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Showing 1 to 20 of 22 for “"FPLC"”.

  1. Protein Preservation in Fossil Whale Bones of the Miocene/Pliocene Pisco Formation, Peru

    Vidal, Uriel Leme

    … and Fast Performance Liquid Chromatography (FPLC) for proteins/peptides size separation. The FPLC fractions were analyzed by Matrix-assisted Laser Desorption/ Ionization-Time of Flight (MALDI-TOF) for a more specific identification of the proteins/peptides sizes. Polyamide sheet Thin-Layer …

    loma-linda Repository record

  2. Aprimoramento do diagnóstico de Leishmaniose visceral canina por ELISA usando o antígeno NTPDase-2 de Leishmania (Leishmania) infantum chagasi e padronização do diagnóstico por imunocromatografia

    Souza, Anna Cláudia Alves deUniversidade Federal de Viçosa2016

    … foi feita a purificação manual e automatizada (FPLC). As purificações manuais forneceram maior grau de pureza e o processo em FPLC forneceu maior rendimento. Assim, várias purificações foram testadas, a fim de obter alto grau de pureza em sistema automatizado. Novos ELISAs foram realizados …

    brazil-ufv Repository record

  3. Arginase from Bacillus caldovelox

    Patchett, Mark L.The University of Waikato1988

    … ion exchange chromatography, Superose 12 GP-FPLC, Mono Q IE-FPLC and phenyl-Superose HI-FPLC. B. caldovelox arginase has a Mᵣ of ~176000 and a subunit Mᵣ of 32000, suggesting a hexameric subunit structure. It has an isoelectric point of 5.4, a pH optimum for activity of 9 (at 60°C in 0.1M …

    waikato-masters Repository record

  4. Venom Yield, Regeneration, and Composition in the Centipede Scolopendra Polymorpha

    Cooper, Allen Michael

    … that venom protein components, separated by RP-FPLC, undergo asynchronous synthesis. During the first 48 hours, volume and protein mass increased linearly. However, protein regeneration lagged behind volume regeneration, with only 65–86% of venom volume and 29–47% of protein mass regenerated …

    loma-linda Repository record

  5. tRNA synthetases as potential RNA capping enzymes

    Říha, JanUniverzita Karlova, Přírodovědecká fakulta2024

    … (fast protein liquid chromatography - FPLC). Následně byly tRNA syntetázy testovány pro syntézu volných dinukleosid polyfosfátů, díky čemuž jsme zjistili optimální reakční podmínky. Za daných reakčních podmínek jsme následně testovali, zda jsou tRNA syntetázy schopny vytvořit NpnN-RNA, …

    charles-prague Repository record

  6. Untersuchungen zur Physiologie des Essigsäurebakteriums Gluconobacter oxydans 621H

    Hoffmeister, Marc2012

    … korrespondierende Dehydrogenase wurde mittels FPLC gereinigt und per nano-ESI-MS/MS als membrangebundene Alkohol-Dehydrogenase identifiziert. Die Ergebnisse dieser Arbeit geben einen Einblick in die genomweite Transkriptionsregulation von G. oxydans 621H und bieten somit eine Grundlage zur …

    goettingen Repository record

  7. Purification and characterization of a protein phosphatase (PP1-Arch) from the archaebacterium Sulfolobus solfataricus, isolation and expression of its gene

    Leng, JieVirginia Tech1994

    … gel filtration (G- 100), and Mono Q FPLC chromatography. PP1-Arch was identified from the final purified sample by renaturation on an SDS-polyacrylamide gel. The molecular size of PP1-Arch was determined by both gel filtration chromatography and SDS-PAGE as 28 kDa and 33 kDa, …

    vt Repository record

  8. 2D layout of bead based thioaptamer/aptamer selection platform for therapeutics and diagnostics

    Xu ZhaoThe University of Texas Medical Branch2007

    … and verified. Thioaptamer purification using FPLC and HPLC was also investigated, and several 5’-funtionalized thioaptamers were successfully purified. Electrophoretic mobility shifting assay (EMSA) has been used to verify XBY6 binding, and ELISA assay has been used to verify I-kappa B binding …

    utmb Repository record

  9. Studies on the carbon monoxide dehydrogenase enzyme complex present in acetate-grown Methanosarcina thermophila strain TM-1

    Terlesky, Katherine C.Virginia Polytechnic Institute and State University1989

    … fractionated with ammonium sulfate treatment and FPLC phenyl superose chromatography. Two peaks of methyltransferase activity were observed in each cell extract sample following phenyl superose fractionation.

    vt Repository record

  10. Production and characterisation of novel recombinant antibody fragments against sialic acid

    Donohoe, Gerard GaryDublin City University2011

    … properties wereassessed by ELISA, HPLC, FPLC, and SPR. One clone, AE8, displayed the strongest reactivity to a panel of different sialylated structures. In addition, SPR inhibition andkinetic analysis revealed that the AE8 scFv had nanomolar affinity for the Neu5Gc-BSA neoglycoconjugate. …

    dcu Repository record

  11. A study of the aggregating cationic antibacterial proteins and peptides in bovine seminal plasma

    Hameed, ImranThe University of Waikato1987

    … disaggregation of the antibacterial aggregates. FPLC of diluted samples using acetonitrile and trifluoroacetic acid was found to give complete disaggregation. The aggregates were found to be made up of two peptides, a basic antibacterial peptide with a molecular weight of about 1.2 kDa and an …

    waikato-masters Repository record

  12. Anti-<i>Candida a</i>ctivity of killer toxins from the yeast <i>Williopsis mrakii</i>

    Hodgson, Valerie1993

    … Da. Rapid purification of the toxin using FPLC techniques was hampered by problems in accurately assessing toxin activity in the presence of high salt concentrations. This was overcome by the development of a novel colourimetric assay of toxin activity.

    abertay Repository record

  13. Purification and characterization of a selective growth regulator for human myelopoietic progenitor cells

    Shellard, Joan E.University of British Columbia1993

    … of leukemic cellular lysates, followed by FPLC molecular size fractionation and/or preparative SDS-PAGE. The myelopoietic activity was located within a30-35 kDa molecular weight fraction (P30), and the P30 fraction was consistently found to be selective in its inhibition of normal myeloid …

    ubc Repository record

  14. Gold Nanoparticles as a Delivery System of Oligonucleotides into the Brain

    Gromnicova, RadkaThe Open University2017

    … amounts of bound DNA were fractionated by FPLC and analysed by a novel electrophoretic mobility shift assay (EMSA). When comparing the uptake efficiency of dsDNA/galactose nanoparticles to galactose nanoparticles there was no reduction in uptake efficiency, despite addition of the highly …

    the-open-u Repository record

  15. Validation of shellfish isolates (glycosaminoglycans, GAGs) for development as a novel anti-tumour therapy for children : GAG action on lymphocytes/T-regulatory cells

    Jones, CF2026

    … and naïve. Phase 3 studies evaluated individual FPLC separated GAG fractions to establish potentially potent domains of the compounds. This work also focussed on T-regulatory cell (Treg) responses to the GAGs to provide an insight to their potential modulation of a population of cells associated …

    salford Repository record

  16. Functional and developmental characterisation of matrix binding sites in decapentaplegic

    Roth, MartinUniversität Würzburg

    … Different matrix binding was shown by Heparin FPLC. The biological relevance of the matrix interaction of DPP was examined in transgenic flies. To allow expression of the different variants under the control of various Gal4 driver lines, they were cloned behind an UAS-promoter site. In early …

    wurz-thes Repository record

  17. An Analysis of the Role of Bacillus oleronius Proteins in the Pathogenesis of Ocular Rosacea

    McMahon, Frederick W.National University of Ireland Maynooth2015

    … by anion exchange chromatography and ÄKTA FPLC™ for their use in further assays. The expression of the immuno-reactive 62 kDa GroEL protein was analysed in different culture conditions to determine a possible role in the pathogenesis of the disease, and it was observed that the B. oleronius …

    maynooth Repository record

  18. The immobilisation of Thermus strain Rt41A proteinase and its use in peptide synthesis

    Wilson, Shelley-AnnThe University of Waikato1991

    … to apparent homogeneity using ion exchange and FPLC chromatography and had a final specific activity of approximately 9000 azocasein units/mg. The substrate specificity of the proteinase against various peptide p-nitroanilide substrates was investigated and compared with the results of Peek …

    waikato-masters Repository record

  19. Intracellular growth of Brucella abortus and B. melitensis in murine macrophage-like cell lines and partial characterization of a biologically active extract from B. abortus strain RB51

    Wise, Darla J.Virginia Tech1995

    … or by fast performance liquid chromatography (FPLC). Therefore, SDS-polyacrylamide gel electrophoresis (SDS-PAGE) was used to separate S2 into individual proteins. Two proteins, 10.2 kDa and 11.5 kDa in size, were observed to stimulate the production of INF-γ by sensitized splenocytes. The …

    vt Repository record

  20. Constituintes químicos da madeira-de-lei Myracrodruon urundeuva com propriedades antioxidantes e ação contra Fungos, Bactérias e Insetos

    SÁ, Roberto AraújoUniversidade Federal de Pernambuco2008

    … em coluna Hiprep 16/60 Sephacryl S-300/Äkta-FPLC. MuHL foi analisada em eletroforese em gel de poliacrilamida para proteínas nativas básicas ou ácidas e em condições desnaturantes e redutoras (SDS-PAGE). MuHL também foi avaliada quanto à natureza glicoprotéica. A AH de MuHL em presença de …

    brazil-ufpe Repository record

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