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Showing 1 to 20 of 196 for “"Fluorescein"”.
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Antibody active site contributions to fluorescein ligand binding
… antibodies (Mabs) isolated in response to fluorescein (Fl) was generated and segregated based on Fl affinity. Intermediate affinity prototype Mab 9-40 (IgG1, $\kappa$; K$\sb{\rm a}$ = 3.3 $\times$ 10$\sp7$M$\sp{-1}$) possessed $>$90% active site quaternary structural homology to the …
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Fluorescence Studies of the Rabbit Anti-Fluorescein Antibody Active Site
Made available in DSpace on 2014-12-14T07:14:46Z (GMT). No. of bitstreams: 1 7821066.pdf: 6564881 bytes, checksum: aea9b2352ac0cb8a99488747ae6db8f0 (MD5) Previous issue date: 1978
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Investigation of zinc metalloneurochemistry with fluorescent sensors based on fluorescein platforms
… 2. Fluorescent Sensors for Zn2' Based on a Fluorescein Platform: Synthesis, Properties and Intracellular Distribution Two new fluorescent sensors for Zn2' that utilize fluorescein as a reporting group, Zinpyr-1 (ZP1) and Zinpyr-2 (ZP2), have been synthesized and characterized. ZP1 is …
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SYNTHESIS AND DEVELOPMENT OF SILICON ANALOG OF FLUORESCEIN FLUOROPHORES AND ESTERIFICATION CATALYSTS
… characterization of a novel silicon analog of fluorescein (silyl fluorescein) was studied in detail. The photo-physical properties and toxicity studies of these fluorophores are also discussed. Compared to fluorescein, silyl fluorescein displayed a 90 nm longer wavelength in its absorption and …
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Optical Coherence Tomography Angiography and wide-field fluorescein angiography in Diabetic Retinopathy
Obiettivo: studiare i cambiamenti nella perfusione maculare nei pazienti affetti da edema maculare diabetico e valutare la fattibilità della fluorangiografia con ad ampio campo nella pratica clinica. Materiali e metodi: in questo studio di coorte retrospettivo sono stati inclusi pazienti caucasici …
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The anti-fluorescein antibody active site: A single-chain site-specific mutagenesis study
Restriction data tranferred 2014-07-01T11:21:50-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission
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Cryoprecipitation Properties and Structural Analysis of a High Affinity Anti-Fluorescein Immunoglobulin-M Antibody
… 10\sp $ M$\sp{-1}$) murine monoclonal anti-fluorescein IgM antibody (18-2-3) exhibiting low temperature insolubility in the absence of bound ligand has served as a model to study cryoprecipitation. Insolubility of 18-2-3 at low temperature (4$\sp\circ$C) had been shown to be reversible at …
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Characterization, Expression and Genetic Analysis of Recurrent Idiotypes in the Unrestricted Anti-Fluorescein Immune Response
Eighteen monoclonal anti-fluorescein (Fl) antibodies from BALB/cV mice were characterized and 13 possessed a low affinity for Fl (K(,a) = 5 x 10('4) M('-1)) while 5 had a higher affinity (K(,a) (TBOND) 5 x 10('8) M('-1)). Eleven of the 13 low affinity clones were idiotypically cross-reactive …
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Electrophilic Affinity Labels and Fluorescent Conjugates for the Estrogen Receptor (Norhexestrol, Aziridine, Hexestrol, Fluorescein, Alkylator)
We prepared several aziridine derivatives of the potent, non-steroidal estrogen hexestrol ((3R*,4S*)-3,4-bis(4-hydroxyphenyl)hexane)) in search of an estrogen agonist that achieves selective, efficient labeling of the estrogen receptor. The electrophilic agent, aziridine, was positioned at a side …
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The distribution of different fluorescence dyes in the egg and embryo of the zebrafish (Danio rerio)
… of the effects of side chains, rhodamine b and fluorescein were chosen as basic fluorochromes, which were then modified by diverse substituents that differed in their partition coefficient, molecular size and charge. The substances tested were rhodamine b, sulforhodamine b, fluorescein, …
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The High Affinity Anti-Fluorescyl Immunoglobulin-M Response in Rabbits
… of high affinity IgM populations in the fluorescein hapten system. Fluorescein has characteristically elicited IgG antibodies of relatively higher affinity than values observed with other hapten systems. Characterization of protein-ligand interactions, solvent effects and the …
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Design, synthesis and characterization of fluorescent hyperbranched polyglycerols and organic nanoparticles with covalently bound anti-fading agents
… more stable fluorophore by covalently attaching fluorescein and anti-fading agent (AFA) moieties to hyperbranched polyglycerols (HPG). The use of HPGs as a scaffold to link fluorescein and anti-fading agents proved intractable, so a norbornyl based ROMP polymer was used instead. Through the …
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In Vivo Fluorometric Assessment of the Skin Microcirculation
… uptake model was developed to describe the fluorescein dermofluorometer signal in terms of the local blood flow and capillary permeability. The fluorescein signal is characterized by a wash-in time constant dependent on both the blood flow and permeability. Under flow-limited conditions the …
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Terpyridine Functionalized Dye-Doped Silica Nanoparticles (DDSN) for Detection of Metal Ions
… a novel 2, 2’: 2’, 6- terpyridine functionalized fluorescein doped silica nanoparticle system capable of the sensitive detection of Cu<sup>2+</sup> and Co<sup>2+</sup>. To further probe the properties of our nanoparticle system, we substitute the fluorescein dye of rhodamine and have observed …
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Design and synthesis of probes for detection of protein-protein interaction and RNA localization
… a random sequence pool. Solid-phase immobilized fluorescein and fluorescein phosphates were synthesized for the indirect selection of a fluorescein phosphatase ribozyme. A corresponding thiophosphate analog was created for the in-gel selection of a thiophosphatase ribozyme via APM-PAGE. Finally, …
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DEVELOPMENT OF A REFERENCE METHOD FOR THE ABSOLUTE MEASURING OF FLUORESCENCE IN BIOLOGICAL ANALYSIS
… reference fluorescence material used was Fluorescein. Standard reference materials of Fluorescein were used to ensure the traceability of measurements results. The work goals were to obtain: a method to prepare fluorescein solutions of calibration; characterization of fluorescence …
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The role of the thymus in the enhancement of humoral immunity by estrogen
… as its mediator. An immunizing dose of 0.25 mg fluorescein conjugated to keyhole limpet hemocyanin was found to distinguish between the anti-fluorescein responses of ovariectomized adult Lewis rats with and without estrogen replacement. This dose of immunogen was then administered to adult …
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AN INVESTIGATION INTO THE ANALYTICAL UTILITY OF CHANGES IN POLARIZATION ACCOMPANYING ANALYTICAL DERIVATIZATION REACTIONS (FLUORESCENCE)
… between primary amines and fluorescein isothiocyanate were initially studied. Changes in polarization were observed upon conjugation for many of the amines primarily due to changes in fluorescence lifetime. This reaction was also studied in varying amounts of glycerol to …
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