Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"Fluorescence In Situ Hybridisation"”.
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Optimisation of interphase fluorescence in situ hybridisation for detection of common aneuploidies
… of a simple, reliable and practical method of interphase FISH which allows prenatal diagnosis of major chromosome aneuploidies using a minimum volume of amniotic fluid sample was the overall objective of this study. When all the probes required were available, the study continued by developing …
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The added value of BAP1 immunohistochemistry and fluorescence in situ hybridisation for CDKN2A/p16 and NF2 in the diagnosis and prognostication of pleural mesothelioma
… palliative. The malignancy can be challenging to diagnose, commonly due to scant samples, often requiring patients to undergo multiple procedures before a definitive diagnosis can be reached. In more recent years ancillary tests based on the common molecular alterations in the disease have …
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Mikrobielle Ökologie N-Acyl-L-Homoserinlacton-produzierender Bakterien in der Rhizosphäre von Tomatenpflanzen
N-Acylhomoserinlactones (AHL) are signalling molecules in gram-negative bacteria, which regulate, in a cell density dependent way, important interactive functions. This phenomenon is known as quorum-sensing. This work characterised the microbial ecology of the autoinducer (AHL) producing bacteria …
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Halogenated aliphatic and aromatic aerobic biodegradation via direct metabolism and cometabolism: batch and continuous tests
In this thesis the application of biotechnological processes based on microbial metabolic degradation of halogenated compound has been investigated. Several studies showed that most of these pollutants can be biodegraded by single bacterial strains or mixed microbial population via aerobic direct …
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Spatial organisation of proto-oncogenes in human haematopoietic progenitor cells
… is a highly organised organelle, with distinct specialised sub- compartments responsible for specific nuclear functions. Within the context of this functional framework, the genome is organised, allowing contact between specific genomic regions and sub-compartments. Previous work has shown …
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Acquired abnormalities of chromosome 21 in acute lymphoblastic leukaemia
The intrachromosomal amplification of chromosome 21 (iAMP21) was identified<br/>as a novel and prognositically important acquired chromosomal abnormality in<br/>childhood acute lymphoblastic leukaemia (ALL). It is defined by multiple copies<br/>of the RUNX1 gene, as seen by fluorescence in situ …
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Modelling metabolic landscapes in human naive and primed embryonic stem cells
… mammalian embryos undergo remarkable changes in their metabolism, with a global transition from oxidative phosphorylation to glycolysis as they transit out of pluripotency and begin to make the first cell fate decisions in preparation for gastrulation. In humans this transition can be modelled …
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Juvenile pilocytic astrocytomas: A search for prognostic markers
Introduction: Juvenile pilocytic astrocytomas are one of the most frequent central nervous system tumours occurring in children. While they are classified as WHO Grade I tumours, their natural progression is difficult to predict with some patients suffering significant morbidity and mortality …
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Aspects of functional constipation - a trial of an opioid antagonist as a treatment and an investigation of the colonic microbiota associated with the illness
INTRODUCTION Functional constipation (FC) is a common condition which affects patients’ quality of life (QoL), is of uncertain pathophysiology, and is poorly treated. The aims of this research were to conduct a randomised, placebo-controlled trial investigating an opiate antagonist (Naloxone - …
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Role of Amplification of Chromosome 6P12-P21 in Human Osteosarcoma
… the most common malignant tumour of bone, predominantly occurring in adolescents. Currently, the mainstay of treatment is surgical resection in combination with multi-agent chemotherapy. Understanding of the genetic basis of osteosarcoma has been impeded by the complex and heterogeneous nature of …
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A Molecular Cytogenetic Profile of the Chronic Myeloproliferative Disorders
… Myeloproliferative Disorders (CMPD) form a continuum of malignancies, the molecular pathogenesis of which remains obscure. Chronic Myeloid Leukaemia (CML) is the only disorder with a recognisable pathogenomic abnormality, the Ph chromosome. The remaining disorders have as yet, no defined disease …
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Piezo1-dependent mechanical regulation of chemical signalling in the developing Xenopus laevis brain
During brain development, neurons extend axons to distant regions. These axons are guided towards their correct targets by various chemo-mechanical signals. Major chemical signals include semaphorins, slits, netrins and ephrins/Eph, whereas mechanical signals include tissue stiffness, viscosity and …
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Fusion genes in breast cancer
… are a common and important feature in haematological malignancies, but have until recently been seen as unimportant in epithelial cancers. The discovery of recurrent fusion genes in prostate and lung cancer suggests that fusion genes may play an important role in epithelial …
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Functional characterisation of cohesin subunit SMC3 and separase and their roles in the segregation of large and minichromosomes in Trypanosoma brucei
… brucei, presents an unusual karyotype in which two main classes of chromosomes, large and small minichromosomes, need to be faithfully replicated and segregated during the cell cycle. Although the large and minichromosomes are colocalised and segregated by association with the mitotic …
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Investigating lymphocyte development in the foetal thymus and modulation by the melanocortin 5 receptor
… the generation of effective protective immunity. Innate lymphoid cells and T cells play central roles in orchestrating bespoke immune reactions by producing specialised repertoires of immuno-regulatory cytokines. ILC2 and natural killer (NK) cells are generated in the bone marrow and thymus, …
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Physiological and Molecular Consequences of Large Y Chromosome Long Arm Deletions in Mice
The mammalian Y chromosome contains genes important for male sexual maturity and reproduction. The mouse Y chromosome long arm harbours a number of multi-copy genes whose absence or reduced representation has been linked to sperm defects and offspring sex ratio distortion in favour of females. …
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A Systematic Evaluation of Methods to Separate X- and Y- Bearing Sperm
This project was initiated to determine if was possible to enrich either X- or Y- bearing sperm, and hence to preselect the sex of a child. Two of the possible reasons why couple might want to select the sex of a child are firstly because of a family history of an X-linked recessive genetic …
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Genomics, Diagnosis, Novel Targeted Therapies and Characterisation of the Drivers of Relapse in Philadelphia Chromosome-Like Acute Lymphoblastic Leukaemia
… of B-cell ALL recently recognised as a distinct entity. The genomic drivers of Ph-like ALL are diverse. This thesis emphasises investigation of the JAK/STAT class of lesions. Ph-like ALL patients continue to have poor prognosis, in contrast to patients with other subtypes of B-ALL such as …
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The relationship between BRAF genetic alterations and the risk of tumour recurrence in Pilocytic Astrocytomas in children diagnosed at Red Cross Children's Hospital over a 33-year period
Introduction: Pilocytic astrocytomas (PAs), glial-derived neoplasms, are the most common central nervous system (CNS) tumours reported in the paediatric population. 1 PAs are circumscribed astrocytic gliomas associated with a favourable overall survival and thus regarded as low grade by the World …
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Genomic Diversity of Magnetotactic Bacteria from Underexplored Extreme Aquatic Habitats
… characterised by their ability to biomineralise intracellular magnetic nanocrystals within organelles called magnetosomes. These organelles assist MTB to align with geomagnetic field lines using magnetotaxis, thereby facilitating their navigation within chemically stratified aquatic …
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