Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"Fibroin"”.
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Characterization of the large diameter fibers in egg case silk : identification of a core fibroin, TuSp1, and localization of fibroin-like molecules, ECPs, from the black widow spider, latrodectus hesperus
… properties. At the time of these studies, fibroin eDNA sequences that encode egg case silk had not been reported in the literature. This study used conventional nucleic acid-nucleic acid screening of a eDNA library to isolate a novel gene, named tubuliform spidroin 1 , from the black widow …
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Characterization of a family of cysteine rich proteins and development of a MaSp1 derived miniature fibroin
… applications. In these studies, we develop a minifibroin for expression studies in bacteria. Using recombinant DNA methodology and protein expression studies, we develop a natural minifibroin that contains the highly conserved N- and C-terminal domains, along with several internal block repeats of …
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Template-directed Assembly of Silk in Advanced Materials for Food Security
… termed templated crystallization. Using silk fibroin as an example, templated crystallization refers to the employment of organic templates (specifically, ordered peptide seeds) to drive a phase transformation of silk molecules from disordered to ordered conformations, followed by directed …
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NOVEL NANOFIBER-BASED GRAFT FOR HEART VALVE REPLACEMENT
… I collagen protein with mechanical robust silk fibroin, and hemocompatible synthetic elastic polymer poly(glycerol-sebacate) (PGS). The central hypothesis of this project was: Electrospun grafts created from composites of type I collagen, silk fibroin, and PGS are stable, less thrombogenic and …
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Developing silica based Nanocomposites for dental applications using bombyx mori silk
… separate different silk fractions (heavy chain fibroin and light chain fibroin) from natural silk using formic acid. Silk and its fractions were regenerated for use in gelation studies and fabricating nanocomposites by adding silica. The silica was added using, hydrolysed tetraethoxy silane …
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The Synthetic spider silk fibers spun from Pyriform Spidroin 2, a glue silk protein discovered in orb-weaving spider attachment discs
… spinning of a novel attachment disc glue silk fibroin, Pyriform Spidroin 2 (PySp2), from the golden orb weaver Nephila c/avipes. MS studies support PySp2 is a constituent of the pyriform gland that is spun into attachment discs. Analysis of the PySp2 protein architecture reveals sequence …
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Direct-write assembly of 3D microperiodic scaffolds for tissue engineering applications
… in a photopolymerizable solution, (2) a silk fibroin ink, regenerated from Bombyx mori silkworm cocoons, that can be induced to change conformation in a coagulating reservoir, and (3) a hydroxyapatite (HA) particle-filled silk fibroin ink. By carefully controlling their rheological properties, …
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Self-assembling silk hydrogels for mesenchymal stem cell and drug delivery
Silk fibroin has been commonly used as sutures and surgical meshes in humans. Additionally, silk fibroin had been used for a wide range of biomedical uses including as a coating for polymer scaffolds produced for tissue engineering and silk fibroin hydrogels containing silk nanoparticles have …
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Development of a codon-optimized Latrodectus hesperus MaSp1 synthetic gene for bacterial protein expression using a seamless cloning strategy
… we developed several codon-optimized MaSp1 minifibroin constructs for recombinant protein expression in bacteria. These recombinant MaSp1 minifibroin constructs were engineered to contain the N-terminal domain (NTD), different copies of internal block repeats (ranging from 2 to 64 copies of 35 …
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FUNCTIONALIZATION OF A NOVEL ELASTOMERIC BIOMATERIAL FOR VASCULAR TISSUE ENGINEERING
… of electrospun poly(glycerol sebacate), silk fibroin, and type I collagen (PFC) has properties ideal for use as a tissue engineered scaffold for an artery graft. The long-term goal of this project was to develop a functionalized PFC scaffold for use as a small diameter vascular graft that …
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Design of Engineered Biomaterial Architectures Through Natural Silk Proteins
… of the two constituent proteins, silk fibroin and sericin. The research outlined in this dissertation focuses on the evolution in architecture and capability of silks, to effectively position a functionally-diverse, renewable class of silk materials within the rapidly expanding field of …
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Hierarchical protein assemblies with biomedical applications
… of protein-based films. Regenerated silk fibroin, a natural block copolymer with the capacity to readily re-assemble into structures with attractive physical and chemical properties that are present in native silk, was used as a protein scaffold. The regenerated silk fibroin was embedded …
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Evidence that aciniform silk and minor ampullate silk are major constituents of wrapping silk from the black widow
… My research focused on elucidating the fibroins that make up the swathing silk. Perfonning MS/MS analyses on solubilized wrapping silk fibroins digested with trypsin, we demonstrate that a novel fibroin named AcSp !-like is present within wrapping silk. Consistent with this finding, SEM …
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Příprava a charakterizace modifikovaných polysacharidových hydrogelů pro medicínské aplikace
… polysacharidu Gum Karaya a proteinu Silk Fibroin. Tento přístup umožňuje využití specifických vlastností každého jednotlivého materiálu v daném hydrogelovém systému a také studium jejich vzájemných interakcí. Při přípravě hydrogelu nebyla použita chemická síťující činidla, příprava byla …
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New water-soluble porphyrins for oxygen sensing in protein-based materials
… proteins chosen as matrices for hydrogels (silk fibroin and collagen) was performed via N-hydroxysuccinimide chemistry. Oxygen response of the complexes synthesised was tested both in aqueous solution and supported on protein-based hydrogels. Most species showed oxygen-dependent fluorescence and …
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Biomimicry of the spider silk spinning apparatus
… synthetic fibers. To accomplish this, the fibroins and natural spinning apparatus were taken apart and analyzed in order to develop a protocol that biomimics the spider's system. The laboratory procedure, using the natural process as an example, was simplified to: protein production, …
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Herstellung und Charakterisierung von Fasern aus rekombinanten Spinnenseidenproteinen und deren potentielle Applikationen
… aus eADF4-Proteinen (engineered A. diadematus fibroin 4), wobei in einem Parallelansatz zum Vergleich ein Nassspinnverfahren zur Herstellung von Mikrofasern aus wässrigen eADF3-Lösungen (engineered A. diadematus fibroin 3) angewendet wurde. Im Rahmen der Arbeit wurde eine Elektrospinnanlage …
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Strategies to design and optimize natural polymers as biomaterial inks for musculoskeletal tissue engineering applications
… Most of the research was carried out on silk fibroin, a protein commonly used for tissue engineering scaffold fabrication owing to its promising physical and biological properties, although it lacks the rheological properties required for extrusion-based bioprinting. Different approaches have …
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Synthesis and microfabrication of elastomeric biomaterials for advanced tissue engineering scaffolds
… microfluidic scaffolds using silk fibroin from the Bombyx mori as a bulk material. The need to combine the advantages of both natural proteins and synthetic polyesters motivated the synthesis and characterization of a new class of biodegradable elastomers termed …
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Behavior of a silkworm silk fiber web structure under wind load
… the crystalline region of the semi-crystalline fibroin and the extensibility from the length hidden within the amorphous region. The silk fiber is coated with sericin which acts as a glue connecting fibers together and as a matrix in the three-dimensional nonwoven multi-layer composite structure …
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