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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 49 for “"Flagellin"”.
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A Flagellin-Poxvirus Antigen Vaccine: Strengths and Limitations
Bacterial flagellin is a potent adjuvant that enhances adaptive immune responses to a variety of antigens. The vaccinia virus antigens L1R and B5R are highly immunogenic in the context of the parent virus, but recombinant forms of the proteins are only weakly immunogenic. Therefore, we evaluated …
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Molecular basis of NAIP/NLRC4 inflammasome activation by flagellin
… determine the molecular mechanisms by which the flagellin gene from Salmonella enterica serovar Typhimurium (S. Typhimurium) activates the NAIP/NLRC4 inflammasome and its contribution to the host protective immune response against salmonellosis. Inflammasomes are multi-protein complexes formed in …
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Development of a flagellin-based multivalent vaccine against Pseudomonas aeruginosa
… vaccine containing P. aeruginosa type A and B flagellins as well as the outer membrane proteins OprF and OprI would promote enhanced clearance of P. aeruginosa. To test this I utilized two animal models, mice and 4-6 month old African green monkeys. Intramuscular immunization of mice with …
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The Transcriptional Regulation Of Flagellin-Induced Innate Protection Of The Cornea: Role Of Irf1 And Atf3
<p>Pre-exposure of the cornea to TLR5 ligand flagellin induces profound mucosal innate protection against infections by reprogramming gene expression. This study explored the flagellin-induced modifications of transcription factor expression and function, specifically of IRF1 and ATF3 in corneal …
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Regulation of flagellin glycosylation genes in Campylobacter jejuni: influence of NssR, the nitrosative stress response regulator.
… is necessary for Campylobacter virulence. The flagellin proteins are extensively glycosylated in C. jejuni with pseudaminic acid and related derivatives; glycosylation is required for flagella filament formation. The flagellin glycosylation locus in C. jejuni NCTC11168 comprises 50 genes, 50% …
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The development of a flagellin surface display expression system in the gram-positive bacterium, Bacillus halodurans Alk36
… endogenous genetic background to over-produce flagellin protein continuously. In order to harness this ability, key genetic tools, such as gene targeted inactivation, were developed for this strain. The hag gene which codes for flagellin was inactivated on the chromosome giving rise to the B. …
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Solid phase peptide synthesis and TLR-5 activity analysis of pertide fragments from the Salmonella muenchen flagellin protein
… could help remedy a variety of disorders. Flagellin, the primary component of bacterial flagella, is the only known TLR-5 ligand. Three short regions within this protein are suggested to activate TLR-5: Peptide-N1, LQRVRELAVQ; Peptide-N2, LAVQSANGTNSQSD; and Peptide-C1, QNRFNSAITNLGNT. …
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Identification of the Polar Flagellum Genes in Azospirillum brasilense
… As-Fla, specific for polar flagellum, the flagellin sizes for mutants and the wild type were determined. Some of the mutants had 96-kDa flagellin like the wild type while others had truncated flagellin as small as 65-kDa. Our hypothesis is that mutants with reduced size flagellin do not …
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Determining the Innate and Adaptive Immune Responses to Vesicular Stomatitis Virus Vaccine Vectors
… the addition of a vaccine adjuvant, bacterial flagellin, expressed intracellularly from the viral genome (M51R-F). The ability of the vectors to induce innate immune responses was tested in murine dendritic cells. The presence of flagellin led to production of IL-1beta; and an inflammatory form …
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Rough leucine auxotrophic strains of <i>Brucella</i> expressing <i>Salmonella</i> flagellin C conjugated gonadotropins, an immunocontraceptive brucellosis vaccine for feral swine population control
Brucellosis, caused by Gram-negative bacteria of the genus <i>Brucella</i>, is a zoonotic disease with global impacts on human, livestock, and wildlife health. Around 500,000 cases of human brucellosis are reported by the World Health Organization annually. Even though brucellosis has been …
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Assessment of endosomes as sites of signal transduction in plants during bacterial attack
… in response to detection of the bacterial PAMP flagellin and the corresponding proteome changes occurring in endosomes following detection of bacteria as part of defence responses. To understand and test the role of endosomes in defence characterised the proteomes of several endomembrane …
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Dissection of Innate Immunity in Tomato and Tolerance to Bacterial Wilt in Solanaceae species
… receptors (PRRs). The best-studied LRR PRR is Flagellin-Sensitive 2 (Fls2), the receptor of a 22-amino acid long epitope of bacterial flagellin, called flg22. In this project, alleles of FLS2 of different tomato cultivars were sequenced and compared to each other to get insight into natural …
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Immunomodulatory effects of LL-37 in the epithelia
… in response to IL-1β and the TLR5 agonist flagellin, and synergistically increased IL-8 production by bronchial epithelial cells in response to IL-1β, flagellin, and the TLR2/1 agonist PAM3CSK4. Treatment of bronchial epithelial cells with LL-37 and the TLR3 agonist poly(I:C) resulted in …
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Functional genomics of motile commensal intestinal bacteria
… innate immune system has evolved to recognise flagellin, (the major protein component of the bacterial flagellar filament). Flagellate microbes can potentially stimulate the immune systems of mammals, and thus have significant immunomodulatory potential. The flagellum-biogenesis genotype and …
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Characterisation of potential regulators of PAMP-triggered immunity
… recognise the bacterial elongation factor Tu and flagellin, respectively. A variety of downstream responses upon PAMP perception have been described. However, it is still poorly understood how EFR- and FLS2-dependent signalling is mediated. Here, I used two different approaches in order to …
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Vývoj vybraných CD znaků a jejich role ve fylogenezi imunitního systému člověka
… significant similarity to the archaebacterial flagellin domain was found. This flagellin sequence in the CD38 avian molecule is located in the region of transmembrane domain, indicating that the occurrence of this sequence might be related to the formation of the transmembrane domain. The …
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Chlorid, ein neues Signalmolekül für Bakterien
… in H. halophilus Cl--abhängig ist. Das Flagellin wurde gereinigt, und es wurde ein spezifisches Antiserum dagegen hergestellt. 4. Immunologische Analysen ergaben, daß die Synthese des Flagellins Wachstumsphasen-abhängig war. In der log-Phase und in der frühen stationären Phase wurden …
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Molecular Analysis of Roseburia Cecicola, a Motile Anaerobe, as a Foundation for Study of Bacterial Motility and Chemotaxis in the Murine Gastrointestinal Ecosystem
… in the mouse cecum. I purified the main protein (flagellin) of the flagellar apparatus of the organism. The purified protein was then used in raising polyclonal and monoclonal antibodies. In addition, the gene encoding the protein was cloned and sequenced.
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Theoretical and computational investigations into structure-function relationships of biomolecular machines at atomic resolution
… process. The building blocks of flagella, flagellin monomers, are pumped by a type III secretion system, through the flagellar interior channel to the growing tip. After a flagellin monomer binds to the tip of the filament, the growing flagellum is extended. The flagellin translocation …
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Characterising the immune response to Salmonella and Salmonella surface antigens during a systemic infection
… have been examined. Immunisation with soluble flagellin (sFliC) induces a potent Th2 response. Despite this, immunisation with sFliC results in accelerated clearance of STm after the first week of infection in an antibody independent, but T-bet-regulated manner. This suggests that the Th2 …
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