Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Extracellular microenvironment"”.
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Extracellular microenvironment for the regulation of endothelial angiogenic responses
… along with altered biomechanical and cellular microenvironment. Vascular tissue engineering approaches have been realized as a beneficial means of facilitating the treatments for these diabetic conditions. However, these vascular tissue engineering approaches have had limited success due to the …
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Modulation of Chondrogenic and Osteogenic Differentiation of Mesenchymal Stem Cells through Signals in the Extracellular Microenvironment
… In this work, signals present in the native microenvironment were utilized in fabricating polymer/extracellular matrix composite scaffolds to guide chondrogenic and osteogenic differentiation. In an osteochondral defect environment, interactions exist between bone marrow cell populations. …
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Quantitative analysis of 2D and 3D models for epidermal growth factor receptor-dependent cell migration in the context of the extracellular microenvironment
… (EMT) and migrate through a complex tumor microenvironment to successfully metastasize. While the activation of EGFR enhances invasiveness in vivo, it is still unclear which downstream molecular changes caused by EMT contribute to the invasive phenotype and subsequently, how the invasive …
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Nickel Reduces Calcium Dependent Dimerization In Neural Cadherin
… binding of calcium ions to specific sites in the extracellular region. Given the complexity of the cell adhesion microenvironment, we are investigating whether other divalent cations might affect calciumdependent dimerization of neural-(N-) cadherin. The first chapter focuses on studies to …
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Cell culture platforms with cell-cell adhesive ligands for biomedical applications
… mechanical and chemical cues within the extracellular microenvironment, including the surrounding matrix and neighboring cells. Although biomaterials have been engineered to present several adhesion molecules that mimic cell-extracellular matrix interactions, many materials do not …
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Development of orthogonally crosslinked thiol-ene hydrogels for encapsulation of pancreatic beta-cells
… through orthogonal crosslinking, thus mimicking extracellular microenvironment in the pancreatic islets. Finally, in order to provide coated β-cells with an ideal biomechanical microenvironment, it is essential to identify a suitable gel stiffness to support the viability and functions of …
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Engineering Matrix Properties for Stem Cell Culture and Gene Delivery
… chemical properties of hydrogel-based synthetic extracellular matrices (ECMs). First, a synthetic stem cell niche with controlled stiffness and density of cell adhesion peptides was developed for spermatogonial stem cell (SSC) culture (Chapter 2). The synthetic stem cell niche allowed for the …
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Investigating Mechanotransduction and Mechanosensitivity in Mammalian Cells
… forces and mechanical properties of the local microenvironment. Extracellular forces causing cellular deformation can originate from many sources, such as fluid shear stresses arising from interstitial or blood flow, mechanical stretching during breathing or compression during muscle …
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Characterization of peptide release from neuronal cells with microfluidics and mass spectrometry
… of mass-limited samples and the control of the extracellular microenvironment, making it well suited for studying neurons. In this work, we developed several microfluidic devices that can be interfaced to matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) for …
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Heparan sulfate glycosaminoglycan regulation of vasculogenesis
… exists which implicates the role of the extracellular microenvironment in stem cell differentiation. Heparan sulfate glycosaminoglycans (HSGAG) are a crucial part of the dynamic extracellular matrix and have been shown to regulate multiple signaling cascades, including vasculogenic …
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Engineered biomaterials as extracellular microenvironments for guiding cell programming and reprogramming
… biomimetic materials that realize the ‘‘in vivo” microenvironments’ ability to interact with cells. However, not only is designing tailored biomaterials that present multiple signals challenging, but the precise roles of physical and biochemical cues in coordinating cellular processes such as …
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Stiffness controls primary cilia position and length: mechanisms and potential in reversing osteoarthritis phenotypes
… thesis was to determine how the stiffness of the extracellular microenvironment influences cellular mechanosensitivity through the action of, the primary cilium. Mechanical stress and the stiffness of the extracellular matrix are key drivers of tissue development and homeostasis. Aberrant …
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TARGETING REDOX IN AGING AND ALZHEIMER'S DISEASE
… cysteines are the major reducing buffer in the extracellular matrix. Since the relationship of the prominent ROS damage to aging and AD is unclear, we wanted to know whether A) an oxidative redox shift precedes these markers and leads to macromolecular damage, B) age and AD-related changes can …
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A dissection of class I phosphoinositide 3-kinase signalling in mouse embryonic fibroblasts and prostate organoids
Class I PI3Ks are a family (α, β, δ and γ) of ubiquitous lipid kinases that can be activated by cell surface receptors to 3-phosphorylate PI(4,5)P2 (phosphatidylinositol(4,5)-bisphosphate) and generate the signalling lipid PI(3,4,5)P3. The PI(3,4,5)P3 signal then activates a diverse collection of …