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Showing 1 to 2 of 2 for “"FLP/FRT recombination"”.
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Nucleolar Stress Due to Depletion of Nopp140 in Drosophila melanogaster
… we deleted the Nopp140 gene in Drosophila using FLP-FRT recombination. Genomic PCR, RT-PCR, and immunofluorescence microscopy confirmed the loss of Nopp140, its mRNA, and protein products. Nopp140-/- larvae arrested in the second instar stage and died within 8 days. While nucleoli appeared intact …
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FLP-mediated conditional loss of an essential gene to facilitate complementation assays
… is placed between target sequences recognized by FLP recombinase from the yeast 2 μm plasmid. This construct is transformed into a plant heterozygous for a null mutation at the endogenous locus, and progeny plants carrying the excisable complementing gene and segregating homozygous knockout at the …