Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Extrachromosomal DNA"”.
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Development of genetic systems for Clostridia spp. by utilization of extrachromosomal DNA elements
… were 10$\sp6$ and 10$\sp4$ transformants/ug DNA in E. coli and C. perfringens.
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Gene expression from unintegrated HIV-1 is restricted by the SMC5/6 complex
Integration of viral DNA into the host genome is the hallmark of human immunodeficiency virus 1 (HIV-1) infections. However, in early infection, the most abundant viral DNA species are extrachromosomal unintegrated viral genomes, which despite being competent for gene expression, are poorly …
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Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus
… but was possibly due to genetic instability. Two extrachromosomal DNA species were isolated from S. alboflavus, (SAP1 and SAP2). SAP1 was approximately 8 - 10 x 10â ¶ da1tons, and appeared to be linear and heterogenous. SAP2 was 20 - 25 x 10â ¶ daltons and appeared to be covalently closed …
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Plasmids in Clostridium botulinum type A and Clostridium sporogenes
… to agarose gel electrophoresis to detect plasmid DNA. Culture age affected plasmid detection due to changes in the cell density and lysing efficiency. Middle to late log cultures were used throughout the study because they provided optimum plasmid detection. Four out of five C. botulinum type A …
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A Neural Network and Genome Language Model Based Approach to Understanding DNA Replication in Cancer
… identical daughter cells. This process is called DNA replication. DNA replication differs between healthy cells and cancer cells and can be exploited as a therapeutic target. At the same time, altered replication dynamics are critical for understanding cancer onset, evolution and treatment …
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Landscape of Ecdna-Borne Non-Coding Rnas In Glioblastoma
… can occur on a form of non-chromosome DNAs that is often amplified to tens or hundreds of copies, and that can exist in more than half of cancer types. This type of genetic material was termed extrachromosomal DNA (ecDNA). ecDNA appears to be epigenetically highly accessible and …
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Sequence and Functional Structures of the Extrachromosomal rDNA Minichromosome of Physrum Polycephalum
… on multiple copies of linear, palindromic extrachromosomal DNA molecules (rDNA), which have been isolated both as a satellite DNA molecule, and as a "minichromosome" with associated chromatin proteins. The genes coding for the 19 S, 26 S, and 5.8 S rRNAs are transcribed as a single, large …
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Revealing SMC5/6's Dynamic Behavior on Diverse DNA Topologies Through Single-Molecule Methods
… maintains chromosomal stability by coordinating DNA repair processes, promoting successful genome duplication, and silencing viral extrachromosomal DNA. Although these main functionalities have been identified, there is little understanding of how Smc5/6 operates as a versatile molecular machine …
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Dissecting the genomic and epigenomic alterations underlying glioblastoma
… deletions, associated with complex SVs such as extrachromosomal DNA (ecDNA) and chromothripsis. We discovered underappreciated features of ecDNAs in GBM, including a high prevalence of ecDNAs generated via episomal exclusion, and uncovered the extent by which ecDNAs shape tumoural heterogeneity …
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Integrating Epidemiological and Genomic Factors to Inform Outcomes in Barrett’s Oesophagus and Oesophageal Adenocarcinoma
… events such as breakage-fusion-bridges and extrachromosomal DNA did not differ according to OAC phenotype. Importantly, signature 17, which is shown to be preserved across the BO-OAC continuum, was equally enriched among the BO+ve OAC and BO-ve OAC tumours. This thesis presents the first …
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The characterisation of a novel Xanthomonas Bacteriophage
… of 40 and 25.7 kDa, and it has a single-stranded DNA genome. Biological characterisation of the novel bacteriophage indicates that it is a temperate phage which does not cause visible plaque formation on solid media and is not inducible by the external factors, mitomycin C and ultraviolet …