Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"FUCCI"”.
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HPV E6 inhibits E6AP to regulate epithelial homeostasis by modulating keratinocyte differentiation commitment and YAP1 activation
… infected basal cell to ensure persistence. Using FUCCI and cell-cell competition assays, we established the role of two common viral targets of low-risk and high-risk E6 proteins, E6AP and NHERF1, on the key components of epithelial homeostasis: cell density, proliferation, commitment to …
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Measuring ongoing chromosomal instability in single-cell DNA sequencing data
… using experimental cell multiplets, a FUCCI cell cycle expression system, and a benchmark against state-of-the-art methods. scAbsolute provides a robust foundation for single-cell DNA sequencing analysis across different technologies and for various downstream analyses. Second, we …
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TRANSCRIPTIONAL NETWORKS VARIATIONS DURING CELL CYCLE PROGRESSION IN HUMAN EMBRYONIC STEM CELLS
… to be fully uncovered. Here, I have utilised the FUCCI reporter system combined with ATAC-Seq to analyse chromatin dynamics during cell cycle progression in hESCs. Furthermore, I performed ChIP-Seq analyses to define the genomic location of transcriptional regulators during cell cycle progression …
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Understanding and Targeting Clearance of Persistent HPV Infections
… expression of a real-time cell cycle indicator (FUCCI) and early differentiation marker (KRT10); or the competition between differently fluorescently labelled infected versus uninfected cells. Here, it is presented that HPV16E6 expressing keratinocytes exhibited increased proliferation, delayed …
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Replicative Senescence is ATM-Driven, Reversible, and Delayed by Reduced ATM Activity at Low Oxygen
… instead entering telomeric crisis. Using FUCCI live-cell imaging, we establish that ATM inhibition allows a subset of wildtype senescent cells to re- enter the cell cycle and progress through several cell divisions. RNA-seq and p38 inhibitor culturing show ATM-inhibited post-senescent …
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Time to quit? Non-genetic heterogeneity in cell fate propensity after DNA damage
… Ubiquitination-based Cell Cycle Indicator (FUCCI) probes (Sakaue-Sawano et al., 2008), with semi-automated image analysis to characterise the response of single cells and their descendants to DNA lesions across multiple cell cycle generations. This approach, complemented by generational …
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Spatiotemporal dynamics of cell division in intestinal homeostasis
… ubiquitination-based cell cycle indicators (FUCCI). These ribbons were occasionally interrupted by cells in other cell cycle phases suggesting the separation of sisters by daughters from another lineage. This suggests that cell-cycle coordination can facilitate post-mitotic separation, and …
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Microfluidics and live imaging advances: applications in host/pathogen, immunity and stem cell single cell phenotyping
… CD4+ T cells. In Chapter 5, we incorporated the FUCCI cell reporter system in our single cell live imaging system to reveal the effect of different media conditions on the cell cycle progression and cell fate choices of mouse embryonic stem (mES) cells. By improving different factors such as …
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A Reductionist Approach to Modeling Human Corticogenesis
… embryo, we utilized transgenic lines expressing FUCCI (fluorescently ubiquitination cell cycle indicators). Together with single‐cell analysis, we were able to demonstrate self‐organization in radial progenitors, interkinetic nuclear migration, as well as requirement for Notch signaling for …