Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 64 for “"Fluorescence Activated Cell Sorting"”.
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A two-color bacterial two-hybrid system for selecting sequence-specific DNA-binding proteins via fluorescence activated cell sorting
Proteins that recognize specific DNA sequences play a major role in many biological processes, and the ability to select or design novel DNA binding proteins could have a major impact on many areas of biotechnology and medicine. This thesis begins with background information on zinc finger …
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Isolation, engineering, and characterization of intracellular antibodies specific for the huntingtin protein
… conformation which is prone to aggregation. Intracellular antibodies (intrabodies) against htt have been shown to reduce htt aggregation by binding to the htt fragment and inactivating it or preventing its misfolding. Intrabodies may therefore be a useful gene therapy approach to treatment of the …
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DESIGNING A PLATFORM FOR DEVELOPING NOVEL GLYCAN-BINDING PROTEINS USING YEAST SURFACE DISPLAY
… such as cancer, use by pathogens to infect host cells, and roles in the immune system makes them important targets for the design of therapeutics and diagnostics. However, a major limitation in targeting the vast array of glycans has been the lack of reliable reagents. Many existing …
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Characterization of Dendritic Cells in the Bovine Mammary Gland
… pathogens could potentially target dendritic cells (DC). While there has been some research describing bovine DC populations in-vitro, little is known about DC in mammary tissue. In this study, immunohistofluorescence was used to identify and localize bovine mammary DC. DC were found in …
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Cellular plasticity in white adipose tissue: in vivo identification of bipotent adipocyte progenitors in adult white adipose tissue
… stimuli can dramatically alter the cellular composition and phenotype of white adipose tissue (WAT). Nonetheless, the identity of progenitors that contribute to this cellular plasticity in vivo remains poorly understood. Utilizing genetic lineage tracing techniques in combination …
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ExperiBase : an integrated software architecture to support modern experimental biology
… that can handle gel electrophoresis, microarray, fluorescence activated cell sorting, mass spectrometry, and microscopy within a single coherent set of information object definitions. A key issue for interoperability is that common attributes are made truly identical between the different methods. …
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Cell Fate Specification and the Regulation of RNA-dependent DNA Methylation in the Arabidopsis Root Meristem
… a complex tissue capable of generating all the cell types that ultimately make up the root. The work presented in this thesis takes advantage of the versatility of high-throughput sequencing to address two independent questions about the root meristem. Although a lot of information is known …
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Forward and inverse metabolic engineering strategies for improving polyhydroxybyrate production
… engineering (FME) is a rational approach to cellular engineering, relying on an understanding of the entire metabolic network to direct perturbations for phenotype improvement. Conversely, inverse metabolic engineering (IME) uses a global, combinatorial approach to identify genetic loci that …
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Genomewide Crispr/Cas9 Screen Identifies Network of Protein Complexes That Regulate Trim24
… a genomewide CRISPR/Cas9 screen library using fluorescence activated cell sorting (FACS) to identify regulators of TRIM24. The screen was enabled by two innovations. I engineered cells with an in-frame knock-in of mClover3 to the endogenous copy of TRIM24 to allow fluorescent monitoring of …
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„Ex vivo” Replikation des pathogenen Prion Proteins
… konnte durch den Western Blot (Wb) und eine fluorescence activated cell sorting (FACS) Analyse gezeigt werden, sowie die Anreicherung des PrP durch die Circulardichroismus (CD) - Spektroskopie. Das so reguliert exprimierte PrPC wurde mit PrPSc infiziert. Die anschließende …
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Alendronate Alters Single Cell Gene Expression of Cortical Osteoblast Lineage Cells During Bone Loss
… it difficult to examine specific populations of cells which are integral to the tissue using traditional molecular methods. For this study we examined single cell cortical osteoblasts derived from the femurs of C57BL/6J mice. After isolating single cells from bone, we were able to individually …
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Advanced image processing methods for 3D high-throughput microscopy
… algorithm for large-scale 3D imaging of cells and tissues. It automates: (1) selecting in-focus images, (2) detecting features from labelled cells to diffraction-limited puncta, and (3) analysing spatial correlations between cells and proteins. Current tools lack the speed, precision and …
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Elucidating Astrocytic Diversity in Human Cerebral Organoids
… slice cultures derived from human embryonic stem cells (hESCs) were generated. The potential for their long-term growth permits the generation of large numbers of glial cells and allowed for a longitudinal assay of astrocytic diversity. This thesis reports the study of newfound astrocytic …
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The developmental transcriptional landscape and organizing function of the sieve element in the root meristem
… in sieve elements, specialized tube-like cells in the phloem. While several regulators of sieve element differentiation have been described, many remain unknown. Furthermore, the temporal cascade of differentiation regulation remains unclear due to limited resolution in published data. In …
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Targeting Aldehyde Dehydrogenases in NF2-null Meningioma and Schwannoma
… have been identified as markers of cancer stem cell activity and have been correlated with poor prognosis and chemoresistance in several cancers. This project aims to clarify the role of<br/>aldehyde dehydrogenase (ALDH) enzymes in meningioma tumours and evaluate their potential as future …
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Creation and establishment of transgenic mouse models for for Mecp2 gene, causing Rett syndrome
… exprimierenden Gehirnzellen durch FACS (Fluorescence Activated Cell Sorting) isoliert und weiter über Immunfärbungen konnte mit verschiedenen Markern charakterisiert. Ein Ansatz über Transcriptomics mit Mecp2+/EGFP+ Zellpopulationen beider transgener Linien könnte die Regulation von …
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Examining the roles of resident non-myogenic mesenchymal cell populations in skeletal muscle development and injury
… from both myogenic and their supportive niche cells. The muscular niche is complex. At the cellular level it is composed of a broad number of cell types including: endothelial vessels, nerve and nerve-supporting cells, resident immune populations, and a heterogenous group of non-myogenic …
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Inexpensive technologies enabling widespread utilization of image-predicated cell sorting
The most mature, widespread sorting technology, fluorescence-activated cell sorting (FACS), offers high throughput and sorts predicated on a wide range of phenotypes that can be conveyed through average cellular fluorescence or light scattering levels. However, FACS cannot detect a wide range of …
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Rational design and directed evolution of probe ligases for site-specific protein labeling and live-cell imaging
… However, in order to use these probes for live-cell protein imaging, highly specific labeling methods are required. Here, we will describe three efforts to re-engineer the E. coli enzyme, lipoic acid ligase (LplA), to catalyze the ligation of small-molecule probes onto recombinant proteins. We …
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The Omental Fat Band as an Immunomodulatory Microenvironment for Ovarian Cancer
… concerned with the mechanisms by which malignant cells circumvent cell death signaling and maintain unchecked proliferation, focus has shifted to the complex interactions between the tumor cell and the surrounding microenvironment. Ovarian cancer has one of the highest incidence-to-death ratios of …
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