Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Extracellular DNA"”.
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Extracellular DNA-Based Trapping of Heavy Metals by Root Border Cells
… studies have revealed that, as in animals, extracellular DNA (exDNA) trapping plays a central role in defense, but the mechanism remains poorly understood. Upon immersion in water, border cells and the associated exDNA mucilage structures rapidly disperse and are primed for function in …
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Synthesis, Radiolabeling and Evaluation of a suite of Tracers with <sup>44</sup>Sc for Detecting Extracellular DNA
<p>Neutrophil extracellular traps involve the rapid translocation of DNA to the outside of the cell under certain stimuli. This structure forms a fibrous network that is able to limit the spread of pathogens and to kill microorganisms. It has also been shown to be present in various pathological …
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Bacterial transport and horizontal gene transfer in subsurface: multiple-scale experimental studies
… cells successfully take up and incorporate extracellular DNA. The extracellular DNA and the bacterial cells are both exposed to various environmental conditions that may alter natural transformation. Bacterial fate and transport in the subsurface may influence bacterial transformation with …
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Phylogenetic and physiological characterization of deep-biosphere microorganisms in El’gygytgyn Crater Lake sediments
… 3.6 Ma to 1.0 Ma (Pliocene and Pleistocene). DNA from all investigated sediments was successfully extracted and a surprisingly diverse (6,910 OTUs) and abundant microbial community in the El’gygytgyn deep sediments were revealed. The bacterial abundance (10³-10⁶ 16S rRNA copies g⁻¹ sediment) …
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Structure and Function of Bordetella Transcription Factor BpsR
… composed of polysaccharides, proteins, and extracellular DNA. They help bacteria persist, resist and evade host immune response. In Bordetella, the Bordetella polysaccharide (Bps) is a crucial polysaccharide component of a mature biofilm. Bps production is controlled by the bps locus. This …
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Applying Classical Particle Aggregation Modeling Techniques to Investigate the Heteroaggregation of Environmental Biocolloids
… primary biological particles: bacteriophages, extracellular vesicles, and extracellular DNA, each of them often existing on the nanoscale. This was done by 1) using modified Smoluchowskian aggregation parameters to predict phage-induced host lysis, 2) characterize phage-kaolinite …
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Effect of Exopolysaccharides on Pseudomonas aeruginosa Physiology
… that ionic interactions between Pel and extracellular DNA (eDNA) increase tolerance to aminoglycoside treatment. Additionally, I determine that cell-association of Psl is promoted post-translationally by the diguanylate cyclase SiaD, which stimulates initial attachment to surfaces. This …
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Effect of wastewater colloids on membrane removal of microconstituent antibiotic resistance genes
… colloids on their removal. Native and spiked extracellular vanA (vancomycin resistance) and blaTEM (β-lactam resistance) ARGs were tracked by quantitative polymerase chain reaction through a cascade of membrane filtration steps. To gain insight into potential associations occurring between …
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Role of Bacterial Lipocalin Proteins in Antimicrobial Resistance
… in patients with cystic fibrosis, produces the extracellular bacterial lipocalin protein BcnA upon exposure to<br/>sublethal concentrations of bactericidal antibiotics. BcnA captures a range of antibiotics outside bacterial cells, providing a global extracellular mechanism of antimicrobial …
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Endocytosis of therapeutic macromolecules in tumor cells - Mechanistic aspects of the proteoglycan receptor function
… that mammalian cells can internalize extracellular DNA by a pathway strictly dependent on cell surface PGs and this pathway is characterized. Secreted, positively charged, proteins and peptides including the antimicrobial peptide LL-37, are shown to facilitate the uptake process. It is …
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The role of biofilms in Campylobacter jejuni survival in the food chain
… population, surrounded by a self-produced extracellular matrix. Previous work has shown that biofilm formation by C. jejuni is increased in food chain relevant conditions, but C. jejuni biofilms have received little attention compared to other foodborne pathogens. The work presented here …
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Characterizing the Role of the Type VI Secretion System in Interbacterial Species Interactions and Pathogenesis
… including cell wall, membrane lipids, and DNA. Despite the importance of the T6SS as an interbacterial weapon, there still exists an incomplete understanding of how T6SS expressing species can co-exist and the implications of antagonistic responses in shaping polymicrobial communities. …
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The contribution of competence system to the virulence of Streptococcus pneumoniae
… genetic transformation, the only one that occurs extracellularlly is the interaction between CSP and ComD. I hypothesized that blocking CSP-ComD interaction could effectively inhibit the induction of competence system. Previous studies have shown that the variable central region of CSP determines …
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The Role of the Extracellular Matrix components of Salmonella enterica serovar Typhimurium Biofilms in Biofilm Formation and Architecture via in vitro analysis
… bacterial cells enclosed within a self-generated extracellular matrix, forming a three-dimensional structure that allows the bacteria cells to firmly attach to both living and non-living surfaces. The extracellular matrix of Salmonella enterica serovar Typhimurium (STm) biofilms is composed of …
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Identification and characterization of a novel capsule-like complex surface antigen of Francisella tularensis
… known virulence determinants, such as GroEL, DnaK, and ClpB. Upon further evaluation of the CLC, I determined that it was composed of an increase in production of outer membrane vesicles and tubules (OMV/T). These OMV/T appeared to be self-aggregating into what I visualized through TEM as the …
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Molecular Insights into the Uropathogenesis of Enterococcus faecalis and Escherichia coli in the Context of Urinary Catheterization
… that <italic>E. faecalis</italic> produces DNA-dependent biofilms, which require both SrtA and Atn for efficient attachment as well as extracellular DNA from autolytic processes during the accumulative phase for maturation and architectural stability of the biofilm under both static and …
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Human impact on the McMurdo Dry Valley soils of Antarctica: Extending the limits of DNA detection
… research quantified human impact, in the form of DNA released from humans and human-associated microbiota, across spatial and temporal scales. This project was part of the MBIE grant entitled “Evidence-based Risk Assessment of the McMurdo Dry Valley Ecosystem”. Areas of human activity, including …
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Antimicrobial resistance in soil: long-term effects on microbial communities, interactions with soil properties, and transport of antimicrobial elements
… of AMR in the form of viable ARB or ARGs in extracellular plasmids. Our results demonstrate that soil bacterial community structures were consistently altered by 3-year additions of manure from cattle administered antibiotics compared to soil amended with antibiotic-free manure. Furthermore, …
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Characterisation of the ATP-dependent ligase, Lig E: An investigation into its significance in Neisseria gonorrhoeae
… rates due to the bacterium’s ability to take up DNA from its environment without regulation. With approximately 106 million new cases annually, thorough investigations into novel gonococcal pathways that can be targeted to limit its survival and spread are crucial. One possible target is the …
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