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Showing 1 to 20 of 122 for “"FITC"”.

  1. Synthesis and characterisation of fluorescent FITC-insulin glulisine conjugates for insulin delivery

    … glulisine (Apidra®) and the fluorescent dye FITC (Fluorescein Isothiocyanate) for comparison with the biological activity of native insulin glulisine. The first objective of the study was to produce a single-labelled fluorescent-insulin conjugate (FITC-insulin glulisine), achieved without …

    de-montfort Repository record for Synthesis and characterisation of fluorescent FITC-insulin glulisine conjugates for insulin delivery (opens in a new tab)

  2. Synthesis, characterisation and biological activity of FITC-insulin for the development of an artificial pancreas

    … was to produce a fluorescein isothiocyanate (FITC)-insulin conjugatewhich shows equivalent biological activity to native insulin under novel reaction conditions without theneed for using protecting groups and multi-step synthetic conditions. Secondly, the stability andsolubility profiles of …

    de-montfort Repository record for Synthesis, characterisation and biological activity of FITC-insulin for the development of an artificial pancreas (opens in a new tab)

  3. Intestinal Inflammation Model Inducing Gut Leakage by Different Methods in Broiler Chickens Using FITC-d as a Marker

    … of fluorescein isothiocyanate dextran (FITC-d) and its passage into blood can be used as a marker for tight junction permeability. FITC-d is a large molecule (3-5 kDa) which does not usually leak through the intact gastrointestinal tract barrier. However, when conditions disrupt the …

    arkansas Repository record for Intestinal Inflammation Model Inducing Gut Leakage by Different Methods in Broiler Chickens Using FITC-d as a Marker (opens in a new tab)

  4. Understanding the barriers to, and impact of, men's engagement in physical activity and health related behaviours : an examination of an English Premier League football in the community men's health programme

    … Football Club's Football in the Community (FitC) scheme; Everton in the Community (FitC) and Liverpool John Moores University, School of Sport and Exercise Sciences. In recent years, there has been recognition of the influence that English Premier league (EPL) football clubs can have in …

    liverpool-jm Repository record for Understanding the barriers to, and impact of, men's engagement in physical activity and health related behaviours : an examination of an English Premier League football in the community men's health programme (opens in a new tab)

  5. Investigation into reliability and performance of an implantable closed-loop insulin delivery device

    … device, fluorescently labelled human insulin (FITC-insulin) was synthesised and characterised using RP-HPLC and MS, to produce a product with predominantly di-labelled conjugate (>75%) with no unreacted FITC or native insulin. Clinically used insulin analogues were also fluorescently labelled …

    de-montfort Repository record for Investigation into reliability and performance of an implantable closed-loop insulin delivery device (opens in a new tab)

  6. PHYSICO-CHEMICAL STUDIES OF THE VESICULAR GLUTAMATE TRANSPORTER 1 (VGLUT1)

    … our understanding of the membrane topology, FITC was used to modify VGLUT1 in solution. On average, 5.35 ¡À 1.10 lysines were labeled with FITC in each VGLUT1 molecule. Trypsin, endoproteinase Glu-C and Arg-C were used to digest FITC labeled VGLUT1 for mass spectrometry analysis. Mass …

    montana-tech Repository record for PHYSICO-CHEMICAL STUDIES OF THE VESICULAR GLUTAMATE TRANSPORTER 1 (VGLUT1) (opens in a new tab)

  7. PHYSICO-CHEMICAL STUDIES OF THE VESICULAR GLUTAMATE TRANSPORTER 1 (VGLUT1)

    … our understanding of the membrane topology, FITC was used to modify VGLUT1 in solution. On average, 5.35 ¡À 1.10 lysines were labeled with FITC in each VGLUT1 molecule. Trypsin, endoproteinase Glu-C and Arg-C were used to digest FITC labeled VGLUT1 for mass spectrometry analysis. Mass …

    montana Repository record for PHYSICO-CHEMICAL STUDIES OF THE VESICULAR GLUTAMATE TRANSPORTER 1 (VGLUT1) (opens in a new tab)

  8. The photophysical characterisation of a fluorescence-based immunoassay for the detection of gonadotropin-releasing hormone, type-1 (GnRH-I)

    … 9-amino acid peptide, [des-pGlu¹]-LH-RH-Acp-FITC, is introduced to compete with GnRH-I for the two binding sites on the antibody 7B10.1D10. The core results demonstrate a photophysical characterisation of the binding of [des-pGlu¹]-LH-RH-Acp-FITC and 7B10.1D10 in homogeneous solution based on …

    strathclyde Repository record for The photophysical characterisation of a fluorescence-based immunoassay for the detection of gonadotropin-releasing hormone, type-1 (GnRH-I) (opens in a new tab)

  9. The Potential of Cellulose Nanocrystals in the Detection and Treatment of Cancer

    … imaging agent fluorescein-5\'-isothiocyanate (FITC) to create FITC-CNC-FA; CNCs have also been conjugated with doxorubicin (DOX), a potent chemotherapeutic (DOX-ALAL-CNC-FA). We have determined FITC-CNC-FA's and DOX-ALAL-CNC-FA's ability to specifically target FR-positive cancer cells in vitro; …

    vt Repository record for The Potential of Cellulose Nanocrystals in the Detection and Treatment of Cancer (opens in a new tab)

  10. Dendrimer Modified Silica Nanoparticles as Fluorescent Chemosensors for the Detection of Copper and Cyanide Ions

    … (RITC) or fluorescein isothiocyanate (FITC) fluorescent dyes. In the presence of Cu<sup>2+ </sup>ions, the emission of the fluorescent dye is quenched by Forster resonance energy transfer (FRET) and is dependent upon dendrimer generation, pH, fluorescent dye, and surface charge. The …

    usfca Repository record for Dendrimer Modified Silica Nanoparticles as Fluorescent Chemosensors for the Detection of Copper and Cyanide Ions (opens in a new tab)

  11. Einfluss der Osteopontin Defizienz bei Mäusen auf allergische Hautreaktionen vom Spättyp

    … nach Applikation des obligaten Kontaktallergens FITC eine verminderte Migration von LZ aus der Epidermis in die regionalen, Haut-drainierenden Lymphknoten. Auf T-Zell-Ebene fand sich in den Lymphknoten der OPN-defizienten Tiere 48 Stunden nach FITC-Applikation eine geringere Anzahl CD4+ und CD8+ …

    freiburg-diss Repository record for Einfluss der Osteopontin Defizienz bei Mäusen auf allergische Hautreaktionen vom Spättyp (opens in a new tab)

  12. Development of a phosphotriesterase enzyme assay for determination of organophosphate pesticides

    … was labeled with fluorescein isothiocyanate (FITC), was immobilized on polymethylmethacrylate beads and the pH change is monitored through the pH-sensitive quantum yield of FITC. Analytes were measured using a KinExA fluorescence analyzer. This assay can detect paraoxon concentration down to …

    unlv Repository record for Development of a phosphotriesterase enzyme assay for determination of organophosphate pesticides (opens in a new tab)

  13. ACTION RESEARCH: UNDERSTANDING THE EFFECTIVENESS OF AN ENGLISH PREMIER LEAGUE FOOTBALL IN THE COMMUNITY HEALTH IMPROVEMENT INTERVENTION

    … Football Club’s Football in the Community (FitC) scheme; Everton in the Community (EitC) and Liverpool John Moores University. In recent years, there has been recognition of the influence that English Premier League football clubs can have in attending to the health improvement agenda …

    liverpool-jm Repository record for ACTION RESEARCH: UNDERSTANDING THE EFFECTIVENESS OF AN ENGLISH PREMIER LEAGUE FOOTBALL IN THE COMMUNITY HEALTH IMPROVEMENT INTERVENTION (opens in a new tab)

  14. TDI 천식 기도염증반응과 관련된 활성산소의 역할

    … polymerase chain reaction 15 3. Preparation of FITC-DBP conjugate and immunofluorescence staining 16 4.Flow cytometry analysis 16 5. Western blot analysis 17 6. Measurement of 1,25(OH)2D3 production and VEGF secretion 17 7. Statistical Analyses 18 III. RESULTS 19 A. Results 19 1. TDI-HSA …

    ajou Repository record for TDI 천식 기도염증반응과 관련된 활성산소의 역할 (opens in a new tab)

  15. CAPILLARY ELECTROPHORESIS ANALYSIS OF SINGLE-STRANDED DNA APTAMERS AND THEIR INTERACTIONS WITH PROTEIN TARGETS AND CARBON DOTS

    … determine the dissociation constant of a unique FITC-labeled single-stranded DNA and anti-FITC antibody system in free solution environment using the method of non-equilibrium capillary electrophoresis of non-equilibrium mixtures (NECEEM). The free-solution binding constant was 316 ± 17 nM. The …

    wfu Repository record for CAPILLARY ELECTROPHORESIS ANALYSIS OF SINGLE-STRANDED DNA APTAMERS AND THEIR INTERACTIONS WITH PROTEIN TARGETS AND CARBON DOTS (opens in a new tab)

  16. SYNTHESIS, SURFACE MODIFICATION, AND CHARACTERIZATION OF BIOCOMPATIBLE MULTIFUNCTIONAL NANOPARTICLES

    … method, while, the hydrophobie dye, FITC (fluorescein isothiocyanate), was loaded in MSNs by using a sol-gel method. The FITC covalently bonded with MSNs shows better performance in term of photostability. To increase the cell uptake, the surface of FITC loaded MSNs were further …

    uwo Repository record for SYNTHESIS, SURFACE MODIFICATION, AND CHARACTERIZATION OF BIOCOMPATIBLE MULTIFUNCTIONAL NANOPARTICLES (opens in a new tab)

  17. The characterisation of a recombinant cytosolic shuttle based on ricin toxin

    … An investigation into the trafficking of the FITC-conjugated PAAs, ISA1-FITC and ISA23-FITC, supported PAA mediated cytosolic delivery. In this experiment, ISA1-FITC and ISA23-FITC polymers were non-toxic up to a concentration of 3mg/mL in both Vero and B16 cells and the accumulation of …

    greenwich Repository record for The characterisation of a recombinant cytosolic shuttle based on ricin toxin (opens in a new tab)

  18. Design and development of a site specific protein patterning technique for use in a microfluidic antibody separation device

    … The conjugation of streptavidin, streptavidin-FITC, NeutrAvidin, single domain protein L and multidomain protein L to silicon surfaces, upon reaction with UANHS, were investigated. Fluorescently labelled probes that associated with the protein motifs were used to confirm successful conjugation …

    soton Repository record for Design and development of a site specific protein patterning technique for use in a microfluidic antibody separation device (opens in a new tab)

  19. Discovery of Natural Product-based Antimycobacterial Agents Effective against Non-replicating Bacilli

    … fluorescent probes, Park’s nucleotide-Nε-C6-FITC (32, for MraY/MurX) and UDP-glucosamine-C6-FITC (46, for WecA) were developed. These fluorescent probes enable us to screen the polyprenyl-phosphate N-acetylhexosamine 1-phosphate transferase enzyme superfamily (e.g. MraY/MurX, WecA, AlgH, and …

    tenn-hsc Repository record for Discovery of Natural Product-based Antimycobacterial Agents Effective against Non-replicating Bacilli (opens in a new tab)

  20. Alternative strategies to incorporate biomolecules within electrospun meshes for tissue enginering

    … isothiocyanate labelled bovine serum albumin (FITC-BSA) loaded chitosan-alginate (CS-AL) microspheres were fabricated. The effects of cation to alginate ratio, type of alginate and concentration of CaCl2 on microsphere size, FITC-BSA loading and release were systematically evaluated. The CS-AL …

    vt Repository record for Alternative strategies to incorporate biomolecules within electrospun meshes for tissue enginering (opens in a new tab)

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