Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Flow chamber"”.
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Biomedical research application of a novel double-layer parallel-plate flow chamber
… Complicated designs of the conventional flow devices, however, have impeded such implementation. In the present study, we designed and developed a novel parallel-plate flow chamber (PPFC). It consists of multiple layers of different materials to adjust the required geometries of the …
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Attachment mechanisms of a novel, targeted, lipid-based, ultrasound contrast agent
… optimal storage of the agent determined. A novel flow chamber has been developed for assessment of microbubble detachment under very high WSS. The flow chamber has been calibrated to 50Pa wall shear stress (WSS) using laser Doppler anemometry (LDA). Higher WSS was achieved through the use of …
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Stimulation of angiogenesis through collagen gel by applying shear stress and interstitial flow
… of this mechanical stimulus, a parallel plate flow chamber was designed and tested to elicit a constant laminar flow onto a monolayer of endothelial cells. The goal of this research was to develop a more physiologically similar in vitro system to study the effects of shear stress on endothelial …
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A Compact Ultrasonic Airflow Sensor for Clinical Monitoring of Pediatric Tracheostomy Patients
… A compact ultrasonic time-of-flight (TOF) airflow sensor capable of attaching directly to the tracheostomy tube has been developed to address this need. The ultrasonic flow sensing principle, also known as transit time ultrasound, is a robust method that correlates the timing of acoustic …
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Zooplankton in Flowing Waters
… features. The current study assessed the role of flow in characterizing the microcrustacean zooplankton community of the Missouri River. I also designed an artificial flow chamber for observing species-specific responses to flow. Missouri River zooplankton samples and physico-chemical variables …
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Worm-shaped polymersomes and their effects on targeting efficiency
… to a target cell layer was also evaluated using flow chamber simulating blood flow.
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Stochastic dynamics of cell adhesion in hydrodynamic flow
… white blood cells experimentally investigated in flow chambers. There, specific adhesion is mediated by weak bonds between complementary molecular building blocks which are either located on the cell surface (receptors) or attached to the bottom plate of the flow chamber (ligands). The model …
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Experimental and computational studies of receptor-mediated cell detachment in shear flow
… cells to ligand-coated surfaces in viscous shear flow is a central phenomenon in many physiological and biotechnological processes. Mechanical strength of specific adhesion is generally presumed to be related to chemical affinity of receptor/ligand bonds, but no experimental study has been …
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Examination of a system for the mechanistic study of tumor cell-platelet interactions under well-defined flow conditions
… A875 melanoma cell monolayer in a parallel plate flow chamber at shear rates characteristic of the microvasculature. Epi-fluorescence video microscopy utilizing a cooled CCD camera and digital image processing were used to quantify the dynamic adhesion and aggregation of platelets. Varying the …
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Effect of shear stress on surface membrane potassium ion permeability of calf pulmonary artery endothelial cells (Potassium ion)
… dynes/cm$\sp2$, in a parallel-plate geometry flow chamber, and the radioactivity of the effluent was monitored with time. Increase in laminar shear stress, from 1 dyne/cm$\sp2$ to a higher level, resulted in a rapid transient increase in the rate constant for $\rm \sp{86}Rb\sp+$ release. The …
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Probing cellular protein complexes using single-molecule pull-down
… the protein of interest is immobilized on a flow chamber. The flow chambers are passivated using a polymer coating such that the cellular components do not bind, and the immobilized antibody specifically captures the target protein. Cell lysates are diluted to obtain isolated molecules …
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Feasibility of Echocardiographic Particle Image Velocimetry for evaluation of cardiac left ventricular filling function
… an apparent difficulty to diagnose patients. Flow visualization techniques have been commonly employed to study the development of these diseases as they relate to the filling process of the ventricles. One method, Echo Particle Image Velocimetry (Echo-PIV) is a relatively new method for …
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The Response of Preosteoblasts to Combined Shear and Thermal Stress for Bone Tissue Engineering
… stress at 1 dyne/cm2 using a parallel plate flow chamber, thermal stress in a 42°C incubator, or combined shear and thermal stress (1 dyne/cm2 at 42°C). Stress treatments were applied on Day 2, Day 6, and Day 10. To assess the early response of cells to stress treatments, we measured …
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Novel Bio-inspired Aquatic Flow Sensors
… develop and characterize two new sets of novel flow sensors. One set of sensors developed and studied in this work are flow sensors fabricated using carbon nanomaterials. These sensors are made by embedding carbon nanotubes (CNT) and carbon nanohorns (CNH) into a polymeric substrate and then …
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Antithrombotic agents under flow conditions
… proteins and blood cells under a wide range of flow rates. To mimic these conditions in vitro, a parallel plate flow chamber with human umbilical vein endothelial cells (HUVEC) or extracellular matrix (ECM) has been developed. A method to investigate thrombin generation (TG) in platelet rich …
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Optical deformability : micromechanics from cell research to biomedicine
… both beams. By incorporation into a microfluidic flow chamber, the optical stretcher has the potential to measure up to several cells per minute, taking the speed of cell elasticity measurements to a new level and predisposing it for applications in biomedical diagnostic applications. As examples, …
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NOGGIN Transport in a Model Human Epiblast
… spaces. Moreover, utilizing a microfluidic flow chamber, I unambiguously demonstrated the long-distance diffusion of NOGGIN through the extracellular medium at the apical side as well as the short-distance diffusion of BMP4 over few direct neighbors. Surprisingly, I further demonstrate that …
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Mechanotransduction of subcellular AMPK and its role in breast cancer cell migration
… cancer cells in response to interstitial fluid flow. Additionally, we explored the involvement of AMPK in breast cancer cell migration. An in-vitro three-dimensional (3D) cell culture model utilizing collagen-Matrigel matrices was used. Interstitial fluid flow was applied to the 3D cell-matrix …
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Discovery and Characterization of Small Molecule Inhibitors of Biofilm Formation in Pseudomonas Aeruginosa
… to cause biofilm detachment in both static and flow chamber experiments. P. aeruginosa strains taken from the sputum of 17 CF patients showed a similar response to elevated iron levels. Because free iron salts have toxic effects that likely preclude their wide use as clinical anti-biofilm …
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Estimating the concentration of ultrasound contrast agents using ultrasonic backscatter in vitro
… in degassed water using a beaker setup and a flow system setup. Experimentally obtained estimates of the BSC were fit to ultrasound scattering models to extract concentration estimates of either the UCAs or the glass beads. The fitting was accomplished with a Levenberg-Marquardt regression …
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