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Showing 1 to 20 of 263 for “"FACS"”.

  1. Analysis of a novel cytotoxic drug induced TRAIL-R2 signalling complex using a new FACS-based Smac/DIABLO release assay

    Arora, JigyasaUniversity of Essex2014

    … impaired in DR5-silenced cells, we developed a FACS based Smac/DIABLO release assay in 5-Flurouracil (5-FU) stimulated HCT116 cancer cells. Using this assay we found that DR5 knock-down cells show a markedly reduced Smac/DIABLO release, which corresponds to decreased mitochondrialmembrane …

    essex Repository record

  2. Long-Term Hypoxia Regulates Cortisol Biosynthesis in the Ovine Fetal Adrenal Cortex

    Vargas, Vladimir Enrique

    … Dispersed fetal adrenal cortical cells (FACs) were untreated, treated with ACTH, treated in combination with ACTH precursors (POMC and 22-kDa pro-ACTH), or pre-treated with H-89 and or UO126 followed by ACTH treatment. Following ACTH treatment, cortisol biosynthesis increased in both …

    loma-linda Repository record

  3. Genomewide Crispr/Cas9 Screen Identifies Network of Protein Complexes That Regulate Trim24

    Patel, Lalit, <p>0000-0002-9064-5560</p>2023

    … using fluorescence activated cell sorting (FACS) to identify regulators of TRIM24. The screen was enabled by two innovations. I engineered cells with an in-frame knock-in of mClover3 to the endogenous copy of TRIM24 to allow fluorescent monitoring of TRIM24 expression by flow cytometry. To …

    uthsc Repository record

  4. Characterization of Dendritic Cells in the Bovine Mammary Gland

    Maxymiv, Nicolas GeorgeVirginia Tech2009

    … tissue. Fluorescence-activated cell sorting (FACS) was used to characterize mammary DC as expressing CD11c, MHC-II, CD205, CD11b, and CD8α. FACS allowed us to distinguish DC (CD14lo) from macrophages (CD14hi). Two DC subsets, CD11a-, CD11alo, were evident in the mammary gland while an …

    vt Repository record

  5. Defining Gene Expression in Normal Human Epidermal Keratinocytes and Melanocytes: A Prerequisite for Understanding Hyperplastic and Neoplastic Pathology

    Billick, Erika de Wyllie

    … "normal" sources: 1) cultured keratinocytes, 2) FACS keratinocytes from dispase-separated epidermis, and 3) laser-capture microdissected (LCM) epidermis. For melanocytes, the precursor cell of melanoma, the attempt was made to isolate a more physiologically relevant sample source than that of the …

    rockefeller Repository record

  6. Etablierung einer Zellkultur von PDL-Fibroblasten aus parodontal erkranktem Zahnhalteapparat des Menschen

    Entorf, Anna Maria2012

    … Analyse wird unter Verwendung der Methoden des FACS und der real-time (RT)-PCR durchgeführt. Die Ergebnisse zeigen in der real-time (RT)-PCR eine Herunterregulation von Kollagen Typ I und Runx2 im Vergleich zu gesunden PDL-Zellen. Eine unveränderte Expression stellt sich für CD13 und CD73 als …

    goettingen Repository record

  7. Humanisierung von Maus-Hybridomantikörpern

    Lüttgau, Sandra MarenUniversität Heidelberg

    … Bildung verringert wird. Dafür wurde mittels FACS zunächst ein stabiler Subklon HEA125-1 etabliert, dessen Zellen verglichen mit HEA125 ca. 30% mehr Antikörper auf ihrer Oberfläche exprimieren. Damit können sehr einfach per FACS solche Zellen isoliert werden, die veränderte Antikörper …

    heid-diss Repository record

  8. Automatic real-time facial expression recognition for signed language translation

    Whitehill, Jacob RichardUniversity of the Western Cape2006

    … We also developed a system for detecting FACS action units based on Haar features and the Adaboost boosting algorithm. This method achieves equally high recognition accuracy for certain AUs but operates two orders of magnitude more quickly than the Gabor+SVM approach. Finally, we developed …

    western-cape Repository record

  9. A Novel Click Chemistry-Based Method to Detect Hypoxic Tumour Cells and Characterise Their Gene Expression

    Hou, ALResearchSpace@Auckland2016

    … SN33267) by fluorescenceactivated cell sorting (FACS) according to the cellular fluorescence intensity in every human cancer line tested (N=4) without prior cell fixation and permeabilisation. The RNA extracted from FACS-sorted fractions was of sufficient quality (RIN>6) for measurement of gene …

    auckland-ms Repository record

  10. FACIAL EXPRESSION DISCRIMINATES BETWEEN PAIN AND ABSENCE OF PAIN IN THE NON-COMMUNICATIVE, CRITICALLY ILL ADULT PATIENT

    Arif-Rahu, Mamoona2015

    … video using the Facial Action Coding System (FACS) over a 30 second time period for each phase. Pain scores were calculated from FACS action units (AU) following Prkachin and Solomon metric. RESULTS: Fourteen facial action units were associated with pain response and found to occur more …

    vcu Repository record

  11. Inexpensive technologies enabling widespread utilization of image-predicated cell sorting

    Kovač, Joseph (Joseph R.)Massachusetts Institute of Technology2010

    … technology, fluorescence-activated cell sorting (FACS), offers high throughput and sorts predicated on a wide range of phenotypes that can be conveyed through average cellular fluorescence or light scattering levels. However, FACS cannot detect a wide range of phenotypes easily observable through …

    mit Repository record

  12. Long-Term Hypoxia Alters Ovine Fetal Adrenal eNOS and Cortisol Biosynthesis

    Newby, Elizabeth Anne

    … peNOS in LTH fetal adrenocortical cells (FACs); inhibition of the PI3K/Akt pathway resulted in reduced peNOS and enhanced cortisol production in response to ACTH in LTH FACs. Defining the regulatory role of these pathways will enhance our understanding of how these adaptations to LTH …

    loma-linda Repository record

  13. Soft matter engineering for cellular bioelectronic interfaces

    Denduluri, Akhila JyothyUniversity of Cambridge2022

    … known as photosynthetic-organism directed DE-FACS (podDE-FACS), enables rapid in- vestigation of cyanobacteria, microbes with electrogenic capabilities widely used as the biological source of anodic electrons in the development of renewable power-generation devices, known as biophotovoltaics …

    cambridge Repository record

  14. The Nature of Word Finding Abilities Following Traumatic Brain Injury

    Murphy, April D.

    … Assessment of Communication Skill for Adults (FACS) were administered to each subject. The TAWF was intended to give information regarding word finding in a number of contexts while the FACS was designed to give a measure of functional communication for each subject. Fifty-four percent (N=7) of …

    mo-state Repository record

  15. Application of Automated Facial Expression Analysis and Facial Action Coding System to Assess Affective Response to Consumer Products

    Clark, Elizabeth A.Virginia Tech2020

    … that focused on the Facial Action Coding System (FACS) and its use to investigate consumer affect and characterize human emotional response to product-based stimuli, which revealed inconsistencies in how FACS is carried out as well as how emotional response is inferred from Action Unit (AU) …

    vt Repository record

  16. Granzyme B- und Perforin-positive Lymphozyten in der bronchoalveolären Lavage und dem perpheren Blut bei Patienten mit Asthma bronchiale

    Abd-el-Ghani, Steffen

    … mit Antikörper markiert. Gemessen wurde mittels FACS. Weiterhin wurden die Veränderungen nach Kultivierung oder Stimulation mit PMA und Ca-Ionophore bzw mit anti-CD3 bestimmt.

    freiburg-diss Repository record

  17. 세포자멸사 촉진분자를 이용한 암 치료용 단백질치료제의 개발

    민, 지영2011

    … - 10 G. Fluorescence activated cell sorter(FACS) analysis - 11 H. co-Immunoprecipitation analysis - 11 I. Confocal microscopy - 13 Ⅲ. 결 과 - 14 A. 돌연변이 Bcl-xL(5A) 단백질의 세포자멸사 촉진 효과 분 석 - 14 B. Etoposide 농도별 돌연변이 Bcl-xL(5A) 단백질의 세포자멸사 촉진 효과 분석 - 15 C. 다양한 2차 자극에 대한 세포자멸사 촉진 효과 분석 - 16 D. 다양한 …

    ajou Repository record

  18. Homing of 111In-Labeled Bone Marrow Mesenchymal Stem Cells in Acute Brain Trauma Model

    박, 복남2011

    … analysis, fluorescent activated cell sorter (FACS) analysis after staining with 5-bromo-2-deoxy-uridine (BrdU), and microscopic evaluation after 5-bromo-4-chloro-3-indolyl-D-galactopyranoside (X-gal) staining were performed until the 14th day. FACS analysis after staining with Annexin V- …

    ajou Repository record

  19. Proteinase 3 and Neutrophil Apoptosis in ANCA-Associated Systemic Vasculitis

    AbdGawad, MohamedLund University2010

    … and membrane expression was measured by FACS. Plasma PR3, pro-PR3 and cytokines were measured by ELISA. Neutrophil apoptosis was measured by FACS after 20h in vitro culture. Expression of pro/anti-apoptotic factors and transcription factors C/EBP-α, C/EBP-β and PU.1 was measured by …

    lund Repository record

  20. Einfluß von Steroidhormonen auf die Endothelzellproliferation und die Expression von Wachstumsfaktoren und ihren Rezeptoren

    Schürle, Charlotte

    … kultiviert. Die Endothelzellen wurden mit FACS-Analysen identifiziert. Durch immunzytochemische Untersuchungen wurde die Existenz von Östrogen- und Progesteronrezeptoren gesichert. Im Rahmen der Proliferationsstudien wurden die mit Steroiden inkubierten Zellen untersucht und die Zellzahl …

    freiburg-diss Repository record

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