Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 41 for “"Förster Resonance Energy Transfer (FRET)"”.
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Förster resonance energy transfer (FRET)-based nanophotonics using DNA origami structures
… The elements of nanoscale photonic devices can transfer excitation energy non-radiatively from an excited donor molecule to an acceptor molecule by Förster resonance energy transfer (FRET). The efficiency of this energy transfer is highly dependent on the donor-acceptor distance. Hence, in these …
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Lanthanides and quantum dots : time-resolved laser spectroscopy of biochemical Förster Resonance Energy Transfer (FRET) systems
Förster Resonance Energy Transfer (FRET) plays an important role for biochemical applications such as DNA sequencing, intracellular protein-protein interactions, molecular binding studies, in vitro diagnostics and many others. For qualitative and quantitative analysis, FRET systems are usually …
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Structure and Dynamic Studies of the Nuclear Pore Complex at the Single-Molecule Level
… techniques including electron microscopy, Förster resonance energy transfer (FRET), and high-resolution near-field scanning optical microscopy (NSOM) are utilized to specifically locate vault ribonucleoproteins to NPCs. This interaction, along with several other results, strongly suggests …
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Towards The Development Of Intrinsically Fluorescent Unnatural Amino Acids For In Vivo Incorporation Into Proteins
… involving fluorescence polarization (FP) and Förster resonance energy transfer (FRET) measurements, but to date was limited to solely E. coli expressed proteins. This thesis work will continue to explore Acd’s use in in vitro measurements from E. coli expressed proteins. Mainly this will focus …
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Advancing Photoacoustic Imaging Technology with Compact Source, Fluorescence Co-registration, Spectral Encoding, Matrix Detection and FRET
… exciting and detecting photoacoustic signals. Förster resonance energy transfer (FRET) imaging in deep biological tissue using photoacoustic techniques is also explored.</p><p>This first part of my dissertation presents some novel photoacoustic excitation and detection technologies implemented …
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Poly(LA-co-TMCC)-graft-PEG Self-assembled Polymeric Nanoparticles for Targeted Drug Delivery
… serum protein solutions. By encapsulating Förster resonance energy transfer (FRET) pairs and following their release by fluorescence, these micelles demonstrated strong thermodynamic and kinetic stability in the presence of serum. By incorporating functional groups (azide or furan) on the …
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The Tansmembrane Associations of HIV-1 Vpu and its Human Targets
… fluorophore labeled TM domain peptides we used Förster resonance energy transfer (FRET) to characterize the interactions of Vpu with itself as well as its human targets. Our data show target TM domains compete for interaction with Vpu, suggesting that all targets bind the same helical face, also …
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Developing a co-culture model of ovarian cancer to identify immunotherapies that can overcome TGF-β-induced immunosuppression
… cells were transduced with a GzmB-cleavable Förster resonance energy transfer (FRET) reporter and either the chicken ovalbumin (OVA) epitope or scrambled (SCR) epitope control. Co-culturing OVA cells with OVA-specific OT-1 T cells resulted in decreased FRET-positive cells, indicating OT-1 T …
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The synthesis and study of novel squaramide-based anion sensors
… substituted MSQ which is capable of carrying out Förster Resonance Energy Transfer (FRET) are also discussed in this chapter. In addition, the anion binding properties of successfully synthesized MSQs are studied. Chapter 4 gives an overall conclusion of the entire work carried out in this …
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Supramolecular quantum dot-porphyrin assemblies for biological oxygen sensing
… and porphyrin absorption results in efficient Förster resonance energy transfer (FRET) for signal transduction in these sensors. Porphyrins with meso pyridyl substituents bind to the surface of the QD to produce self-assembled nanosensors. Since these macrocycles are sensitive in the 0-160 torr …
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The Role of Pag1 In The Activated B-Cell Subtype of Diffuse Large B-Cell Lymphoma
… mediators of BCR activation through the use of a Förster resonance energy transfer (FRET) based assay in living cells, measuring time-course, dose-response, and kinetic assays by flow cytometry. Together, these studies lay the groundwork for further exploration into the role of PAG1 in B cell …
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Understanding the Role of Anisotropy on the Optoelectronic and Colloidal Properties of Quantum Rods for Programmable Self-Assembly with DNA Origami
… to align these quantum rods on DNA origami for energy transfer applications in biocompatible systems. First, CdSe of systematically varied aspect ratios and emission colors, as well as core/shell CdSe/CdS of different microstructures were synthesized and characterized. Next, the surface ligand …
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A FRET Investigation into Molecular Mechanisms of Cardiac Troponin Activation in Reconstituted Thin Filaments
… inhibition is not well understood. A multi-site Förster resonance energy transfer (FRET) assay in cardiac Tn in reconstituted thin filaments was used to investigate the Ca2+-dependent structure and dynamics of the SABS, and show current theories behind Tn activation are biased using structures …
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Exploration of helicase mechanisms at single molecule level: answering decades old questions and bioengineering super proteins
… is central to life. We used single molecule Förster resonance energy transfer (FRET) and optical tweezers force spectroscopy to study translocation and unwinding mechanism of Escherichia coli Rep, a model helicase with 3’5’ motor translocation activity on single‐stranded DNA (ssDNA) fueled …
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Exploration of helicase mechanisms at single molecule level: answering decades old questions and bioengineering super proteins
… is central to life. We used single molecule Förster resonance energy transfer (FRET) and optical tweezers force spectroscopy to study translocation and unwinding mechanism of Escherichia coli Rep, a model helicase with 3’5’ motor translocation activity on single‐stranded DNA (ssDNA) fueled …
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Advancing fluorescence lifetime microscopy for quantitative bioimaging
… with sub-cellular resolution. Concurrently, energy levels in the mitochondria were monitored using fluorescent sensors based on Förster resonance energy transfer (FRET). A published approach of delivering exogenous mitochondria to cells was applied to rescue some of the defects seen in the …
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Development of Methods for Structural Characterization of Pantoea stewartii Quorum-Sensing Regulator EsaR
… Using HMGE, amino acid substitutions and Förster resonance energy transfer (FRET), experimental methods were designed for determining the dimerization interface of EsaR and for testing the hypothesis that EsaR undergoes a conformational shift when presented with the AHL ligand. To identify …
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Developing and Utilizing Gas-phase Laser Spectroscopy Techniques to Study the Effect of Desolvation on Molecules
… 3 and 4 showcase the development in the use of Förster resonance energy transfer (FRET) as a tool for structural analysis of gas-phase proteins, focusing on ubiquitin ions as a model system. FRET efficiencies of the ions suggest that their conformation can remain similar to what is known in …
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Light in Living Systems: Ultra-Weak Photon Emission and Fluorescence Lifetime Imaging of Cellular Dynamics
… into the fluorescence regime, employing dual dye Förster resonance energy transfer (FRET)-fluorescence lifetime imaging microscopy (FLIM) to probe mitochondrial nano-organisation. Dual staining with MitoTracker^(TM) Green FM (MTG) (donor) and MitoTracker^(TM) Orange (MTO) (acceptor) produced …
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An optical proteomic profile of the HER2 family in breast tumours and the effect of exposure to tyrosine kinase inhibitors
… and protein-protein interactions by measuring Förster resonance energy transfer (FRET). Where fluorophores are located within nanometer proximity, FRET occurs, thus allowing quantification of protein-protein interactions. This novel technology is used alongside traditional biochemical methods, …
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