Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 886 for “"Flow Cytometry"”.
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Characterization of Photoacoustic Flow Cytometry Signals
<p>Photoacoustic flow cytometry has been utilized to clinically determine the presence of melanoma circulating tumor cells (CTCs). Further investigation was conducted into the morphology of detection signals and how they could be manipulated to allow for further classification. Novel features were …
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Light field microscopy for 3D imaging flow cytometry
… throughput. This has motivated the evolution of flow cytometry, which uses microfluidics to measure the fluorescence and scattering signals of thousands of cells per second, towards imaging flow cytometry, which allows spatial information to be collected at extremely high throughput. However, …
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Microfabricated fused silica flow chambers for flow cytometry
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1997.
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Modular Neural Networks for Analysis of Flow Cytometry Data
… developed an automated optical tool, called a Flow Cytometer (FC), to analyse samples quickly and in large quantities. The flow cytometer has<br/>successfully been applied to real phytoplankton studies. However, analysis of the data extracted from samples is still required. Artificial Neural …
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Quantitative Characterization of Conserved Noncoding Regions by Flow Cytometry
… and implementation of a novel application of flow cytometry in the quantitative characterization of CNRs. The technique couples fluorescent microscopy localization with flow cytometry distribution and expression level analysis, creating a technique more powerful than either on its own. To …
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Automated Analysis of Flow Cytometry Data for B-Cell Lymphoma
Flow cytometry, a powerful tool for the diagnosis of hematolymphoid malignancies including B-cell lymphomas, is an innovative technique that measures the fluorescence of suspended cells. Traditionally, the method of evaluating the research and clinical study of flow cytometry data is done by the …
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Identification of Human Adrenal Carcinoma Cells Using Photoacoustic Flow Cytometry
… needed in samples using photoacoustic flow cytometry (PAFC). PAFC generates ultrasonic waves when particles under flow absorb laser light. Due to the yellow color in adrenal cancer cells, we demonstrate that cells under flow optimally absorb a laser in blue/indigo color. We also …
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Physiological studies on bacterial fermentations using multi-parameter flow cytometry
… of both industrial and clinical interest. Flow cytometry was used throughout this work in order to assess the quality of recombinant Escherichia coli populations present within an agitated bioreactor. It was demonstrated in shake-flask culture that the cells could be grown to moderate cell …
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Rapid detemination of antimicrobial susceptibility using gel microdroplets and flow cytometry
Thesis (Ph. D.)--Massachusetts Institute of Technology, Whitaker College of Health Sciences and Technology, 1991.
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Flow cytometry in the diagnosis and prognosis of canine lymphoma and leukemia
… of prognosis for canine lymphoma by using flow cytometry of lymph node aspirates. Data from one hundred and sixty dogs with confirmed multicentric B-cell lymphoma were used to test the hypothesis that poor patient outcome could be predicted by tumor cells expressing a low level of class II …
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Compositional heterogeneity within oceanic POM : a study using flow cytometry and mass spectrometry
… temperature-resolved mass spectrometry (DT-MS), flow cytometry, and multivariate statistics to the study of marine particulate organic matter (POM) collected from the North Atlantic. DT-MS is an important asset to marine organic geochemistry as a single two minute analysis (with 16 eV, EI+ …
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A comparison of flow cytometry and conventional microbiology in the study of biofilms.
A comprehensive study on the application of flow cytometry (FCM) for the analysis of biofilms has been undertaken and the results presented in this thesis have shown that flow cytometry can been successfully used to enumerate, sort and image the bacteria and amoebae in biofilms and water …
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Affinity Maturation of the D1.3 Antibody Using Yeast Surface Display and Flow Cytometry
Different methodologies were explored for improving YSD affinity maturation of anti-protein antibodies, using scFv D1.3. Using equilibrium sorted YSD with the D1.3/M3 antibodies, a single-pass enrichment factor of 125-fold (+/-65-fold) was achieved, indicating excellent differentiation between …
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Mammalian cell clonal growth and secretion measurements using gel microdroplets and flow cytometry
Thesis (Ph. D.)--Harvard University--Massachusetts Institute of Technology Division of Health Sciences and Technology, Program in Medical Engineering and Medical Physics, 1989.
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A flow cytometry-based method for gene expression profiling of CNS cell types
… activated nuclei sorting (FANS). FANS utilizes flow cytometry to isolate cell nuclei from CNS subtypes and microarray analysis of nuclear mRNA. When compared to an existing technique, translating ribosome affinity purification, it was found that FANS was able to detect differentially expressed …
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Compositional heterogeneity within oceanic POM : a study using flow cytometry and mass spectrometry
Thesis (Ph. D.)--Joint Program in Oceanography, Massachusetts Institute of Technology/Woods Hole Oceanographic Institution, 1998.
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3D material cytometry (3DMaC): High-throughput, high replicate screening of materials using flow cytometry
… such methods are lacking. This thesis shows how flow cytometry can be adapted to the study of biomaterials. Flow cytometry allows for the automated collection of a large number of unique events in a short time period and is already widely used for cell analyses. Here, biomaterial, specifically …
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Development of a Flow Cytometry Method for the Identification and Enumeration of Enterococcus faecalis
<p>A multi-color flow cytometry method coupled with fluorescent staining was developed to detect and quantify <em>E. faecalis</em> concentrations in coastal waters as an alternative to the traditional membrane filtration method. The developed method was used to characterize <em>E. faecalis …
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