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Showing 1 to 20 of 32 for “"Ficoll"”.
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Permeation of ficoll and ficoll sulfate through glomeruler basement membrane : effects of molecular size and charge
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1998.
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Dynamics of the glomerular filtration barrier - Physiological and pathophysiological aspects
… chromatography (HPSEC) detection of FITC-Ficoll in serum and urine. This permeability model is extremely sensitive, and by using HPSEC, it was possible to measure extremely low concentrations of Ficoll molecules in the urine. Under resting conditions the sieving coefficient (θ; …
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Effects of plasma proteins on the sieving of macromolecular tracers in the kidney
… that the sieving coefficients of the tracers Ficoll and Ficoll sulfate across isolated GBM were greatly elevated when BSA was present at physiological levels (Bolton et al. 1998). It was suggested that most of this increase was the result of steric interactions between BSA and the tracers …
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Studies of the idiotypic network through the use of a novel hemolytic plaque assay
… response following murine immunization with DNP-Ficoll was analyzed using such an assay, peak plaque forming cell (PFC) numbers were found to coincide with peak Ab1 PFC numbers in both the primary and secondary response. In addition, this Ab2 response was found to be T independent. The murine …
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SYNTHESIS AND STUDY OF ANTI-TUMOR VACCINES
… mice were immunized and boosted with a rhamnose-Ficoll (Rha-Ficoll) conjugate formulated with alum as adjuvant to generate the appropriate concentration of anti-Rha antibodies in the mice. Anti-Rha antibody titers were >25-fold higher in the groups of mice immunized with the Rha-Ficoll conjugate …
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Biophysical Analyses of Intermolecular Ion-Pairs in Protein-DNA Complexes
… kinetic parameters of the Antp homeodomain using Ficoll, as a macromolecular crowder, to investigate the role of ion-pair dynamics in DNA target search. Using fluorescence-based stopped flow kinetics experiments, we show an increase in sliding length for the Antp homeodomain in the presence of …
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Hindered transport in composite hydrogels
… serum albumin) and three narrow fractions of Ficoll, a spherical polysaccharide, were used. For the partitioning and convection studies, four narrow fractions of Ficoll were used. Diffusivities of fluorescein-labeled macromolecules were measured in dilute aqueous solution (D[infinity]), …
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Mechanisms of Immunosuppression in Murine Malaria
… substance did not reduce the PFC response to DNP-Ficoll, a T-independent antigen, but it was capable of reducing the T-dependent anti-DNP and anti-SRBC PFC responses in high dilution. The data obtained from these experiments suggested that B cells are not the target of suppression.
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Early divergent B cell differentiation during antibody responses
… (NP), were immunized with NP-Ficoll. Varying the antigen dose altered the proportion of B cells that entered the extra follicular or GC responses and the amount of class switch recombination (CSR). The expression of phenotypic markers and switched antibody protein in parallel …
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Probing the sensitivity of autoantibody production to B cell depletion by Rituximab
… response of QM mice to immunisation with NP-Ficoll, concurrent infection with attenuated Salmonella enterica serovar Typhimurium increases splenic capacity to support plasma cells. The enhanced support may reflect locally increased IL-6 production.
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Diseño de un sistema de dos fases acuoso para la recuperación de células madre a partir de tejido adiposo y escalamiento en biorreactor para su producción y uso en terapias regenerativas alogénicas
… ATPS 5,6% PEG 8.000 – 7,5% DEX 500.000, 13.6% Ficoll 400,000 – 11.6% DEX 75,000 y 10% Ucon 5.100 – 10% DEX 75.000 para la purificación de distintas muestras de ASC, mientras que para escalar la producción en cultivos en suspensión se testearon los microcarriers Cytodex 1 y Cultisphere G, además …
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Nonspecific Cell-Mediated Cytotoxicity in the Cat
… from blood and spleen were purified over Ficoll-Hypaque and added to uninfected, vaccinia-infected, or herpes-infected adherent feline origin target cells and to a lymphoid tumor cell line, FL74, which grows in suspension. Natural cytotoxic (NC) cells, which lyse adherent targets, were …
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Characterization of Dendritic Cells in the Bovine Mammary Gland
… Cell cultures contained digested and Ficoll separated mammary tissue or supramammary lymph node tissue. While the presence of CCR7 and CD80 was confirmed, CD80 and CCR7 transcripts were not upregulated after LPS stimulation. Further, CD11c, CD14, MHCII, CD11b, CD11a, and CD205 protein …
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A T- és B-limfocita alpopulációk eloszlásának vizsgálata endometriózisos betegekben
… vizsgáltuk. A perifériás mononukleáris sejteket ficoll gradiens technikával izoláltuk, majd immunfenotipizálást végeztünk és áramlási citometriás mérés keretében meghatároztuk a follikuláris helper T-limfocita (Tfh) alcsoportok, a follikuláris regulatorikus T-limfociták (Tfr), valamint a …
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Inhibition of Mitochondrial Translation as a Therapeutic Strategy for Acute Myeloid Leukemia
… Primary AML mononuclear cells were isolated by Ficoll centrifugation from peripheral blood. Flow cytometry dye; JC-1, Carboxy-H2DCFDA, Mitotracker GreenFM. Leukemia stem cell activity was assayed by human AML engraftment in NOD/SCID mice. Results: TIG induced cell death in primary AML patient …
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Producción de Intermediarios Reactivos de Nitrógeno en cultivo de células mononucleares de cabras expuestas al BCG de M. bovis, suplementadas con 1,25 Dihidroxivitamina D3
… las células mononucleares por el método Ficoll-Hypaque. Los RNI, nitritos y nitratos (NO2- y NO3-) fueron cuantificados por ELISA. En el tratamiento, vacuna BCG de M. bovis, la concentración fluctuó entre 10.84 y 17.06 mM/l. Con la 1,25(OH)2 D3, entre 13.93 a 25.38 mM/l. En el combinado, …
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Effects of macromolecular crowding and small ions on the folding, structure, and stability of Desulfovibrio desufuricans flavodoxin
… along with synthetic crowding agents (e.g., Ficoll and dextran), I have found that macromolecular crowding increases the secondary structural content of folded flavodoxin (toward that found in the crystal structure), increases flavodoxin thermal stability, and affects both the accumulation of …
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Καλλιέργεια λεμφοκυττάρων ευαισθητοποιημένων στον εχινόκοκκο ατόμων παρουσία προϊόντων υδατιδικής κύστεως: Βλαστική μεταμόρφωση λεμφοκυττάρων και δράση επί των σκωληκοκεφαλών
… mainly lymphocytes, were isolated by the Ficoll-Hypaque system and cultivaled in RPMI-1640. The immune response of the lymphocytes was studied by adding in the culture medium hydatid fluid and measuring the [³H] thymidine incorporation into the DNA of the cells. The viability of scolices …
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Vitrification of Bovine Oocytes
… concentrations of ethylene glycol (EG), Ficoll-70 and sucrose in phosphate buffered saline (PBS) for vitrification on nylon mesh and plunged into liquid nitrogen. Warming resulted in 81.7% morphological survival. The rate of blastocyst formation was 59.9% for vitrified zygotes but, this …
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Charakterisierung der allergischen Immunantwort : Vergleich der in-vitro Zytokinproduktion isolierter mononukleärer Zellen und der in-vitro Zytokinproduktion aus Vollblutproben bei Neonaten und Erwachsenen
… MCs wurden mittels der Dichtezentrifugation nach Ficoll separiert und anschließend mit PHA bzw. BLG (PHA 20 µg/ml, BLG 25 µg/ml) inkubiert. Aus den Zellüberständen wurden die Konzentrationen der Zytokine IFN-g, IL-10 und IL-13 mittels ELISA quantifiziert. <br>Bei den Neonaten wurden sowohl nach …
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