Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 53 for “"Filopodia"”.
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Purinergic Signaling Regulates Filopodia-Induced Zippering
… cell-cell adhesion in carcinoma cells by filopodia-induced zippering. Adenosine-generating ecto-enzyme, CD73, was down-regulated in moderately- and poorly-differentiated, invasive, and metastatic endometrial carcinomas. CD73 expression and enzyme activity in normal endometrium and …
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Regulation of filopodia dynamics is critical for proper synapse formation
… of spiny synapses has been thought to occur via filopodia shortening followed by the recruitment of proper postsynaptic proteins, however the precise function of filopodia remains controversial. Thus the goal of this study was to investigate the dynamics of dendritic filopodia and determine their …
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Self-assembly of filopodia-like structures on supported lipid bilayers
Filopodia are finger-like protrusive structures of cells, comprised of actin bundles, which can serve as sensory organelles. To probe their pathway of assembly we have reconstituted filopodia-like structures (FLSs) by applying frog egg extracts to supported lipid bilayers containing …
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Antibody technology identifies distinct mechanisms controlling the lengths of filopodia-like structures
Filopodia are finger-like cytoplasmic protrusions that contain filamentous actin and extend beyond the leading edge of migrating cells. Filopodia are thought to facilitate both sensing of signaling molecules and the movement of cells towards them. Profuse filopodia formation is seen in metastatic …
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miR-125b toggles dynamics and structure of dendritic filopodia in developing hippocampal neurons
… responding to external cues sensed by dendritic filopodia, they participate in the key decision-making processes in developing dendrites: where and how to grow. This study focuses on the role of miR-125b, a brain abundant microRNA, for its role in the dynamics and structure of filopodia in …
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The role of Ena/VASP and associated proteins in regulation of neuronal morphology and filopodia architecture
… an inability to form bundled actin filaments and filopodia. In addition, splice isoforms Mena, a member of the Ena/VASP family, are tightly regulated during cancer metastasis and we determined these splicing changes influence the assembly of actin protrusions. My findings have helped to elucidate …
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Neuronal filopodia borne along tips and shafts of dendrites comprise two distinct populations as evidenced by differences in structure and dynamics
… their putative precursors – the dendritic filopodia. This set off several lines of investigation into filopodial structure and function, notable among which is the work by Portera-Cailliau et al. who showed in 2003 that growth cone filopodia differ from shaft filopodia in terms of densities …
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Linking WRP/srGAP3 to the Cognitive Deficits in 3p- Syndrome and Its Role in the Regulation of Dendritic Filopodia Formation
… via its IF-BAR domain where it is enriched in filopodia like projections. During dendritic filopodia formation, WRP functions to regulate the WAVE-1 complex and its downstream effectors, including the Arp2/3 complex. Loss of WRP in vivo and in vitro results in a reduction of dendritic spines, …
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Frazzled And Abelson Interact To Regulate The Actin Cytoskeleton In Drosophila
… processes underlying formation of filopodia, microspikes and membrane ruffles. So, we established a Drosophila S2 cell culture paradigm to study how Frazzled and Abelson may co-regulate actin-dependent morphology. Ectopic expression of Frazzled in S2 cells produces long …
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The Cytoskeletal Mechanisms of Cell-Cell Junction formation in Endothelial Cells
… maintain contact through thin bridges formed by filopodia-like protrusions connected by VE-cadherin-rich junctions. Bridges share multiple features with conventional filopodia, such as an internal actin bundle associated with fascin along the length and VASP at the tip. Strikingly, unlike …
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Toca-1 driven actin polymerisation at membranes
… of outward facing protrusions, lamellipodia and filopodia. In an in vitro system that recapitulates the formation of filopodia-like structures (FLS) on supported lipid bilayers, Toca-1 is recruited early, suggesting a Toca-1 scaffolding mechanism could precede the recruitment of other actin …
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Functional and ultrastructural investigation of mouse and human dendritic spines
… and patch-clamp electrophysiology to identify filopodia as the structural substrate for silent synapses in adult neocortex. Of 2,234 spiny synapses from adult mouse layer 5 pyramidal neurons, a surprisingly large fraction (~25%) lacked AMPA receptors. These putative silent synapses were located …
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Spatio-temporal properties of membrane-localized actin nucleating complexes
… and invaginations that drive these processes. In filopodia, which are finger-like protrusions comprised of bundled actin filaments, actin regulatory proteins are believed to assemble a distal 'tip complex' which stimulates actin nucleation at the membrane. However how these regulators collectively …
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Investigations into membrane protrusions mediated by IRSp53 IMD
… and Cdc42 in the formation of lamellipodia and filopodia, respectively. IRSp53 interacts with many other effectors in the formation of these structures, via various protein interaction domains. IRSp53 also contains an IMD (IRSp53/MIM homology domain), which is able to induce formation of …
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The rule of Myosin II and Rap2 in synaptic structure and function
… mushroom-shaped spines, and an increase in thin, filopodia-like structures. Treatment with blebbistatin, a chemical inhibitor of myosin II, phenocopied this result. Live imaging revealed that mature spines unravel into filopodia within tens of minutes of myosin II inhibition by blebbistatin. …
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Dendritic Cell Migration and Traction Force Generation in Engineered Microenvironments
… traction stresses at the leading edge or filopodia. We illustrate that spatiotemporal pattern of traction stresses can be used to predict changes in the direction of DC motion. Additionally, we determine the characteristic duration of local dendritic cell traction forces and correlate this …
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The role of the extracellular matrix in neuronal development and perinatal brain injury
… with expression of HA and HASs on lamellipodia, filopodia, immature neurites, differentiated processes, and synapses (Chapter 3). Notably, HA was expressed earlier and more widely than PNNs. Neurons also expressed Hyal1–3 mRNA. In Chapter 4, pharmacological inhibition of HAS activity with 4-MU …
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Regulatory mechanisms of MYO6 motor domain activity and tail interactions
… engineered MYO6 plus-end directed mutant forms filopodia on the cell surface, which upon expression of the phosphomimetic significantly increases in number. Co-expression of the phosphomimetic and wildtype MYO6 causes a re-distribution of their localization to the filopodia tip and stalk, …
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Evaluating the effects of acoustic stimulation on fibroblast cell migration and muscle fibre formation.
… particularly the formation of lamellipodia and filopodia. Overall lamellipodia was the most prominent actin structure displayed by the lung cell (LL24), whereas filopodia was the most prominent actin feature displayed by the fibroblast derived from subcutaneous areolar/adipose tissue. Vinculin …
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P21- ACTIVATED KINASE 4 AND ACTIN CYTOSKELETON DYNAMICS
… THE FORMATION OF ACTIN-RICH STRUCTURES SUCH AS FILOPODIA IN MAMMALIAN CELLS. PAK4 PHOSPHORYLATES LIM KINASE (LIMK), WHICH IN TURN, PHOSPHORYLATES THE ACTIN DEPOLYMERISATION FACTOR, COFILIN. COFILIN PHOSPHORYLATION RESULTS IN THE STABILISATION OF THE FILAMENTOUS FORM OF ACTIN. THE PRIMARY GOALS …
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