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Showing 1 to 20 of 72 for “"Förster Resonance Energy Transfer"”.
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Förster resonance energy transfer (FRET)-based nanophotonics using DNA origami structures
… The elements of nanoscale photonic devices can transfer excitation energy non-radiatively from an excited donor molecule to an acceptor molecule by Förster resonance energy transfer (FRET). The efficiency of this energy transfer is highly dependent on the donor-acceptor distance. Hence, in these …
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Conformational Dynamics of the Glutamate Receptors via single Molecule Förster Resonance Energy Transfer
… Herein it describes the use of single molecule Förster resonance energy transfer to observe the bound form of the glutamate receptors and subsequent dynamics. For a complete understanding of the dynamics involved in the conformational landscape, the response of the receptor to a variety of known …
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Lanthanides and quantum dots : time-resolved laser spectroscopy of biochemical Förster Resonance Energy Transfer (FRET) systems
Förster Resonance Energy Transfer (FRET) plays an important role for biochemical applications such as DNA sequencing, intracellular protein-protein interactions, molecular binding studies, in vitro diagnostics and many others. For qualitative and quantitative analysis, FRET systems are usually …
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Development of an in-cell förster resonance energy transfer technique to study protein structure inside living cells
… this goal, we will apply the fluorescence resonance energy transfer (FRET) technique to the specifically labeled proteins inside the cells. Our rationale is to specifically label the protein(s) of interest in the test tube with a small molecule fluorophore and then deliver the labeled …
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Single Molecule Förster Resonance Energy Transfer and Super Resolution Force Spectroscopy Study of Elongation Factor G Evolution, Conformation, and Function
… inhibited tRNA translocation. Single molecule Förster resonance energy transfer (smFRET) experiments determined this inhibition stems from the mutants becoming trapped in a ‘super’-compact conformation on the ribosome. The Thr48 mutations were modeled using AlphaFold predictions and MSAs. This …
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Measurement of internal movements of the Eschericia coli ribosome using Förster Resonance Energy Transfer & microsecond, continuous-flow turbulent mixing in microfabricated devices
Submitted by Meng Tao (mengtao2@illinois.edu) on 2012-10-23T20:18:12Z No. of bitstreams: 1 Majumdar_Zigurts.pdf: 5873384 bytes, checksum: 3da545408d4102cc9f2957184b32ebb7 (MD5)
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Förster Resonance Energy Transfer-Based Quantification of D-2-Hydroxyglutarate & Locked Nucleic Acid Loop-Mediated Isothermal Amplification Mediated Detection of IDH1-R132 Single Nucleotide Variants in Glioma
<p>Isocitrate dehydrogenase 1 (<em>IDH1</em>) is a biomarker in gliomas which provides valuable information on patient prognosis and treatment. Targeted therapies including maximal surgical resection and pharmaceutical mutant IDH inhibitors offer life extending benefits; however, currently …
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Agonist-Induced Conformational Changes In The Nmda Receptor
… these issues. Herein, I have conducted Förster Resonance Energy Transfer measurements on individual NMDA receptor molecules to observe their conformational landscape under various conditions. These studies have revealed changes in conformation of the receptor that underlie negative …
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Structure and Dynamic Studies of the Nuclear Pore Complex at the Single-Molecule Level
… techniques including electron microscopy, Förster resonance energy transfer (FRET), and high-resolution near-field scanning optical microscopy (NSOM) are utilized to specifically locate vault ribonucleoproteins to NPCs. This interaction, along with several other results, strongly suggests …
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Characterization of protein induced fluorescence enhancement (PIFE): A label-free protein assay with short distance sensitivity
Single molecule FRET (Förster Resonance Energy Transfer) has been recently widely used for monitoring the interactions between a protein and a nucleic acid. It typically requires a site-specific labeling of the protein, which can be labor-intensive and inefficient. In addition, for common dye …
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Effect of oxidative stress on human telomeric DNA structural dynamics and accessibility
… telomere integrity. Here, we use single molecule Förster Resonance Energy Transfer (smFRET) to characterize the effect of 8OG on the structural dynamics and accessibility of ssTEL. We show that the stable GQ folding observed in ssTEL is disrupted by a single 8OG, generating dynamic conformational …
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Functional Effects of Calcium Regulation of Thin Filaments at Single Particle Resolution
… colocalization, in vitro motility assays, and Förster resonance energy transfer based on fluorescence lifetime imaging (FLIM-FRET). Our results suggest that under physiologically relevant conditions, Tn and Tm binding to Factin is not cooperative and it is not affected by Ca2+. This suggests …
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Resolving Signal Transduction in Complex Biological Environments
… and intracellular domain using single-molecule Förster Resonance Energy Transfer measurements. Furthermore, we disentangle the role of the complex environment around EGFR in its transmembrane signaling by (1) uncovering the specific task of the active components of the plasma membrane; (2) …
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Understanding the activation-deactivation mechanism of glutamate receptor proteins at the single molecule level
… this research, we have utilized single molecule Förster Resonance Energy Transfer (smFRET) spectroscopy to provide a mechanistic understanding of the activation processes that the receptors undergo. We have probed, at a single molecule level, the structural changes explored by the NMDA receptor …
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Towards The Development Of Intrinsically Fluorescent Unnatural Amino Acids For In Vivo Incorporation Into Proteins
… involving fluorescence polarization (FP) and Förster resonance energy transfer (FRET) measurements, but to date was limited to solely E. coli expressed proteins. This thesis work will continue to explore Acd’s use in in vitro measurements from E. coli expressed proteins. Mainly this will focus …
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Unveiling Dna Polymerase Synthesis And Proofreading Activities - One Molecule At A Time
… Christian, et al. developed a single molecule Förster resonance energy transfer (smFRET) approach to monitor the polymerase position on the DNA with single base pair resolution. </p> <p> We have worked to optimize nearly every aspect of that approach and to apply the technique to characterize …
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Advancing Photoacoustic Imaging Technology with Compact Source, Fluorescence Co-registration, Spectral Encoding, Matrix Detection and FRET
… exciting and detecting photoacoustic signals. Förster resonance energy transfer (FRET) imaging in deep biological tissue using photoacoustic techniques is also explored.</p><p>This first part of my dissertation presents some novel photoacoustic excitation and detection technologies implemented …
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Poly(LA-co-TMCC)-graft-PEG Self-assembled Polymeric Nanoparticles for Targeted Drug Delivery
… serum protein solutions. By encapsulating Förster resonance energy transfer (FRET) pairs and following their release by fluorescence, these micelles demonstrated strong thermodynamic and kinetic stability in the presence of serum. By incorporating functional groups (azide or furan) on the …
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Fret-Based Investigations of The Structure-Function Relationships In The Nmda Receptor
… work in this dissertation uses single molecule Förster Resonance Energy Transfer (smFRET) as a means to address that gap. We have examined the question of partial agonism of the NMDA receptor, noting a mechanism of a dynamically graded cleft closure. We have pushed the bounds of the temporal …
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The Tansmembrane Associations of HIV-1 Vpu and its Human Targets
… fluorophore labeled TM domain peptides we used Förster resonance energy transfer (FRET) to characterize the interactions of Vpu with itself as well as its human targets. Our data show target TM domains compete for interaction with Vpu, suggesting that all targets bind the same helical face, also …
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