Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"FKBP"”.
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Biochemical and Structural Studies of the Role of FKBP(L)s in the fly and Mammalian Circadian Cycle
FK506 binding-like proteins (FKBPLs) are proteins that structurally resemble the immunophilins. Their functions in cell signaling include protein folding, molecular trafficking, RNA splicing and cell cycle regulation among others. Bride of Doubletime (BDBT) previously designated as CG17282 in the …
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Kombination von Substratanreicherung und Katalyse als generelles Prinzip von Faltungshelferenzymen
… die Peptidylprolylisomerase SlpA, die aus einer FKBP-Domäne und einer Chaperondomäne aufgebaut ist. Die FKBP-Domäne besitzt Prolylisomeraseaktivität, die IF-Domäne ist für die Chaperoneigenschaften von SlpA verantwortlich. SlpA besitzt eine sehr geringe Prolylisomerase- und …
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Regulation of Skeletal Muscle Differentiation by a Rapamycin -Sensitive Signaling Pathway
… the protein is completely dispensable. Both the FKBP 12-rapamycin binding domain (FRB) and the kinase domain are necessary for mTOR's myogenic function despite kinase activity being dispensable. These observations provide structural insights into the regulatory mechanisms of mTOR, possibly …
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Untersuchung einer Calcineurin-bindungsdefizienten FKBP12.6-Mutante in Kardiomyozyten
… Aktivität gesteuert. Durch die Bindung von FKBP12.6 an den RyR2, die Isoform der Herzmuskelzelle, wird die Offenwahrscheinlichkeit des Kanals und damit der Calciumverlust in der Diastole herabgesetzt. Die durch FKBP12.6 koordiniertere Öffnung benachbarter RyR (coupled gating) bedingt zudem …
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FKBP14 in Notch Signalling and Drosophila Development
… are unclear. A screen in Drosophila identified FKBP14, an FK506-binding proteins (FKBP), as a genetic modifier of Presenilin (Psn), with FKBP14 mutants exhibiting defects typical of loss of Notch signalling. FKBPs are highly conserved and involved in a wide array of biochemical processes …
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Human cohesin dynamics and their regulation in M and S phase
… (telophase) gates by employing the so-called FRB/FKBP system, allowing me to artificially close each of cohesin's gates individually in a conditional manner. I found that cohesin's DNA exit gate during prophase is composed of Smc3 and Scc1. To load the complex onto DNA, however, the gate situated …
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Investigation towards the role of LmxOSM3.1 phosphorylation and the identification of its interaction partners using an improved method of proximity labelling
… (mTID) and LmxOSM3.1 (PoI) are fused to FKBP and FRB (of the FRB-FKBPmammalian oligomerisation system), respectively. A cell line expressing the CRISPR-Cas9 machinery and mTID fused to FKPB was generated but not yet assessed on a molecular level. Likewise, LmxOSM3.1 and LmxMPK13 tagged …
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Adenovirus-mediated gene transfer of FK506-binding proteins FKBP12.6 and FKBP12 in failing and non-failing rabbit ventricular myocytes
Die FK-506 Bindungsproteine (FKBP) können in Skelettmuskel (FKBP12) und Herzmuskel (FKBP12.6) die Kalziumfreisetzung aus dem sarkoplasmatischen Retikulum (SR) durch Bindung am Ryanodin Rezeptor (RyR) beeinflussen. FKBP12.6 bindet an den kardialen RyR (RyR2) und FKBP12 ausschließlich an den …
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THE ROLE OF ATP AND FK-506 BINDING PROTEIN IN THE COUPLED GATING OF SKELETAL RYANODINE RECEPTORS
… and attributed to FK-506 binding protein 12 (FKBP12) associating with neighboring RyR1s the stabilization of RyR1-RyR1 interactions that promote coupled gating. Previous studies suggested that ATP is required for coupled RyR1 gating; however, the mechanism by which ATP promotes the coordinated …
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Advancements in genome engineering and applications in mammalian systems
… with chemically inducible dimerizers GAI-GID, FKBP-FRB and ABI-PYL1 respectively. The dCas9 protein was fused with one of the dimerizers and the other dimerizer was fused with activator domain p65-HSF1. Addition of inducers enabled dimerization which resulted in the recruitment of p65-HSF1 …
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Investigating the cytoskeleton and microtubule-based transport with in situ cryo-electron tomography
… clustering system that harnessed the inducible FKBP-FRB binding system. Cryo-CLEM allowed the targeting and identification of possible clusters in lamellae; however, tomograms lacked microtubules, which is an essential pre-requisite for visualising motile dynein-dynactin complexes. I next …
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Das Gen-3-Protein filamentöser Phagen als Modellsystem zur Untersuchung von Proteinstabilität, Faltungsmechanismen und Prolylisomerisierung
… die Substratspezifität von Prolylisomerasen der FKBP-Familie untersuchen zu können, wurde eine Bibliothek von N2-Varianten mit allen natürlichen Aminosäuren vor Pro161 hergestellt. Prolylisomerasen, die nur aus einer katalytischen Domäne bestehen, zeigen für kurze Peptide und für faltende …
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Charakterisierung modularer Peptidyl-Prolyl-cis-trans-Isomerasen
… zeigen, dass SlyD, FkpA und SurA aus E. coli, FKBP18 aus Thermococcus sp. KS-1 und TF aus Thermotoga maritima gegenüber faltenden Proteinsubtraten eine deutlich höhere Prolylisomeraseaktivität zeigen als eine isolierte FKBP-Domäne. Nur FKBP22 aus Neurospora crassa besitzt eine sehr schwache …