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Showing 1 to 20 of 146 for “"Flask"”.
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Upgrading alcohols with bio-oxidation and chemo-catalysis in single-flask processes
… with chemo-catalytic transformations in single-flask processes. In this vein, our group targets alcohol substrates for upgrading with biocompatible chemistry. Alcohols are attractive targets for their easy access from renewable feedstocks yet scarce opportunities for upgrading in water. Since …
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Preliminary investigation of growth and antimicrobial production by streptomyces polyantibioticus : from shake flask to stirred tank bioreactor
… from submerged cultures of SPRT in shake flasks, and scale-up of the process into a laboratory stirred tank bioreactor (STR) was done in the present study. The work focused on obtaining growth kinetics and suitable operating conditions for cultivation. Characterisation of the growth …
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The process intensification of biological hydrogen production by Escherichia coli HD701
… HD701 using a two‐phase process (growth in shake flasks, followed by hydrogen production within a bioreactor). Ideally a commercial process would need to be in a single vessel (bioreactor), which therefore resulted in this investigation of the scale‐up of twophase fermentations to 5 L stirred tank …
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Decomposition of Urban CO2 Fluxes into Fossil and Biogenic Components using a novel Relaxed Eddy Accumulation System
… and that concentration differences between the flask pairs agree with the differences of in situ measurements within (0.02 +- 0.21) ppm. In total, 300 flask pairs were collected, of which 95 were analyzed for 14CO2. These were grouped and interpreted according to atmospheric sampling conditions. …
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Strained Metallocenophanes: Potential Monomers for Metallopolymers
… Sp,Sp-115 through recrystallization and flask-to-flask condensation were unsuccessful. In the next step, the preparation of the phospha[1]ruthenocenophane Sp,Sp-166 was attempted. Following a similar procedure that was applied for the synthesis of the sila[1]ruthenocenophane Sp,Sp-115, …
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Organochlorine Pesticide Residue in Fish Fillets From Lake Springfield, Missouri
… solution was then transferred to a round bottom flask and reduced to 3 mL by rotary evaporation and transferred to a 5 mL volumetric flask and 0.5 ppm of hexachlorobenzene was added for an internal standard. The solution was diluted to the mark and the organochlorine pesticide residues were …
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Production of Polyhydroxyalkanoates by Pseudomonas mendocina using vegetable oils and their characterisation
… source. The fermentation profiles at shaken flask and bioreactor levels revealed that vegetable oils supported the growth of Pseudomonas mendocina and PHA accumulation in this organism. Moreover, when vegetable oil (coconut oil) was used as the sole carbon source, fermentation profiles showed …
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Poly(3-Hydroxybutyrate), P(3HB) production and its biomedical applications
… Different modes of fermentation including shaken flask, batch, fedbatch and two-stage fermentation were investigated in the study. A modified G-medium was formulated and used throughout the study. Potassium and sulphate were identified as the main limiting factor for P(3HB) accumulation in …
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Improvement of engineered thermostable xylanase production via immobilization of recombinant escherichia coli onto graphene oxide
… (20°C, 0.5 mM, IPTG and pH 7) using shake flask cultivation followed by bioreactor conditions (20°C, 0.5 mM, IPTG and pH 7) in batch fermentation. The immobilized cells onto GO using shake flask cultivation exhibit about 3.8-fold increase in xylanase production (0.4821 U/ml) or equal to …
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The development of an industrial process to produce AC γ-linolenic acid using Choanephora cucurbitarum
… fungal strains were screened under shake flask conditions. The major criteria used in evaluating these strains were, the yield of γLA per unit volume (g/l) and γLA as a percentage of fatty acids, which is important in the downstream processing of γLA . Other parameters of industrial …
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Factors affecting variability in anther culture and in regeneration of androgenic embryos of Solanum phureja
… the anthers were cultured in two groups of five flasks. In the first group, each flask contained the 30 anthers from 6 buds; the second group, each flask contained 1 anther from each of 30 buds -- a total of 30 anthers per flask. Significantly smaller coefficients of variation were observed for …
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Overproduction of xylose isomerase in recombinant Escherichia coli
… and inducible enzyme activity in shake flask cultures. E. coli strain LE392 (pRK248/pTXI-l) performed the best with respect to nearly all of the parameters tested. The stability of the overproducing plasmids was tested in prolonged serial shake flask cultures. Segregational instability …
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Hydrodynamics, dissolution, and mass transfer effects in different dissolution testing apparatuses and laboratory systems
Dissolution testing apparatuses and shaker flasks agitated by shaker tables are laboratory systems routinely found in many laboratories at most companies and agencies, and especially in pharmaceutical companies. These devices are commonly used in a number of applications, from drug development to …
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Cultivation of Palmaria palmata: spore release, vegetative propagation and decontamination techniques.
… These experiments were subsequently up-scaled to flask culture and at-sea experiments. The main observation from the spore experiments was that an oomycete infection is highly prevalent and limits the production of this species. However, resistant spores were identified which have potential to be …
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Characterizing resistance in flue-cured tobacco to Globodera tabacum solanacearum
… with increased temperature. More swollen and flask shaped nematodes developed in roots of K 326 than those of NC 567. Resistance reduced development of vermiform juveniles to swollen nematodes in NC 567. However, development of swollen nematodes to flask shaped nematodes was similar for both …
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Bioremediation of Effluent Water in a Coal Ash Disposal Area: Optimizing Microbial Sulfate Reduction
… reducing bacteria (SRB) mechanisms within flask wetland microcosms, simulating conditions comparable to the final pond at the disposal area. The effectiveness of acetic acid, lactic acid, mushroom compost, and cow manure were analyzed through a series of laboratory experiments treating …
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The chlorination of sulfate lignin
… lignin was chlorinated in two liter wolff flask which was equipped with a motor driven stirrer, and the chlorine flow was measured by a calibrated U-tube differential gage. The flask was charged with the alcohol-lignin mixture and chlorinated from one to seven hours. The rates of chlorine …
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Bioreactor design for scaleup of Catharanthus roseus hairy root cultures for production of indole alkaloids
… (ii) Scaleup of hairy root cultures from shake flasks to bioreactors. An extraction method was adapted for the rapid quantification of C. roseus hairy root extracts, and was used for quantifying alkaloid yields from five different root clones. Optimum analytical wavelengths were chosen for …
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Copper supplementation and monocyte function in growing beef calves
… techniques, immunomagnetic (IM) and culture flask adherence (CF), were compared for isolation of a viable, homogeneous monocyte population. The CF technique for monocyte isolation resulted in both a greater number of cells exhibiting phagocytic activity, as well as, an increased phagocytic …
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Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production
… produced with high levels of expression in shake-flask culture. Furthermore, preliminary studies have also shown that the molecular weight of the amidase was approximately 35kDa, and that it acts optimally at a temperature and pH of 50°C and 7.2 respectively.
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