Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 12 of 12 for “"Fed-batch fermentation"”.
-
Optimal control of fed-batch fermentation processes
Optimisation of a fed-batch fermentation process typically uses the calculus of variations or Pontryagin's maximum principle to determine an optimal feed rate profile. This often results in a singular control problem and an open loop control structure. The singular feed rate is the optimal feed …
-
The application of fuzzy control to fed-batch fermentation
Fermentation processes are highly nonlinear and subject to variability. The fermentation's states are not readily available on-line and therefore the application of closed loop control schemes have been hindered. It was decided to investigate fuzzy control as it is able to deal with systems whose …
-
Comprehensive analysis of metabolic pathways through the combined use of multiple isotopic tracers
… the analysis of metabolic fluxes in E. coli in a fed-batch fermentation for overproduction of 1,3-propanediol (PDO).
-
Retention of fermentation biomass for extended L-Lysine fermentations
… it was demonstrated that the current L-lysine fermentation technology can be enhanced by continuously withdrawing spent medium while recycling the biomass in the culture suspension to the bioreactor. The biomass in the reactor outlet stream is separated from the spent medium using cross-flow …
-
Extremum-Seeking Control of Industrial-Scale Fermentation Processes
… control strategies in industrial fed-batch fermentation processes. The first paper describes a new control strategy for avoiding overflow metabolism in the exponential growth phase of a fermentation, based on analysis of the frequency spectrum of the dissolved oxygen measurement …
-
The Production of the Enzyme Dextransucrase by Batch and Continuous Fermentation Techniques
… concerning general enzyme biotechnology and fermentation technology. Fed-batch fermentation of the bacteria Leuconostoc mesenteroides NRRL B512 (F) to produce dextransucrase has formed the major part of this research. Aerobic and anaerobic fermentations have been studied using a 16 litre New …
-
Entwicklung und Anwendung einer Fed-batch-Betriebsweise mit Nährstofffreisetzungssystemen zur kontrollierten Kultivierung und zum Screening von Mikroorganismen in Schüttelreaktoren
… industrial production processes are performed in fed-batch operational mode. In contrast, the screenings for microbial production strains are run in batch mode which results in completely different physiological conditions than relevant for production. This may lead to wrong strain selections. …
-
BIODIESEL PRODUCTION THROUGH MICROWAVE ASSISTED TRANSESTERIFICATION OF MICROBIAL CELLS
… was studied. When cultured in a one-stage fed-batch process wherein crude glycerol and nitrogen source were fed intermittently for 12 days, the final biomass density and lipid content were 31.2 g/L and 44.2%, respectively. When cultured in a two-stage fed-batch operation wherein crude …
-
Improving the recovery of "difficult to release" periplasmically-expressed products from recombinant E. Coli
… of anti-lysozyme Fab D1.3 were determined during fed-batch cultivation of E. coli. It was shown that the Fab produced as both soluble and insoluble forms and released into the culture medium over the course of fed-batch fermentation. Purification of Fab D1.3 was then performed using various …
-
Feasibility for value addition to sucrose in South Africa through conversion to platform chemicals
… using Saccharomyces cerevisiae in a dual phase fed batch fermentation process. The overall design for the succinic acid process was based on the design proposed by Efe, et al (2013). A cost evaluation was then done on the design for an economic analysis. The economic analysis was done on the …
-
Novel Polyhydroxyalkanoate blends: their characterisation and possible applications
… flask cultures. The study was continued with batch fermentation studies in 2 litre fermenters and an enhanced PHA yield was observed with an optimum yield of 44.6% dcw. Further, an enrichment media (MEM media) for B. cereus SPV was modified, with three simultaneous nutrient limitations for the …
-
Optimização de estratégias de alimentação para identificação de parâmetros de um modelo de E. coli. utilização do modelo em monitorização e controlo
Os principais objectivos desta tese são: o desenho óptimo de experiências para a identificação de coeficientes de rendimento de um modelo não estruturado de um processo de fermentação semicontínua de Escherichia coli; a verificação experimental das trajectórias de alimentação obtidas por simulação; …