was used to observe the conformational dynamics of a calmodulin sample labeled with a FRET pair, and encapsulated within 100nm lipid vesicles. A toolbox of analysis techniques was developed for PTA, and was used to quantitatively describe the transitions and fluctuations of the …

    uiuc Repository record

  20. Optimization of fluorescence lifetime imaging microscopy (FLIM) for studying the activity of enzymes in live cancer cells

    Eichorst, JohnUniversity of Illinois at Urbana-Champaign2013

    … describes the process of optimizing a Fluorescence Lifetime Imaging Microscopy (FLIM) system in order to observe the dynamics of enzymes in live cancer cells. The enzyme studied throughout this research is Membrane Type 1 Matrix Metalloproteinase (MT1-MMP) which is a membrane-bound …

    uiuc Repository record

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