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Showing 1 to 20 of 33 for “"Flavoprotein"”.
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Biochemical characterization of a novel iron-sulfur flavoprotein from Methanosarcina thermophila strain TM-1
The iron-sulfur flavoprotein (Isf) from the acetate utilizing methanoarchaeon Methanosarcina thermophila was heterologously produced in Escherichia coli, purified to homogeneity, and characterized to determine the properties of the iron-sulfur cluster and FMN. Chemical and spectroscopic analyses …
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Studies on the Mechanism of the Pyruvate Oxidase Flavoprotein of Escherichia Coli (Rapid Kinetics, Biochemical Software, Lipid-Activated Enzyme)
The pyruvate oxidase flavoprotein catalyzes the oxidative decarboxylation of pyruvate to acetate and carbon dioxide. Flavin adenine dinucleotide and thiamin pyrophosphate are cofactors. The enzymatic activity of the purfied flavoprotein is very low in the absence of lipids. Following reduction by …
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Characterization of the genes and gene products of the acetate-activating enzymes and a novel iron-sulfur flavoprotein from Methanosarcina thermophila strain TM-1
The genes encoding the acetate kinase and phosphotransacetylase enzymes from <i>Methanosarcina thermophila</i> were isolated from a genomic library on a fifteen kilobase fragment The genes are located adjacent to one another, with the phosphotransacetylase gene (<i>pta</i>) directly upstream of the …
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Genetic and Biochemical Characterization of the Heteromeric Dihydroorotate Dehydrogenase From Bacillus Subtilis
… a heteromeric DHOD holoenzyme, an iron-sulfur flavoprotein which was determined by gel filtration to be a tetramer containing 2 mol PyrDI and 2 mol PyrDII. The two subunits were also overexpressed individually and purified. Overexpressed PyrDII formed inclusion bodies and could be purified by …
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Developing assays to study and screen engineered Escherichia coli NADPH-dependent sulfite reductase
… higher order structure consists of an oxidative flavoprotein subunit and a reductase hemoprotein subunit that assemble in a unique asymmetrical stoichiometry to efficiently coordinate the large-volume electron transfer reaction. This oxidoreductase lends itself to numerous design opportunities …
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Stretch induced hyperexcitability of mice callosal pathway
… neural responses to mechanical stretching. Flavoprotein autofluoresence (FA) imaging was employed for measuring neural activity. We observed that synaptic excitability substantially increases after a small (2.5\%) stretch was held for 10 minutes and released. The increase is accumulative, …
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A kinetic and equilibrium description of camphor hydroxylation by the P450cam monoxygenase system
… system consisting of (1) a NADH-specific FAD flavoprotein reductase, (2) a Fe2S*2 Cys4 iron-sulfur redoxin termed putidaredoxin (Pd) and (3) cytochrome P450cam, abbreviated cytochrome m for monoxygenase. The interaction of Pd and cytochrome m in the overall monoxygenase reaction is studied by …
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Mechanistic Studies and Inhibition of N-hydroxylating Monooxygenases
… monooxygenases (NMO) are members the class B flavoprotein monooxygenases. They catalyze the N-hydroxylation of lysine and ornithine and play and essential role in the biosynthesis of hydroxamate containing siderophores. Siderophores are high affinity iron-chelators composed of catechol and …
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Advancing Dynamic Imaging for Microfluidic Stimulated Tissues: Biology, Techniques, and Applications
… redox-associated changes in electron transfer flavoprotein (ETF) autofluorescence to fatty acid oxidation in living pancreatic islets. We found that elevated glucose (>10 mM) decreases the ETF redox state within 20 minutes in islets when presented with a mixed nutrient condition associated with …
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Regulation of nitrogen fixation in <i>Klebsiella pneumoniae</i>: The role of Fnr in oxygen signal-transduction
… und molekularem Sauerstoff geregelt. NifL, ein Flavoprotein, das redox-sensitiv ist, scheint, das Sauerstoffsignal über eine Konformationsänderung auf NifA zu übertragen. Kürzlich konnten wir zeigen, dass in einer K. pneumoniae fnr null Mutante NifL das Signal für Sauerstofflimitierung nicht …
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Biochemical Genetic Analysis of Blue Light Responses in Neurospora Crassa
… blue light responses of Neurospora. (iii) The flavoprotein nitrate reductase was found not to be involved in the photoresponses being studied. The responses of photosuppression and phase shifting of circadian conidiation, as assayed in nitrate reductase mutants, showed no significant …
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Computational studies of cerebellar cortical circuitry
… results presented in this thesis, based on the flavoprotein autofluorescenece imaging, show that the tottering mouse dysfunction is associated with the presence of transient synchronous low frequency oscillations in the cerebellar cortex. For a large majority of cerebellar cortical neurons the …
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Factors Affecting the Heat Resistance of Clostridium perfringens Spores
… These results identified the electron transfer flavoprotein alpha chain (EtfA) as the DPA synthase of C. perfringens. Inactivating the etfA gene in C. perfringens resulted in a strain that could begin, but not complete, the sporulation process and produced dramatically lower amounts of DPA than …
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Direct Redox Regulation of F-Actin Assembly and Disassembly by MICAL
… Interestingly, the MICALs belong to a class of flavoprotein monooxygenase/hydroxylase enzymes that associate with flavin adenine dinucleotide (FAD) and use the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH) in oxidationreduction (Redox) reactions. Although MICALs have no known …
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ENZYMOLOGY AND MOLECULAR BIOLOGY OF BILE ACID 7alpha- AND 7beta- DEHYDROXYLATION BY THE INTESTINAL BACTERIA CLOSTRIDIUM SCINDENS AND CLOSTRIDIUM HYLEMONAE
… in C. scindens VPI 12708. Expression of a 62 kDa flavoprotein and reactionwith [24-14C] 3-oxo-DCA and NADP resulted in a product of greater hydrophilicity than deoxycholic acid. The identity of this product was not determined. A second gene appears to share a common evolutionary origin with the …
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Direct Redox Regulation of F-Actin Assembly and Disassembly by MICAL
… Interestingly, the MICALs belong to a class of flavoprotein monooxygenase/hydroxylase enzymes that associate with flavin adenine dinucleotide (FAD) and use the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH) in oxidationreduction (Redox) reactions. Although MICALs have no known …
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Identifizierung und Charakterisierung der Isoflavon-umsetzenden Enzyme aus dem humanen Darmbakterium Slackia isoflavoniconvertens
… Racemat. Neben dem Elektronentransfer-Flavoprotein IfcBC wurden auch die THDR, DZNR und IfcE als FAD-haltige Flavoproteine identifiziert. Zudem handelte es sich bei IfcE um ein Eisen-Schwefel-Protein. Nach Induktion der für die Daidzein-Umsetzung kodierenden Gene wurden mehrere …
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Regulation of nitrogen fixation in Klebsiella pneumoniae: NifL, one protein transducing two environmental signals to the nif-transcriptional activator NifA
… Stickstoff und Sauerstoff. NifL ist ein Flavoprotein, welches das Sauerstoffsignal wahrscheinlich durch einen redoxsensitiven Konformationswechsel transduziert. Wir haben die Redoxeigenschaften von NifL, welches heterolog in Escherichia coli in Anwesenheit von molekularem Sauerstoff und …
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Highly Selective Attentional Modulation of Task-Appropriate Neural Populations in Primary Visual Cortex
A wide variety of different forms of attention have been described in the human and non-human literature, however the recently developed Input Gain Model of visual attention proposes that a simple neural mechanism, multiplicative gain, may be employed to explain much of the available data on visual …
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Understanding the Effects of Higher-Order Assembly on the Structure of Sulfite Reductase
… holoenzyme, consisting of eight subunits of a flavoprotein (SiRFP) and four subunits of a hemoprotein (SiRHP). SiRFP is a diflavin reductase, a homolog of cytochrome P450 reductase (CPR) that is composed of the following domains: a FMN binding flavodoxin (Fld)-like-domain, a connection domain …
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