Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 43 for “"FACS analysis"”.
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Homing of 111In-Labeled Bone Marrow Mesenchymal Stem Cells in Acute Brain Trauma Model
… Bq/cell) of 111In-tropolone, and growth curve analysis, fluorescent activated cell sorter (FACS) analysis after staining with 5-bromo-2-deoxy-uridine (BrdU), and microscopic evaluation after 5-bromo-4-chloro-3-indolyl-D-galactopyranoside (X-gal) staining were performed until the 14th day. FACS …
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Characterisation of CIP29 : a potential target of the eukaryotic DNA damage response
… with the defect in the C9 cells more pronounced. FACS analysis revealed a slightly reduced proportion of the C60 cells in the S and G2/M phases of the cell cycle compared to wild-type cells, which could account for the slightly reduced proliferation rate seen in these cells. FACS analysis …
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Down-modulation of the apoptosis receptor Fas and the EGF receptor by the adenovirus E3/10.4-14.5K proteins requires the concerted action of two distinct transport signals
… directed transport in clathrin-coated vesicles. FACS analysis of stable E3-transfectants expressing 10.4-14.5K mutant proteins revealed that residues contained within these putative transport motifs were essential for down-regulation of Fas and the EGFR in vivo. Receptor expression was restored …
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Molecular Studies involving the Rev Protein of Caprine Arthritis Encephalitis Virus and Visna Virus.
… (Visna Virus RRE). To test these possibilities FACS analysis, RNA binding assays, cotransfections, and SELEX were done. The results indicated that Rev is not acutely toxic to cells, inhibition of Rev activity could not be achieved by making a better binder or through expression of exogenous …
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Verlaufsuntersuchung zellulärer Immunparameter bei schizophrenen Patienten
… gd, CD11a, CD11b and CD49d) was measured by FACS analysis. 20 healthy volunteers served as controls. In the acute state of psychosis a significant elevation of the B-lymphocyte fraction was observed, while the percentage of T-lymphocytes was decreased. These values levelled to those of the …
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세포자멸사 촉진분자를 이용한 암 치료용 단백질치료제의 개발
… 구축 - 8 E. Transfection - 9 F. Western blot analysis - 10 G. Fluorescence activated cell sorter(FACS) analysis - 11 H. co-Immunoprecipitation analysis - 11 I. Confocal microscopy - 13 Ⅲ. 결 과 - 14 A. 돌연변이 Bcl-xL(5A) 단백질의 세포자멸사 촉진 효과 분 석 - 14 B. Etoposide 농도별 돌연변이 Bcl-xL(5A) 단백질의 세포자멸사 촉진 효과 분석 …
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Development of methods for the tissue engineering of cardiac valves using mesenchymal stem cells
… of the human MSC (hMSC) was then examined by FACS analysis. A previously developed decellularised porcine aortic valve matrix was biochemically characterised to ensure that the major matrix components had not been removed by the decellularisation procedure. Furthermore the biocompatibility of …
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A Comparison of Cultured Human Dermal Fibroblasts Derived from Terminal and Vellus Hair Bearing Skin. Differences in the expression of inhibitors of apoptosis proteins, oestrogen receptors, and responses to oestradiol under normal and wound induced conditions
… DF(T) and DF(V) cultures was compared by FACS analysis and mRNA and protein expression of Apollon, cIAP2, NAIP and XIAP and their antagonists DIABLO and Xaf1 analysed by qRT-PCR and immunocytochemistry in unwounded and mechanically wounded fibroblast cultures. Differences in proliferation, …
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Determination of Thymic Epithelial Cell Composition and Proliferation During The Perinatal to Adult Transition
… TEC (mTEC) are expanded in the K5.D1 thymus, FACs analysis revealed a marked increase in a novel UEA-1<sup>int</sup> Sca-1- TEC subset, which is not readily classified as belonging to either the cTEC or mTEC lineage. In addition, low level expression of MHC class II and high-level expression …
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caffeine에 의한 HGF-induced G2 delay 촉진효과 확인 및 기전분석
… significantly. Same observation was obtained by FACS analysis and mitotic index study. Increased G2 delay by caffeine was also accompanied with the delay in cdk1 activation and in cyclin B nuclear localization. Western blot analysis also showed delayed degradation of cyclin A and B. ATM/ATR …
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Production and Characterization of an Human Monoclonal Antibody IgG to TRAIL Receptor 2
… 통해 확인하였다. MTT assay, Annexin V-FITC/PI staining, FACS analysis, sub-G1 분석을 통해 DR5-18 IgG가 림프 암세포 주 Jurkat 에서 apoptotic cell death를 유도하는 것을 확인 하였다. DR5-18 IgG는 Fc 분절을 가지고 있으므로 in vitro 상에서 IgG 단독 처리에 비해 보체와 함께 처리하였을 때 암세포 치사율이 더 높아짐을 확인하였다. 따라서 DR5-18 IgG는 성공적으로 만들어졌음을 확인하였고, 추후 in vivo 실험을 통한 암 …
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Beta 3 integrins : negative regulators of angiogenesis
… by immuno-fluorescence detection and FACS analysis and expressed specific antigens including PECAM-1, ICAM-1, ICAM-2, VCAM-1 and VE-cadherin. Using this method, cells from wild-type and beta 3-integrin-deficient animals were purified and used to determine the specificity of a novel …
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The role of anchorage in cell cycle control
… p27 curbs cell-cycle progression in these cells. FACS analysis showed that surprisingly, these cells do not undergo a robust checkpoint arrest, but instead stall throughout the cell cycle, showing abnormal DNA replication. Further investigation by metaphase spread showed the accumulation of …
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Investigating the role of NSMCE2 in cell cycle progression and genome maintenance
… defects and slowed S phase progression by FACS analysis, with the defect exacerbated in the ligase-dead cells. The remainder of this project investigated the cause of these defects, determining that it is not due to the activation of the replication checkpoint, although the ligase-dead cell …
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Molecular defects in B lymphocytes from patients with CVID- syndrome
… of CVID lymph nodes by immunohistology and FACS showed in three CVID patients numerous enlarged follicles with mostly well developed mantle zones. The follicles contained high percentages of activated and proliferating germinal center B cells, but plasma cells were absent. Analyses of CD27 …
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Biochemical and genetic analysis of the adaptor protein SH3P7 : insights from a newly generated knockout mouse
… fatal disease at 3-4 months of age. Initial FACS-analysis of the cellular composition of the spleen show drastic reduction of T1 B cells which might be interpreted as defect in B cell development or maturation. Symptoms such as progressive paralysis implicate a neuronal disease in male …
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HCV Core Protein affects Lipid Metabolism in a Genotype-Dependent Manner
… lipid regulation in a quantitative manner. Using FACS analysis, and immunofluorescence analysis by using confocal microscope, we found that gt3a core protein induces larger lipid droplet formation, although the quantity of lipids remains similar to that induced by gt1a core protein. We then …
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The Role of the Stress Response Gene Gadd45b in Senescence
… at the G2/M phase of cell cycle. Furthermore, FACS analysis of Gadd45b null MEFs showed less phospho-histone H3-positive cells compared to wild type MEFs indicating that Gadd45b null MEFs are arrested in G2 phase rather than M phase. Interestingly, other stressors such as sub-lethal H2O2 and UV …
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HCV Core Protein affects Lipid Metabolism in a Genotype-Dependent Manner
… lipid regulation in a quantitative manner. Using FACS analysis, and immunofluorescence analysis by using confocal microscope, we found that gt3a core protein induces larger lipid droplet formation, although the quantity of lipids remains similar to that induced by gt1a core protein. We then …
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Role of endoplasmic reticulum stress in neutrophils during tumour initiation
… the Feng lab employed single-cell RNA sequencing analysis to capture all neutrophils in preneoplastic cell-carrying larvae to compare that with neutrophils from control larvae. This revealed a list of upregulated genes linked to endoplasmic reticulum (ER) stress. I hypothesize a potential …
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