NDLTD logo NDLTD Union Catalog and Global ETD Search

Global ETD Search

Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.

Results

Showing 1 to 20 of 59 for “"FFPE"”.

  1. Focused Ultrasound Extraction (FUSE) for Formalin-Fixed, Paraffin Embedded (FFPE) DNA Extraction

    Mehochko, Isabelle GraceVirginia Tech2023

    Formalin-fixed, paraffin embedded (FFPE) tissue is the most abundant, accessible, and versatile tissue sample type available for genetic research and clinical applications. However, FFPE DNA extraction presents unique challenges and requires lengthy incubation periods, which can be impractical for …

    vt Repository record

  2. Implementation and Cost Analysis of Performing Molecular Assays using FFPE Tissue Blocks in the Clinical Laboratory at VA Palo Alto Health Care System

    Comandante, Donna2017

    … to process Formalin-Fixed Paraffin-Embedded (FFPE) tissue blocks.</p> <p>At VA Palo Alto Health Care System, FFPE samples for BRAF, KRAS and EGFR mutation detection assays are usually sent out to reference laboratories. However, with the increasing demand and cost of these tests, it would be …

    dominican Repository record

  3. Development of methods for the microbiome analysis of formalin fixed paraffin embedded tissue specimens

    Flores, YensiUniversity College Cork2019

    … under study. Formalin-fixed, paraffin-embedded (FFPE) samples represent the most comprehensive collections of patient materials in hospital pathology archives. However, for this sample to become reliably accessible for microbiome studies, the effects of FFPE processing on bacteria must be …

    cork Repository record

  4. Evaluation of a Paratuberculosis Quantitative Polymerase Chain Reaction Assay with Microscopic Correlation

    Tyler, Ronald Dale Jr.Virginia Tech2012

    … 85 formalin-fixed paraffin-embedded (FFPE) tissues from ruminants diagnosed with Johne's disease were tested with a commercial paratuberculosis quantitative polymerase chain reaction (qPCR) assay and had a sensitivity of 92%. To determine assay specificity, 21 FFPE tissues from animals …

    vt Repository record

  5. Statistical Methods For Two Problems In Cancer Research: Analysis of Rna-Seq Data From Archival Samples and Characterization of Onset of Multiple Primary Cancers

    Li, Jialu, <p>orcid.org/0000-0001-9772-3886</p>2018

    … to formalin-fixation and paraffin-embedding (FFPE) tumor samples for reliable diagnostic development. We designed a biospecimen study to directly compare gene expression results from different protocols to prepare libraries for RNA-seq from human breast cancer tissues, with randomization to …

    uthsc Repository record

  6. HLA-A Typing in formalin-fixed paraffin embedded tissue samples : towards potential retrospective analysis

    Leon Rodriguez, Daniel ArturoUniversidad del Rosario2011

    … embebidas en parafina y fijadas con formalina (FFPE en inglés) son recolectadas rutinariamente en oncología. Este procedimiento podría ser utilizado como una buena fuente de ADN, dado que en estudios en el pasado los ensayos de recolección de ADN no eran normalmente llevados a cabo de casi …

    rosario Repository record

  7. Immune Checkpoint Molecule Expression in Canine Lymphoma and Canine Reactive Lymphoid Hyperplasia

    Clothier, Stacy LaurenVirginia Tech2019

    … Retrospective: formalin-fixed paraffin-embedded (FFPE) tissue from dogs with untreated lymphoma (n=10) and reactive lymphoid hyperplasia (n=10). Prospective: fine-needle aspirates (FNAs) from dogs with untreated lymphoma (n=10) and reactive lymphoid hyperplasia (n=10). Total RNA was extracted, and …

    vt Repository record

  8. Human Papillomavirus DNA extraction and genotype analysis by multiplex real time polymerase chain reaction from formalin fixed paraffin wax-embedded cervical carcinoma specimens

    Price, BrendonDepartment of Pathology2019

    … of high quality genomic DNA from archival FFPE cervical squamous cell carcinoma cases and identify hrHPV genotypes by multiplex real time PCR (RT-PCR). Materials and methods: A retrospective search identified a total of 57 cases of cervical squamous cell carcinoma for the period 2004-2014. …

    cape-town Repository record

  9. Characterization of the Expression and Pathogenicity of Alpha-Synuclein in the Enteric Nervous System

    Kintrup, CarmenChristian-Albrechts-Universität zu Kiel

    … subjects. Formalin-fixed and paraffin-embedded (FFPE) full-thickness colon resections were used to characterize α-Syn localization pattern in the ENS of control subjects. Additionally, α-Syn localization pattern was assessed by immunohistochemistry in FFPE deep rectal biopsies of PD patients …

    cau-kiel Repository record

  10. Characterisation of Copy Number Changes in the Progression of Barrett’s Oesophagus

    Gregson, EleanorUniversity of Cambridge2018

    … changes in formalin fixed paraffin embedded (FFPE) tissue (Scheinin et al., 2014). We hypothesised that copy number alterations may be valuable markers in disease progression and aimed to characterise them in the progression of Barrett’s using sWGS in order to predict progression in patients …

    cambridge Repository record

  11. Bluttest statt Mammografie-Sreening?

    Krüger, SandraChristian-Albrechts-Universität zu Kiel

    … nachweisbar sind. Analysiert wurden FFPE-Tumorgewebeproben von DCIS, B2/B3-Läsionen sowie frühen invasiven Mammakarzinomen verschiedener molekularer Subtypen. Der Schwerpunkt liegt auf vier in der Literatur beschriebenen CpG-Markern: cg21501525 (TSHZ3), cg20072171 (FEZF2), cg16304215 …

    cau-kiel Repository record

  12. Translational phosphoproteomics methods to identify biomarkers and novel therapeutic targets

    Kohale, Ishwar N.Massachusetts Institute of Technology2021

    … sections of formalin fixed paraffin embedded (FFPE) clinical tissue specimens, opening the doors for direct translational insights from FFPE tumor tissue banks in hospitals. In the second part, we present an integrative platform using mass spectrometry imaging, phosphoproteomics and multiplexed …

    mit Repository record

  13. Investigation of protein biomarkers in HIV positive and HIV negative associated DLBCL

    Hlatshwayo, LeratoDepartment of Clinical Laboratory Sciences2020

    … in detail, protein distribution profile from FFPE tissue in HIV and non-HIV related DLBCL subtypes. Methods: FFPE DLBCL lymph node tissue samples from HIV and non-HIV related DLBCL were subjected to MALDI-imaging, in order to get the spatial distribution of proteins in DLBCL tissue. Proteins …

    cape-town Repository record

  14. Predicting Response to Neoadjuvant Chemotherapy in Breast Cancer with Gene Expression and Computational Pathology

    Cope, WeiUniversity of Cambridge2021

    … from routine formalin fixed paraffin embedded (FFPE) histopathological samples. Breast cancers can be classified into distinct groups, 'integrative clusters' (IntClusts), with different outcomes and potentially different response to NAT. The published classification is based on RNA expression …

    cambridge Repository record

  15. Exploration of Nuclear Receptor Activity and siRNA-Derived Phenotypes as Therapeutics in Non-Small Cell Lung Cancer

    Carstens, Ryan Murray2017

    … of mRNA expression signature assessment from FFPE specimens. The platform was used to assess NR/CoReg expression levels across a 500+ sample FFPE dataset. Categorical NR/CoReg downregulation upon tumor progression as well as survival benefits for patients retaining a non-pathological NR/CoReg …

    utswmed Repository record

  16. microRNAs in Uveal Melanoma: in vivo findings and computational analysis

    FALLICO, MATTEO ROBERTOUniversità degli studi di Catania2020

    … and Formalin-Fixed Paraffin-Embedded (FFPE) UM samples were analyzed to assess the expression levels of the three miRNAs under investigation. Droplet digital PCR (ddPCR) was used for both liquid biopsy and tissue samples. A novel computational analysis for the three selected miRNAs was …

    catania Repository record

  17. Determination of the Feasibility of Using Cell-Free DNA (cfDNA) in the Q-Clamp KRAS Codon Specific Mutation Test

    Garcia, Manuel2018

    … using cell-free extracted DNA (cfDNA) instead of FFPE extracted DNA. The results of the experiment showed a clear difference in the Ct’s between the mutant and internal control. Since the delta Ct’s can be discriminated, the assay showed a positive feasibility of using a cfDNA in the Q-Clamp ® …

    dominican Repository record

  18. Oral and Oropharyngeal Cancer - Aspects on Epidemiology and Prognostic Markers

    Annertz, KarinLund University, Faculty of Medicine, Otorhinolaryngology (Lund)2014

    … embedded tissue from their diagnostic biopsies (FFPE), again no survival difference was found. The rate of inconsistent results between SMC and FFPE was 27%. In a set of 55 OOPHSCC, alpha B-crystallin was found to be a strong independent prognostic marker for poor prognosis in oral SCC, but not …

    lund Repository record

  19. Investigating the relationship between miRNA expression and epithelial mesenchymal transition in colorectal cancer

    Jaca, AnelisaDivision of Anatomical Pathology2016

    … HNPCC cases) Formalin Fixed Paraffin Embedded (FFPE) tissue blocks and their corresponding H&E slides were collected from the archives of the Division of Anatomical Pathology at the University of Cape Town. Subsequently, the FFPE tissue blocks were sectioned at 3μm and IHC analysis of 4 EMT …

    cape-town Repository record

  20. DEEP LEARNING ALGORITHM FOR MOLECULAR CLASSIFICATION OF ENDOMETRIAL CANCER FROM WHOLE SLIDE HISTOPATHOLOGY IMAGES

    FRASCARELLI, CHIARAUniversità degli Studi di Milano2026

    … From an initial cohort of 1,362 cases, 230 FFPE WSIs were selected and annotated to train three sequential binary classifiers (POLEmut vs non-POLE, MMRd vs non-MMRd, p53-abn vs NSMP), forming a hierarchical, clinically aligned architecture. Prediction heatmaps were generated to enhance …

    milano Repository record

Page 1 of 3