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Showing 1 to 3 of 3 for “"Fluorescence Cross-correlation Spectroscopy"”.

  1. Single Molecule Ligand Binding Studies on CCR5 by Fluorescence Cross-Correlation Spectroscopy

    … to CCR5. To address this issue, we have employed fluorescence cross-correlation spectroscopy (FCCS) to determine equilibrium dissociation and inhibition constants for the RANTES analogues binding to CCR5. We developed a tandem affinity purification protocol to purify full-length, monomeric CCR5 …

    rockefeller Repository record for Single Molecule Ligand Binding Studies on CCR5 by Fluorescence Cross-Correlation Spectroscopy (opens in a new tab)

  2. Cross-talk-free dual-color fluorescence cross-correlation spectroscopy for high-throughtput screening

    … (thrombin) at the SMD level. Nucleic acid-based fluorescence sensors were used as recognition elements for the detection of single protein molecules with single-pair fluorescence resonance energy transfer. The technique provided higher analytical sensitivity compared to bulk analog measurements …

    lsu-thes Repository record for Cross-talk-free dual-color fluorescence cross-correlation spectroscopy for high-throughtput screening (opens in a new tab)

  3. Convex lens induced confinement : a novel study of weak and slow DNA & protein interactions

    … CLIC's measurements. Finally, the application of fluorescence cross correlation spectroscopy (FCCS) to the conformation fluctuations of DNA hairpins via Fdrester resonance energy transfer (FRET) is demonstrated. Progress is made towards the future use of FCCS and CLIC to determine the hairpin …

    mit Repository record for Convex lens induced confinement : a novel study of weak and slow DNA & protein interactions (opens in a new tab)