Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"FRET reporter"”.
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Developing a co-culture model of ovarian cancer to identify immunotherapies that can overcome TGF-β-induced immunosuppression
… Förster resonance energy transfer (FRET) reporter and either the chicken ovalbumin (OVA) epitope or scrambled (SCR) epitope control. Co-culturing OVA cells with OVA-specific OT-1 T cells resulted in decreased FRET-positive cells, indicating OT-1 T cells effectively target OVA cells. …
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Measuring Single Cell Responses to Lapatinib In A Heterogeneous Population
… constrained and tested using live cell FRET reporter constructs.</p>
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Quantitative Analysis of the Structural Dynamics of Mitotic Chromosomes in Live Mammalian Cells
… intensities; and molecular scale (~10 nm) by a FRET reporter of histone tail environment. These measurements show that (i) mitotic compaction may start at least 20 min before prometaphase; (ii) it correlates with changes in histone tail conformation; (iii) chromatin density is not highest in …
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Novel Fluorescent Sensors for Dynamic Intracellular Signalling
… This thesis presents a novel fluorescent reporter for PKC activity in living cells. The kinase C probe (KCP-1) consists of a PKC specific substrate sequence that is flanked by two fluorescent proteins, which are capable of fluorescence resonance energy transfer (FRET). The substrate …
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New roles of Rac-GEFs in Neutrophils and in Glucose Homeostasis
… the activation of GPCRs. I used our Rac activity FRET reporter mouse strain (RFC) (Johnsson, Dai et al. 2014), crossed to mice deficient in the Rac-GEFs P-Rex1/Vav1, DOCK2 or Tiam1. I demonstrated that Rac activity is increased in RFC Tiam1–/– neutrophils adhering to integrin ligands, which may …
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The intramolecular domain interactions and phosphatase activation mechanisms of Shp2
… and functions of Shp2. Here, we developed a FRET-based Shp2 reporter, a single molecule comprised of the full length human Shp2 concatenated in between a FRET pair, ECFP and YPet. In vitro incubation of Shp2 reporter with PDGFRβ induced a significant FRET increase via intramolecular …