Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Extracellular Enzymes"”.
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Biocontrol Systems for Rhizotocnia Disease of Soybean
… 2B-12 indicated the possible involvement of extracellular enzymes in antagonism and biocontrol. Antagonism by cell-free culture filtrate fractions indicated that strain B153-2-2 produced an active metabolite. Endopeptidase, acid protease, and an extracellular proteinase (metalloproteinase) …
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Identification and characterization of genes in antagonism against soybean fungal pathogens by lysobacter enzymogenes
… virguliforme), and in the production of four extracellular enzymes (protease, lipase, cellulase, and chitinase). In addition, the production of the heat stable antifungal factor (HSAF), a fungal specific antibiotic produced by LeC3, was determined in these nine mutants using HPLC and all nine …
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Linking The Functional Diversity Of Ectomycorrhizal Fungal Species To Soil Carbon And The Genetics Of A Foundational Tree Species
… to soils. Ectomycorrhizal fungal species produce extracellular enzymes that may degrade soil carbon and may differ in their ability to produce these extracellular enzymes to break down C in the soil. Additionally, tree genetics may control carbon storage in forests through influences on the …
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Performance Evaluation and Yield Determination of a Full-Scale Biological Aerated Filter
… quantify the activity associated with hydrolytic enzymes in the bulk-liquid. Hydrolytic activity by cell-free extracellular enzymes in the bulk-liquid increased when particle substrate was present. Therefore, it appears that cell-free extracellular enzymes participate in the hydrolytic mechanism …
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Separation of gliadin peptides for investigation of the injurious agent(s) in gluten sensitive enteropathy
… 93) were subjected to in vitro hydrolysis by the extracellular enzymes pepsin, peptidase and pancreatin. Gliadins from one variety (Karl) also were exposed to the intracellular enzymes cathepsins Band D. A reverse phase high performance liquid chromatography (RP-HPLC) method was developed to …
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Potential enzyme activities in soils as affected by perennial cropping and nitrogen rates in central Iowa
… carbon (C), and overall soil health improvement. Extracellular enzymes (EEs), produced mainly by soil microorganisms but also by plant roots, largely mediate soil organic matter (SOM) decomposition. These EEs in soil are known to play a substantial role in maintaining soil health by catalyzing …
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The Effects of Ecological Restoration on Soil Microbial Enzyme Activities and Leaf Litter Decomposition
… and decomposition. The activities of five enzymes: phosphatase, ?-glucosidase, ?-N-acetylglucosadminidase (NAGase), phenol oxidase, and lignin-peroxidase were measured in both soils and on decomposing Quercus falcata leaf litter in unburned, burned, and burned and thinned plots in a mesic …
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Utilization of the Phanerochaete chrysosporium ligninolytic system and the potential for toxaphene degradation
The idiophasic production of nonspecific extracellular enzymes by the Basidiomycete fungus Phanerochaete chrysosporium, referred to as the ligninolytic system, has been reported by many researchers to be capable of degrading a broad spectrum of environmentally persistent compounds including some …
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Gut-associated microbial symbionts of the marsh fiddler crab, Uca pugnax
… microbes benefit the host by contributing extracellular enzymes along the digestive tract. This is the first report of the eccrinid protists, Enteromyces callanassae and Enterobryus sp., inhabiting U. pugnax. The greatest abundances of both bacteria and protists were documented in the host …
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Phenotypic variation in Photorhabdus temperata K122
… and does not produce pigment, bioluminescence, extracellular enzymes, antibiotics and displays a different colony morphology to the primary variant. Study of the phenotypic switch undergone by Photorhabdus has identified two signalling pathways which play a role in the regulation of phenotypic …
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Exploration of Mycoremediation Candidate Screening: Uptake and Tolerance of Bismuth by Native Fungi
… have an array of surface functional groups and extracellular enzymes capable of detoxifying or sequestering pollutants. Furthermore, bioremediation is inexpensive and eco-friendly, with the caveat that potential ecosystemic interactions should be considered with bioremediation candidates. Fungi …
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The relevance of adenylate levels and adenylate converting enzymes on metabolism and development of potato (Solanum tuberosum L.) tubers
… in the surface-to-volume ratio. To identify extracellular enzymes involved in adenylate conversion, green fluorescent protein and activity localization studies in potato tissue were carried out. It was found that extracellular ATP is imported into the cell by an apoplastic enzyme complement …
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The Effect of Environment on Virulence Factors and Resistance of Mucoid Strains of Pseudomonas Aeruginosa
… resulted in enhanced production of the extracellular products, while magnesium limited cells produced the least amounts. M7 showed lower amounts of extracellular enzymes in culture supernatants but higher levels of cell bound enzymes. The sensitivity to polymyxin was markedly affected by …
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Functional Characterization of Proteins Involved in Starch Utilization by Bacteroides Thetaiotaomicron
… them to smaller fragments using cell-associated enzymes. This is in contrast to many other systems which use extracellular enzymes to hydrolyze the polysaccharide to digestible fragments. The Salyers lab has used starch utilization by B. thetaiotaomicron as a model to test this hypothesis. In …
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Molecular characterization of the starch utilitization system of Bacteroides thetaiotaomicron
… divide-and-conquer approach, that is, secreting extracellular polysaccharidases into the surrounding medium and translocating the breakdown products of enzymes through porins, Bacteroides spp. appear first to bind the polysaccharide at the cell surface and then to transport the molecule into the …
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Molecular mechanisms of pathogenesis incited by Erwinia carotovora subsp. carotovora
… soft-rot is due to the concerted activity of extracellular enzymes. Recombinant DNA techniques were used to study the molecular basis of pathogenesis incited by Ecc. Specifically, a clone library of Ecc strain EC14 DNA in plasmid pBR322 was constructed and transformed into Escherichia coli …
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Phenotypic and genotypic characterisation of Clostridium Difficile
… investigated for the production of a range of extracellular enzymes as potential virulence factors. Genotypic characterisation was performed using a random amplification of polymorphic DNA(RAPD) PCR protocol which was fully optimised in this study, and the gold standard method, PCR ribotyping. …
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The Effects of dairy cattle antibiotics on soil microbial community cycling and antibiotic resistance
… communities by measuring the activity of extracellular enzymes associated with organic matter processing and nutrient mineralization in soils collected from dairy cattle operations across the United States. At each experimental station paired sites were identified by local managers that …
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Pengaruh filtrat bakteri endofit tanaman kentang (Solanum tuberosum L.) terhadap mortalitas larva II Globodera rostochiensis W.
… with these plants. Endophytic bacteria produce extracellular enzymes such as the chitinase, protease and cellulase which serves to degrade the cells of nematodes. In addition Endophytic bacteria also produce compounds β-endotoxin and δ-exotoksin that can infect the nervous system and …
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Effects of land management and climate change on soil microbial communities in Appalachian forest ecosystems
… resulted in elevated production of microbial extracellular enzymes, which increased rates of C and N cycling in soils following Rhododendron removal. In chapter 3, I examined soils across several historically disturbed and adjacent undisturbed reference forests and show that historical …
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