Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Flp/frt"”.
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Evaluation of the Recombination Efficiencies of FLP Proteins
… Cre/lox system from the bacteriophage P1 and the FLP/FRT system from the yeast Saccharomyces cerevisiae. The Cre/lox system is well-characterized and is the first choice in application of site-specific recombination system. However, some applications such as marker-free site-specific gene …
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Identification of a Novel Regulator of ER Stress-Induced Apoptosis
… was developed to screen 452 p-element lethal FRT lines generated by the UCLA Undergraduate Consortium in Functional Genomics. Drosophila is amenable for a modifier screen using the FLP/FRT technique, in which mitotic recombination is used to generate loss-of-function clones in specific …
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Towards a Molecular Genetic Analysis of Spermatogenesis in Drosophila
… second meiotic division. Finally, we designed a FLP/FRT-based screen that should allow us to directly identify loci regulating germ cell proliferation, without relying on a male-sterile phenotype. This opens up the possibility of saturating the genome for mutations affecting this key early step …
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Role of Drosophila Rap/Fzr (Cdh1) in retinal axon targeting and its interactions with Loco, Liprin-a and Ras
… levels. Mosaic analyses experiments using FLP-FRT and GAL4-UAS techniques show that Rap/Fzr functions in a cell autonomous manner. To test for possible role of other signaling molecules and interactions with Rap/Fzr, we have examined rap phenotypes in double mutant combinations with Loco, …
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Role of SH3 and Cysteine-Rich Domain 3 (STAC3) in Skeletal Muscle Development, Postnatal Growth and Contraction
… gene expression in postnatal mice through the Flp-FRT and tamoxifen-inducible Cre-loxP systems. Postnatal Stac3 deletion inhibited body and limb muscle mass gains. Histological staining and gene expression analyses revealed that postnatal Stac3 deletion decreased the size of myofibers and …
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Klonierung, molekulare Charakterisierung und konditionale Inaktivierung eines murinen Todesrezeptors für TRAIL (mTRAIL-R)
… wurden mit Hilfe des Cre/loxP-Systems und des Flp/FRT-Systems konditionale p54_mTRAIL-R defiziente Mäuse hergestellt. Die Werkzeuge, die in dieser Arbeit generiert wurden, wie lösliches p54_mTRAIL-R:Fc Fusionsprotein und konditionale p54_mTRAIL-R defiziente Mäuse, können nun in vivo für die …
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Nucleolar Stress Due to Depletion of Nopp140 in Drosophila melanogaster
… we deleted the Nopp140 gene in Drosophila using FLP-FRT recombination. Genomic PCR, RT-PCR, and immunofluorescence microscopy confirmed the loss of Nopp140, its mRNA, and protein products. Nopp140-/- larvae arrested in the second instar stage and died within 8 days. While nucleoli appeared intact …
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Investigating R gene evolution by meiotic recombination using synthetic gene clusters in Arabidopsis
… of site-specific recombination systems (CRE/Lox, FLP/FRT) to generate two alternative versions of the synthRPP1 gene clusters in vivo. These lines, as well as others being developed, will be used in future genetic crosses to identify and characterize plants expressing chimeric RPP1 genes. My …
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Funktionelle Charakterisierung von Daughter of Sevenless
… ein neues in vivo Testsystem basierend auf der Flp/FRT Flipase Rekombinase Technik etabliert. Dieses klonale Testsystem erlaubt die Expression mutierter Transgene unter der Kontrolle der dos Enhancer/Promotor Sequenzen in Klonen von Zellen, denen die endogene Dos Funktion fehlt. Die klonale …
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The role of Decapentaplegic (Dpp) in Drosophila wing development
… in mutant clones lacking Dpp signaling using FLP-FRT system. Clones double mutant for tkv and bsk did not undergo apoptosis, but recovered at very low frequencies compared to sibling clones. Here, I showed that the low recovery of tkv bsk double mutant clones are due to the extrusion of mutant …
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FLP-mediated conditional loss of an essential gene to facilitate complementation assays
… is placed between target sequences recognized by FLP recombinase from the yeast 2 μm plasmid. This construct is transformed into a plant heterozygous for a null mutation at the endogenous locus, and progeny plants carrying the excisable complementing gene and segregating homozygous knockout at the …