Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"Filamentous Actin"”.
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A study on the modulation of alpha-actinin and filamentous actin
Cells use actin to provide structure and stability to the membrane, to drive motility, and to adhere to, pull on, and reorganize the extracellular matrix. Here I demonstrate that α-actinin-4, a well-known actin crosslinking protein and long believed to be the primary actin bundling protein for …
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Combined optical trapping and single molecule fluorescence to study the force-dependent binding kinetics between filamentous actin and its partners
Actin filaments are a major constituent of the cytoskeleton in most eukaryotic cells. They function as a connection between the cell body to the focal adhesions in order to transmit forces into and out of the cell. During the force transduction process, many proteins bind to actin filaments in …
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Soft matter in motion: from actin to active emulsions
… a range of soft material systems, including filamentous actin, liquid crystal droplet and block copolymer to study their dynamics under equilibrium and non-equilibrium conditions. By following the motion of filamentous actin under a fluorescence microscope, we directly observed a …
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The SAC1p, an integral membrane protein involved in secretory pathway function and actin function in Saccharomyces cerevisiae
… results in the selective growth of the bud. The actin cytoskeleton has been implicated as the mediator of the polarized mode of yeast cell growth. The filamentous actin cytoskeleton consists of two structures, asymmetrically-arranged cortical patches and cables which are aligned along the mother …
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Regulation of Homophilic and Heterophilic Adhesion Among Classical Cadherins
… imaging assessed cadherin clustering and actin organization in E-cadherin expressing cells cultured on E-, N-, and C-cadherin ectodomains. As expected, the formation of filamentous actin correlated with the Rac activation amplitudes in E-cadherin expressing cells. The results also showed …
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The Role of EHD2 in Endothelial Cells
… This hypothesis is supported by the loss of filamentous actin and an increase in serrated junctions in cells that have an excess of EHD2. This change is not just seen in cells - when EHD2 is knocked out in fish, there are visible changes in the junctions that are similar to what occurs in the …
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Src Kinase Regulates TGFβ And Hyaluronan Induced Epicardial Cell Invasion, Differentiation And Migration
… as well as TGFβ2-induced EMT, cell invasion, and filamentous actin polymerization. Src activity is sufficient to drive epicardial activation of EMT, but not vascular smooth muscle differentiation. These data show that Src is required in the context of TGFβ2-stimulated invasion and differentiation, …
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The role of tunneling nanotubes in mediating intercellular communication in cancer
… (50-800 nm in diameter), long, non-adherent filamentous actin-based protrusions of the plasma membrane that can serve as intercellular bridges. They can aid in the sharing of proteins, genes, and other cargo between cells. In cancer, TNTs are particularly prevalent and have been shown to …
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Mapping the actin and actin binding proteins interactions : from micromechanics to single molecule force spectroscopy
… building models describing cell behavior. Filamentous actin (F-actin), as one of the major constituents of the cytoskeleton, has been the target of extensive in vitro studies to determine its mechanical properties in bulk. However, there is still a lack in the understanding of how the …
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Alteration of prenylation: Effect on neurite outgrowth and Rho GTPase signaling
… cofilin phosphorylation with effects on actin filament content since cofilin is an actin depolymerizing agent. We use western blot analyses and immunocytochemistry to assess phosphorylated and total cofilin and find no significant deviations from control conditions. However, the amount of …
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alpha-Actinin-4 and the podocyte: Implications for focal segmental glomerulosclerosis
… Mutations in the ACTN4 gene, encoding an actin-crosslinking protein, are causative of late-onset familial FSGS in humans. We have developed a mouse model of FSGS by expressing a high-affinity variant of alpha-actinin-4 (K256E) in a podocyte-specific manner. Transgenic mice display podocyte …
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Targeting the actin cytoskeleton in HER2+ metastatic cancer cells using a macrolide toxin
… by cytoskeletal rearrangements that produce filamentous actin (F-actin) based structures that penetrate and degrade extracellular matrix to facilitate tumour invasion. Advancements in targeted therapy have made F-actin an attractive target for the development of new cancer therapies. In this …
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Understanding Actin Dynamics and Its Regulatory Mechanisms in Developing Stereocilia
Stereocilia, the actin-based protrusions of auditory hair cells, are essential for converting mechanical stimuli into electrical signals. Their formation and maintenance require tightly regulated actin dynamics, including elongation and widening through filamentous actin (F-actin) incorporation. …
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Biochemical and genetic analysis of the adaptor protein SH3P7 : insights from a newly generated knockout mouse
SH3P7 (mAbp1/Hip55) is a widely expressed actin-binding adaptor protein identified as a substrate for protein tyrosine kinase (PTK) after BCR activation. In addition to two protein-binding modules specific for filamentous actin, SH3P7 also contains an SH3 domain. The biochemical studies presented …
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The Interaction of Cofilin with the Actin Filament
The regulation of filamentous actin: F-actin) production from the polymerization of globular actin: G-actin) within the cell is critical for many cell functions. Since actin is found in all cells, understanding how actin-binding-proteins: ABPs) bind and how their regulating mechanisms work is not …
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Molecular signaling programs underlying neutrophil polarity and chemotaxis
… fully understood is the mechanism that governs actin cytoskeleton dynamics and assembly in neutrophils. The asymmetric accumulation of filamentous actin (F-actin) at the leading edge provides the driving force for protrusion and is essential for the development and maintenance of neutrophil …
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Effector-driven actin reorganisation restricts Salmonella invasion.
… into the cytosol of the cell and manipulate the actin cytoskeleton, driving pathogen entry. Two of the delivered effectors, namely SopE and SopE2, activate host Rho GTPases and subsequently the Arp2/3 complex, which results in actin reorganisation. Whereas the role of SopE in promoting bacterial …
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Investigating the Structure of the Papain-Inhibitor Complex using SPR and NMR
… this method to the study of another system: filamentous actin (f-actin) and the actin-binding domain of abelson tyrosine-protein kinase (ABL2-FABD). The creation of nanopores on an AFM sensor chip surface was successful. ICP monomers bound selectively into the pores. Attempts to form the …
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Diabetes and the Female Heart
… ryanodine receptor type 2 (RyR2) clusters, and filamentous actin (F-actin). Results showed that while diabetic rats developed systemic dysfunction including glucose intolerance and alterations in plasma biomarkers, myocardial energetics were preserved at the trabecula level. Baseline comparisons …
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Antibody technology identifies distinct mechanisms controlling the lengths of filopodia-like structures
… finger-like cytoplasmic protrusions that contain filamentous actin and extend beyond the leading edge of migrating cells. Filopodia are thought to facilitate both sensing of signaling molecules and the movement of cells towards them. Profuse filopodia formation is seen in metastatic cancer cells …
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