Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 6 of 6 for “"FTOC"”.
-
Nicotine and TNF alpha, modulators of T cell signaling-effects on T cell development in fetal thymus organ culture
… thymic stroma. Using fetal thymus organ culture (FTOC) as a model of T cell development, we investigated the ability of two potent signal modulators to influence this process. These studies show that both nicotine and tumor necrosis factor-alpha have the ability to influence T cell receptor (TCR) …
-
The Major Histocompatibility Complex (MHC) and the proteasome-ubiquitin pathway in T cell development
… We utilize a fetal thymic organ culture (FTOC) system with a panel of proteasome inhibitors to implicate the proteasome in thymocyte apoptosis and negative selection. We find that proteasome inhibitors do not completely block but rather delay both dexamethasone- and antigen-triggered …
-
Wnt Secretion Proteins Modulate RANKL-Induced Expression of Aire in Thymic Epithelial Cells
… TECs, we generate fetal thymic organ cultures (FTOC) in which we eliminate the developing thymocytes and instead use exogenous RANKL to drive TEC specific maturation, while simultaneously culture the TECs with different Wnt proteins. Our results show that the Wnt antagonist, Sfrp-1, and the Wnt …
-
Human-Mouse Neural Crest Chimeras as a Novel Model for Human Melanocyte Biology in Development and Disease
… feasible, we develop fetal thymic organ culture (FTOC) co-cultures with human NCCs. We find that human NCCs and NCC-mesenchyme have a unique capacity to efficiently engraft onto mouse thymus ex vivo and preliminary evidence suggesting partial central tolerance is possible. The success of these …
-
Phenotypic and functional analysis of the mesenchymal stromal cell compartment in the thymus
… thymus. In functional experiments involving FTOC, we show that mesenchymal cell-derived RA plays an important role in regulating cTEC numbers and provide initial evidence for a direct role of RA responses in TEC in regulating cTEC homeostasis in vivo. In addition to its direct role in …
-
Aspects of T cell development contributing to autoimmunity.
Autoimmunity, such as type 1 diabetes (T1D), arises as a result of tolerance breakdown against host tissues, i.e. the β-cells. Central tolerance is established during thymic T cell development and defects in key events during this process could contribute to the pathogenesis of autoimmune diseases. …