Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 57 for “"Experimental Pathology"”.
-
Role of DARPP-32 in Breast Cancer Cell Signalling and Migration
Breast cancer is the most common form of cancer in women. While the prognosis for breast cancer patients without local or distal dissemination is relatively favorable, the prognosis is considerably worse once distal metastasis has been established. It is therefore imperative to identify molecular …
-
Wnt-5a signaling in breast cancer metastasis
Breast cancer is the most common form of malignancy affecting women in the Western world. Today, anticancer treatment can control primary breast tumors, but treatment and prevention of metastatic disease still represents a major challenge in the management of breast cancer patients. It has been …
-
Dysregulated TFH cell and germinal center activation in mice with severe Orientia tsutsugamushi infection
Scrub typhus is a severely understudied tropical disease caused by Orientia tsutsugamushi. It is endemic in, but not limited to, the Asia-Pacific region, with about one million cases reported every year. Its high case fatality rates and impact during WWII and the Vietnam War justify vaccine …
-
Investigations into endothelial cell permeability and adherens junction disruption induced by Junín virus infection.
… distribution abnormalities. Clinical, as well as experimental studies, have implicated involvement of the endothelium in pathogenesis of AHF, although the role it may play is poorly understood. Junín virus has been shown to produce productive infection of endothelial cells in vitro with no visible …
-
The development of a reverse genetics system for Machupo virus
The etiologic agent of Bolivian hemorrhagic fever (BHF), Machupo virus (MACV), is a highly lethal viral pathogen with no approved vaccine or therapeutic to limit infection or outbreaks. Following implementation of rodent population controls after the initial outbreak, no cases of BHF were reported …
-
Impact of West Nile Virus Envelope Domain III Surface Loop Residues on Antigenicity and Virulence
West Nile virus (WNV) is an encephalitic flavivirus that is maintained worldwide in a transmission cycle between birds and mosquitoes. Domain III of its envelope protein is the target of highly specific and potently neutralizing antibodies, and is thought to bind the host cell receptors. To …
-
Using Altered Fidelity Viruses to Study Viral Attenuation and Population Genetics During Vertebrate Host Infection
The requirement of arthropod and vertebrate hosts for the host life cycle of an arbovirus constrains its evolution. For the viral population to survive in two hosts, the ability to produce genetic diversity within the population is essential. RNA viruses contain an RNA dependent RNA polymerase …
-
Ehrlichia chaffeensis Type 1 Secreted Effector TRP47 is a Novel SUMOylated Nucleomodulin
Ehrlichia chaffeensis secretes immunodominant and immunoprotective protein effectors into the host cell using a type I secretion system. The best-characterized of these effectors are the tandem repeat proteins (TRPs). In this study, E. chaffeensis TRP47 was identified as a target for host cell …
-
Phenotypic Characterization of Mutations Made to the Envelope Protein of West Nile Virus
… with host cells in vitro and in vivo. Experimental results demonstrated that G331A mutation induces significantly attenuated neurovirulent and neuroinvasive phenotypes, and that the mutation impacted multiple phenotypes associated with attenuation of neurotropic flaviviruses. …
-
Description of interactions of Ebola virus with a putative reservoir species using next-generation sequencing
… I also describe the evolution of EBOV in an experimental passage series in bat and human cells. This work led to the discovery of a potential role for host RNA editing enzymes in the evolution of EBOV in bats, and identified loci within the viral genome that appear to be associated with …
-
Production and Testing of a Novel Live-Attenuated Chikungunya Vaccine
The following work was focused on production and testing of a novel live-attenuated chikungunya virus (CHIKV) vaccine. To this end, multiple strategies were adapted from the IRES-based vaccine strategy first employed with VEEV vaccine strain, TC-83. The first strategy, CHIKV/IRESv1, was highly …
-
Mechanism of transmissible gastroenteritis virus nsp1-mediated translation inhibition
The non-structural protein 1 (nsp1) proteins of several coronaviruses share a common property to inhibit host gene expression, but use different mechanisms to exert this function. Unlike the nsp1 protein of SARS coronavirus, a group 2b betacoronavirus, the nsp1 protein of transmissible …
-
The Activation of the Inflammasome during Rickettsial Infection
Rickettsiae are Gram-negative, obligately intracellular bacteria that infect endothelial cells and macrophages. The molecular mechanisms involved in interaction of rickettsiae with macrophages, however, remain poorly understood. In this dissertation, I have investigated the canonical and …
-
Neutrophil Regulation of Colitis
The delicate intestinal homeostasis is regulated by interactions between microbiota, intestinal epithelial cells (IECs), and the intestinal immune system. As a crucial component of the innate immune system, neutrophils are the first responders to sites of inflammation when the intestinal epithelial …
-
The introduction of incompatibility between Junin virus Z protein and other viral proteins results in impaired replication and attenuation
… resulted in attenuation of the virus and a pathology profile similar to rCan. In addition, it was narrowed down to a single mutation (V64G) within the Z that conferred the attenuation when paired with Rom LP. The LP is comprised of four domains. We generated chimeric LPs representing …
-
Eukaryotic protein mimicry as an infection strategy for Ehrlichia chaffeensis
Ehrlichia chaffeensis (E. ch.) expresses the TRP120 multifunctional effector on the surface of infectious dense-cored ehrlichiae which is known to play a role in phagocytic entry, but a cognate host receptor has not been identified. We recently reported that E. ch. activates canonical Wnt signaling …
-
Host-Dependent Expression, Transcriptional Regulation and Protection of Ehrlichia chaffeensis Tandem Repeat Proteins
Ehrlichia chaffeensis is an obligately intracellular bacterium that is the causative agent of human monocytotropic ehrlichiosis, an emerging life-threatening zoonosis. E. chaffeensis is transmitted by the lone star tick, Amblyomma americanum, and replicates in mononuclear phagocytes in mammalian …
-
The Balance of Interferon-γ and Interleukin-10 during Influenza A Virus Infection Complicated by Methicillin-Resistant Staphylococcus aureus Superinfection
… phagocytes in shifting the balance between immunopathology and protective immunity. By omitting antibiotic therapy from the superinfection model, we show that IL-10 also impairs bacterial clearance, specifically when the IL-10Rα gene is ablated in interferon-I-responsive monocytes. Collectively, …
-
HIJACKING OF CELLULAR PATHWAYS BY NOVEL TICK-BORNE PHLEBOVIRUS
Severe Fever with Thrombocytopenia Syndrome (SFTS) virus is an emerging tick-borne Phlebovirus isolated from patients presenting with hemorrhagic manifestations. Case fatality rates of 12-50% have been reported. Limited information regarding SFTS virus pathogenesis is known. Inhibition of …
Page 1 of 3