Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"FRET sensor"”.
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Applications of Engineered Proteins in Redox Biology and Biomarker Detection Assay Development
… They have been made into genetically encoded biosensors/probes or affinity agents for biomarker detection. This thesis explored a few topics using assays developed with engineered proteins. The genetically encoded hydrogen peroxide generator, D-amino acid oxidase (DAAO), was used to understand …
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USING CONJUGATED POLYMERS AS BIOLOGICAL SENSOR BASED ON FLUORESCENCE RESONANCE ENERGY TRANSFER
… this dissertation are to design novel molecular sensors based on fluorescence resonance energy transfer (FRET) between fluorophore (donor) and polydiacetylene (PDA, acceptor) for selective detection of biomolecules in solution. The work described in this dissertation is divided into three …
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Smart, biocompatible semi-conductor nanocrystal constructs designed for in-vitro imaging applications
… by incorporating the nanocrystals into a dynamic sensor construct and taking measurements in a bioenvironment. A dye construct that can act as a Fluorescent Resonant Energy Transfer (FRET) acceptor is conjugated to the FRET donor QD through a molecular linker whose conformation changes depending …
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Engineering protein self-assembly for biosensing applications
… we engineered protein-based fluorescent biosensors for nitrite detection, employing a Förster Resonance Energy Transfer (FRET) system. A nitrite-binding protein complex, NasS-NasT, was coupled with a fluorescent protein FRET pair, and the dissociation of this complex in response to nitrite …
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Oncogene-induced remodelling of cellular networks
… an isogenic panel of cells stably expressing a FRET sensor and developed an experimental pipeline including automated biochemical imaging, microfluidic-based stimulation, and custom image analysis. Using these tools, I have identified differ- ential signalling dynamics and heterogeneity in …
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Oncogenic KRAS drives heterogeneity in metabolic and signalling pathways through genetic and non-genetic mechanisms
… mutations, focusing mainly on G12D. We used a FRET sensor reporting mitochondrial ATP levels in live cells to study G12D cells at multiple time points. We found an early drop in mitochondrial ATP which was only identified when considering intracellular heterogeneity. This drop was followed by a …