Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 40 for “"Fluorescence In Situ Hybridization (FISH)"”.
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Generalist 3D Cell Phenotyping for All-Type Tissues
Tissue-clearing methods, light-sheet microscopy, and antibody labeling enable extracting cellular and subcellular information, producing large amount of image data needs to be analyzed. Hundreds of heterogeneous cell types were detected through the data obtained across species and types of tissues. …
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From coarse fate choice to precise pattern: post-mitotic progenitor targeting
… these progenitors migrate across the animal remains unclear. Irradiation experiments eliminate all neoblasts outside shielded areas, allowing for the visualization of cell migration from the remaining neoblasts, but irradiated animals may not reflect homeostatic progenitor migration patterns. To …
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Simultaneous partial Nitritation, Anammox and Denitrification (SNAD) process for treating ammonium-rich wastewaters
… received a great deal of attention during the last years. Since the discovery of anammox bacteria the traditional theory of biological nitrogen removal has been extended to new nitrogen metabolic pathways. This thesis deals with the study of Simultaneous partial Nitritation, Anammox and …
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Characterization of genetically distinct neuronal subtypes in the lateral habenula using Cre mouse lines
The lateral habenula (LHb) is a bilateral brain structure implicated in action selection, reward processing, and motivation. The LHb projects to the ventral tegmental area (VTA) and dorsal raphe nucleus (DNR), and is thus involved in both dopaminergic and serotonergic pathways. Recent single-cell …
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Pressure Treatment of Polytene Chromosomes and Computer Vision Techniques for High Resolution Cytogenetic Studies
The exceptional cytology provided by the interphase chromatin organization in polytene chromosomes has made Drosophila the premier model organism for analysis of chromosome structure and behavior. A major impediment to full exploitation of polytene chromosome cytology has been the difficulty in …
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Quantitative Erfassung von Bakterien und deren spezifische Aktivität in komplexen Habitaten mittels Fluoreszenzmikroskopie und digitaler Bildverarbeitung
… was to develop a procedure for both the determination of the concentration of bacteria and their specific activity in complex habitats. Because of the deficiency of common used techniques for the determination of total counts, the total sytox count was developed. The new procedure consists of …
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THE EFFECTS OF AGE AND HETEROCHROMATIN ON FREQUENCIES OF ACQUIRED CHROMOSOMAL ANEUPLOIDY IN UNCULTURED HUMAN LEUKOCYTES
… relationship between autosomal loss and age remains unclear. The emergence of the specific and highly sensitive fluorescence in situ hybridization (FISH) technology has enabled investigators to study interphase cells, thereby overcoming problems inherent with the study of metaphase spreads for …
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Caracterización clínica de pacientes pediátricos con síndrome de deleción 22q11 atendidos en Hospital de Bogotá, durante el periodo de 2010 a 2020
… la segunda causa de cardiopatía congénita en la infancia (1). El 90-95% de los casos se deben a microdeleciones de novo, con un riesgo de recurrencia de ∼1%. El 22q11DS afecta múltiples sistemas que incluyen el sistema cardiaco e inmunológico, alteraciones faciales y dificultades en el …
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Processes and community structure in microbial biofilms of the River Elbe: relation to nutrient dynamics and particulate organic matter
The conceptual subject of this study was to investigate the effects of microbial biofilms of the hyporheic zone of the river Elbe on nutrient dynamics and elimination. This resulted in four aspects, which were carried out separately. One aspect was the investigation of the dynamics of inorganic …
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Greining erfðamengja xTriticoleymus og Rosa L.
Melhveiti (xTriticoleymus) er blendingur hveiti (Triticum) og melgresis (Leymus) tegunda. Erfðamengi xTriticoleymus er áhugavert vegna þess að blendingurinn er frjór og mögulega hægt að rækta hann sem nytjaplöntu. Í þessari rannsókn voru litningar einangraðir úr rótarendum xTriticoleymus og …
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Metakaryotic biology : novel genomic organization in human stem-like cells of fetal-juvenile development and carcinogenesis
Eight distinct nuclear shapes, or morphologies, have been discovered in human proto-organs and tumors, including bell-shaped nuclei with stem-like properties. These bell-shaped, or "metakaryotic," nuclei are abundant in fetal tissues and neoplasias, but rare in normal adult somatic tissues. …
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Nitrification process in integrated fixed-film activated sludge (IFFAS) system
… ammonia released from wastewater is a key factor in detrimental environment concerns such as eutrophication. Nitrogenous waste is most commonly removed from wastewater through biological means, typically involving the use of ammonia oxidizing bacteria (AOB), nitrite oxidizing bacteria (NOB), and …
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An automated multicolour fluorescence in situ hybridization workstation for the identification of clonally related cells
The methods presented in this study are aimed at the identification of subpopulations (clones) of genetically similar cells within tissue samples through measurement of loci-specific Fluorescence in-situ hybridization (FISH) spot signals for each nucleus and analyzing cell spatial distributions by …
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Ecology of Culturable Organisms at Rozel Point, Great Salt Lake, Utah
… organisms from extreme environments is an emerging field of research with applications to multiple scientific areas. One of these extreme environments is Great Salt Lake (GSL), whose microbiology has yet to be extensively studied. This dynamic and unique environment offers an excellent …
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Oligodendrocytes of the adult hypothalamic median eminence
The median eminence (ME) of the hypothalamus is a dynamic structure able to rapidly respond to nutrient availability. Both immature oligodendrocyte precursor cells (OPCs) and mature myelinating oligodendrocytes (MOLs) are found in this region in adults. While proliferation of ME OPCs has been shown …
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Evaluation of Macrolide-Lincosamide-Streptogramin B Antimicrobial Resistance at Swine Farms
Antimicrobial use in animal agriculture contributes to increased levels of antimicrobial resistance in the environment. To better evaluate this concern, the levels of macrolide-lincosamide-streptogramin B (MLSB) antimicrobial resistance at swine farms were quantified. A variation of fluorescence in …
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Phenotypic diversity within two toxic dinoflagellate genera : environmental and transcriptomic studies of species diversity in Alexandrium and Gambierdiscus
Dinoflagellates are a diverse group of single-celled eukaryotic phytoplankton that are important for their unique genetics and molecular biology, the multitude of ecological roles they play, and the ability of multiple species to produce toxins that affect human and ecosystems health. Two …
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Phenotypic diversity within two toxic dinoflagellate genera : environmental and transcriptomic studies of species diversity in alexandrium and gambierdiscus
Dinoflagellates are a diverse group of single-celled eukaryotic phytoplankton that are important for their unique genetics and molecular biology, the multitude of ecological roles they play, and the ability of multiple species to produce toxins that affect human and ecosystems health. Two …
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Microfluidic-based Fluorescence Detection for Biomarker Analysis
In this dissertation, 2 platforms are described with the combination of microfluidics and fluorescence detection for biomarker analysis. The first platform described here is to isolate CTCs with high purity by using 2-stage sorting technique and herringbone features for aliquot stretching, …
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