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Showing 1 to 20 of 20 for “"F420"”.
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Biophysical and structural studies of novel F420-dependent oxidoreductases in Mycobacterium tuberculosis
… bind tightly to the unusual flavin coenzyme F420 by isothermal calorimetry (ITC), saturation transfer difference (STD) NMR, and X-ray crystallography. Although recent studies have drawn attention to F420 due to its role in activating the promising anti-tuberculosis drug PA-824, little is …
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A biochemical and physiological characterization of coenzyme F420-reducing hydrogenase from Methanobacterium formicicum
The coenzyme F₄₂₀-reducing hydrogenase of Methanobacterium formicicum was purified 87-fold to electrophoretic homogeneity. The enzyme formed aggregates (1,000 kd) of a coenzyme F₄₂₀-active monomer (109 kd) composed of 1 each of a, β, and γ subunits (43.6, 36.7, xy and 28.8 kd, respectively). It …
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The Structure of Coenzyme F420, a Novel Electron Carrier Isolated From Methanobacterium Strain m.o.h
Made available in DSpace on 2014-12-14T07:14:45Z (GMT). No. of bitstreams: 1 7811229.pdf: 5632154 bytes, checksum: 7e8c00fb0ed2cfb07179155d42128a93 (MD5) Previous issue date: 1978
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Redox-sensitive enzyme activities involved in methyl group transfer and coenzyme F420 conversion in Methanobacterium thermoautotrophicum
Contains fulltext : mmubn000001_111343151.pdf (Publisher’s version ) (Open Access)
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Stereoselectivity, kinetics, and engineering of flavin/deazaflavin oxidoreductases for use in industrial biocatalysis
… Due to their unusual deazaflavin cofactor, F420, the flavin/deazaflavin oxidoreductase (FDOR) family have attracted attention as potential biocatalysts. A low-potential obligate hydride donor with chemistry more akin to nicotinamides than conventional flavins, F420 plays a crucial role in …
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Stereoselectivity, kinetics, and engineering of flavin/deazaflavin oxidoreductases for use in industrial biocatalysis
… Due to their unusual deazaflavin cofactor, F420, the flavin/deazaflavin oxidoreductase (FDOR) family have attracted attention as potential biocatalysts. A low-potential obligate hydride donor with chemistry more akin to nicotinamides than conventional flavins, F420 plays a crucial role in …
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Understanding the physiological role of cofactor F 420 in mycobacterium
The unique deazaflavin cofactor F420 occurs widely among mycobacterial species, including pathogenic ones. It is also involved in the activation of the antitubercular prodrug PA-824. We created a KO-mutant of F420 in M. bovis BCG to study the physiological significance of this cofactor in …
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Novel Electron Transfer Systems in Hyperthermophilic Methanogenic and Anaerobic Methanotrophic Archaea: F420-dependent Nitrite Reductase and [Fe-S] Cluster Assembling Thioredoxin
… linked to the organism's survival strategies, an F420-dependent sulfite reductase (Fsr), deazaflavin-dependent flavin-containing thioredoxin reductase (DFTR), and a thioredoxin (Trx) homolog with a new function. These proteins are involved in redox reactions, and the research presented here deals …
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Mycobacterium tuberculosis strains in New Zealand: phylogeny and structural biology
… to the high transmissibility of this strain. The F420-dependent oxidoreductase Rv2893 harbours a Rangipo-specific G72S mutation encoded by a nsSNP. H37Rv and Rangipo Rv2893 structures were solved and show the effect of this G72S mutation. Binding of the F420 cofactor was confirmed and …
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Mechanisms of proton translocation in Methanosarcina mazei Gö1
… Methanosarcina mazei Gö1 analysiert werden: Die F420H2:Heterodisulfid-Oxidoreduktase, die H2:Heterodisulfid-Oxidoreduktase sowie eine protonentranslozierende Pyrophosphatase.Es konnte gezeigt werden, dass 2-Hydroxyphenazin den Elektronentransport zwischen den Schlüsselenzymen der …
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Charakterisierung des molekularen Mechanismus der Stabilisierung des Peptidtransporters TAP durch das ER-Glykoprotein Tapasin
… Tpn bestehend aus den konservierten Aminosäuren F420, F424, G428, K431 und W435 ausgeübt wird. Der kombinierte Austausch von E414 im CP und des räumlichen Motivs erbrachte einen kompleten Verlust der Tpn-vermittelten TAP2-Stabilisierung und zeigte damit eine kooperative Wirkung dieser …
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Biochemical characterization of a novel iron-sulfur flavoprotein from Methanosarcina thermophila strain TM-1
… and the designated electron carriers (NAD, NADP, F420, and 2-hydroxyphenazine) were used in an attempt to determine the electron acceptor for Isf. Isf was unable to reduce any of these compounds. Furthermore, 2-hydroxyphenazine competed with Isf to accept electrons from ferredoxin A indicating …
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Hydrogenase utilization and regulation in species of methanosarcina
… expression based on the redox state of coenzyme F420. In Chapter 3, I explored the regulation of hydrogenases in Methanosarcina acetivorans, a species that is incapable of metabolizing H2, yet encodes hydrogenases with high similarity to the active enzymes found in M. barkeri. Despite the lack of …
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Charakterisierung und Kristallisation der Elektronen-einspeisenden Module der F<sub>420</sub>H<sub>2</sub>-Dehydrogenase
Die F420H2: Chinon Oxidoreduktase aus dem hyperthermophilen sulfatreduzierenden Archaeon Archaeoglobus fulgidus und die F420H2: Phenazin Oxidoreduktase des methanogenen Archaeons Methanosarcina spec. sind initiale Enzyme eines membrangebundenen Elektronentransportsystems und an der …
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Mechanistic Insights into MftR-Dependent Regulation of the Redox Cofactor Mycofactocin
… example, in <em>Actinobacteria</em>, coenzyme F420 is commonly used in place of flavin mononucleotide in enzymes associated with carbon fixation and oxidation of secondary alcohols. The importance of niche cofactors has long been recognized however, detailed understanding about their …
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Evolution of targets at the host-pathogen interface
… presence of first-line antibiotics) and cofactor F420-binding proteins (which bind to a microorganism-specific cofactor, regulate various processes, and can be targeted with one drug) primarily using in silico biochemical methodologies. Chapter 4: SARS-CoV-2 proteome modelling and drug target …
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What does the analysis of copper-alloy brooches reveal about the social and economic landscapes of Early Anglo-Saxon Cambridgeshire and Suffolk?
This thesis examines the social and economic landscape of the Early Anglo-Saxon period by analysing copper-alloy brooches using X-ray fluorescence spectroscopy. Two clusters of cemeteries, one in Cambridgeshire and the other in Suffolk, were chosen to have their brooches analysed. The results of my …
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Sulfite reductase and thioredoxin in oxidative stress responses of methanogenic archaea
… use an unusual sulfite reductase, named F420-dependent sulfite reductase (Fsr), for the detoxification of SO32- that is produced outside the cell from a reaction between oxygen and sulfide. This reaction occurred during early oxygenation of Earth and continues to occur in deep-sea …
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Analysis of hydrogen metabolism in methanosarcina barkeri fusaro
Made available in DSpace on 2010-05-14T20:51:15Z (GMT). No. of bitstreams: 3 Kulkarni_Gargi.pdf: 26285517 bytes, checksum: 09a83a385fb35766510a0bf93e3d07d8 (MD5) 1_Kulkarni_Gargi.pdf: 26264601 bytes, checksum: bedca9c1fe77d00d0cde15df38d30446 (MD5) license.txt: 4064 bytes, checksum: …