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Showing 1 to 20 of 231 for “"Fibronectin"”.
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Fibronectin domain engineering
… applications. The tenth type III domain of human fibronectin provides a small, stable, single-domain, cysteine-free protein scaffold upon which molecular recognition capability can be engineered. In the current work, we provide substantial improvements in each phase of protein engineering through …
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The importance of the tissue transglutaminase-fibronectin heterocomplex in the RGD-independent cell adhesion and fibronectin matrix deposition
… (TG-FN) with the abundant matrix protein fibronectin (FN). A further cellular response elicited after tissue damage is that of matrix remodelling leading to the release of the Arg-Gly-Asp (RGD) containing matrix fragments by matrix matelloproteinases (MMPs). These peptides are able to …
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Studies on the Structure of Human Fibronectin
<p>Plasma fibronectin is a glycoprotein that consists of two polypeptide chains of approximately 250,000 and 245,000 daltons, joined by disulfide bonds located near one end of the molecule. Fibronectin was isolated from human plasma by gelatin-agarose affinity chromatography and DEAE-cellulose ion …
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Generating single-domain antibodies against fibronectin splice variants
… antibodies which bind to splice variants of fibronectin containing EIIIA or EIIIB. Alpacas were immunized with either purified antigen cocktails, or from the ECM of tumor samples, and antibody libraries were generated. Using these libraries to pan against, I selected for VHH which bind to …
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Deformation mapping of fibronectin fibrils using digital image correlation
… mineral is deposited. Prior work has shown that fibronectin fibrils are one of the earliest ECM proteins to be assembled. Time lapse imaging techniques show that the deposition of fibronectin by osteoblasts is a highly dynamic process, in which the forming fibronectin fibril network is …
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Biochemical and mechanical cues tune fibronectin conformation and function
… including development and disease progression. Fibronectin fibers are a prevalent component of the extracellular matrix that are believed to adopt a wide array conformations with different functions. Two factors that are hypothesized to regulate fibronectin conformation, and hence fibronectin …
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Fibronectin splice isoforms in wound healing and vascular development
Fibronectins are a large family of extracellular matrix proteins that regulate cell adhesion, migration, differentiation and survival. Different isoforms of fibronectin arise via alternative splicing of the transcript of a single gene. Although many of the general functions of fibronectins have …
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Regulation of alternative splicing in the rat fibronectin gene
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1994.
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Structural and functional studies of intrinsically disordered fibronectin-binding proteins
Bacterial fibronectin-binding proteins (FnBPs) mediate adhesion of bacteria to host tissues through binding to the human protein fibronectin (Fn). FnBPs are predicted to contain a series of intrinsically disordered Fn-binding repeats (FnBRs), which undergo a disorder-to-order transition on binding …
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The Staphylococcus Pseudintermedius Adhesin Spsd Contains A Central Fibronectin-Binding Domain
… heterologous host <em>Lactococcus lactis</em> to fibronectin. This work has identified a centrally-located fibronectin binding domain in SpsD which binds the 30 kDa N-terminal domain of fibronectin with high affinity. The data indicate that a tandem β-zipper mechanism of binding may be taking …
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Strain Estimation of Fibronectin Fibrils Using Two Dimensional Digital Image Correlation
… matrix (ECM) which supports cells and tissues. Fibronectin is a non-collagenous protein produced by bone cells and is one of the earliest ECM proteins to be assembled. Fibronectin supports the assembly of several other bone matrix proteins, therefore understanding the dynamic process by which …
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Fibronectin peptides and murine embryonic stem cells : an in vitro study
… in them being referred to as pluripotent. Fibronectin is involved in a number of different functions including differentiation, growth, migration and the adhesion of cells. In addition it is well known to interact with the extracellular matrix. Fibronectin also has an essential role to play …
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Non-enzymatic glycation modifies binding of fibronectin to arteriolar smooth muscle cells /
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.]
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Cellular therapy for chronic heart failure: a key role for epicardial fibronectin
… to be underpinned by epicardial-secreted fibronectin. Thus, hESC-combination cell therapy holds clinical promise for ‘remuscularising’ chronically infarcted hearts.
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Cyclic loading of fibronectin coated beads induces differentiation in mouse embryonic stem cells
… to apply oscillating force to mES cells through fibronectin (FN) coated magnetic microbeads. The ligand coated magnetic beads were allowed to bind to undifferentiated mES cells plated on 0.6 kPa polyacrylamide gels prior to loading. The cells used were stably transfected with green fluorescent …
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Alternatively spliced isoforms of Fibronectin, Tenascin-R and other potential players in early vasculogenesis
… absence of both the EIIIA and EIIIB domains of fibronectin (FN) has been shown to negatively affect blood vessel formation and maintenance. Vascular defects have been observed in the yolk sacs of EIIIA/B double-null embryos by as early as embryonic day E9.5, and these defects are likely due to …
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Monocyte adherence to fibronectin: role of CD11/CD18 integrins and relationship to other monocyte functions
… subjects to a representative matrix component (fibronectin) were compared. Spontaneous adherence of monocytes from the control subjects was 20 to 25%, whereas that of the patients' cells was 2 to 3-fold higher and correlated with the severity of airway inflammation. Endotoxin (LPS) and cytokines …
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Modulation of Endothelial Cell Adhesion to Synthetic Vascular Grafts Using Biotinylated Fibronectin in a Dual Ligand Protein System
… retention, and native functionality compared to fibronectin alone. Initially, two SVG surrogates were identified through characterization by x-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and 125I radiolabeling. The first modification to the DL system involved direct …
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