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Showing 1 to 20 of 32 for “"Expansion Microscopy"”.
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Expansion Microscopy of Cells in Suspension
Expansion microscopy is a laboratory technique that enables nanoscale imaging of biological samples with conventional light microscopes. While expansion microscopy has traditionally been applied to specimens consisting of tissue and adherent cell culture, it has not been optimized for specimens …
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Enabling scalable multicolor connectomics through expansion microscopy
… such resolution for some time thanks to electron microscopy (EM), the analysis of the resulting data has proven to be a very challenging task, mainly because it relies only on membrane contrast to differentiate cells. Expansion microscopy (ExM) offers an exciting alternative to EM by expanding …
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Expansion microscopy : scalable and multiplexed nanoscale imaging
Microscopy has facilitated the discovery of many biological insights by optically magnifying small structures in cells and tissues. However, the resolution of optical microscopy is limited by the diffraction of light to ~200-300 nm, comparable or larger to the size of many subcellular structures. …
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Expansion microscopy : improving imaging through uniform tissue expansion
Until the past decade, optical microscopy of biological specimens was strongly limited by diffraction and scattering, affecting imaging resolution and depth, respectively. Now, numerous methods are available to overcome each of these limitations, but sub-diffraction limited resolution imaging over …
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Quantitative nanometer-scale thermal metrology using scanning joule expansion microscopy
Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by William Ingram (wingram2@illinois.edu) on 2011-05-25T14:41:49Z Item is restricted until 2013-05-25T14:41:28Z
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Toward Ultra-Resolution Biomolecular Mapping in Cells with Expansion Microscopy
… resolution in biomolecular imaging is essential. Expansion microscopy (ExM)1, a new kind of super-resolution microscopy, enables this by physically enlarging preserved biological specimens. Thus allows the investigation of structure-function relationships at nanoscale resolution using conventional …
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Nanoscale biomolecular mapping in cells and tissues with expansion microscopy
… this crucial ability remains an unmet need. Expansion Microscopy (ExM) enables the 3D, nanoscale imaging of biological structures by physically magnifying cells and tissues. Specimens, embedded in a swellable hydrogel, undergo uniform expansion as covalently anchored labels and tags are …
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Expansion Microscopy of Extracellular Space for Light Microscopy-Based Connectomic Analysis
… we present an exploratory methodology, termed expansion microscopy of extracellular space (ExECS), designed to enhance the visualization of the extracellular space (ECS) within aldehyde-fixed tissue. This technique leverages the principles of expansion microscopy (ExM), a method that …
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Expansion microscopy of C. elegans : nanoscale imaging of biomolecules throughout an entire organism
Expansion microscopy (ExM) enables 3-D, nanoscale-precise imaging of biological specimens by isotropic swelling of hydrogel-embedded, chemically processed tissue. Such capability raises the question of whether nanoscale mapping of biomolecules could be performed in an entire organism, which would …
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Towards optical connectomics : feasibility of 3D reconstruction of neural morphology using expansion microscopy and in situ molecular barcoding
… and functions of neural circuits. Optical microscopy has been a key technology for mapping brain circuits throughout the history of neuroscience due to its low cost, wide usage in biological sciences and ability to read out information-rich molecular signals. However, conventional optical …
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Expansion microscopy to visualize cell-cycle events in the abbreviated cell cycle in <i>Caenorhabditis elegans</i> (roundworm) germline stem cells
… cell cycle, we optimized a protocol for expansion microscopy in stem cells of the reproductive system of the model organism C. elegans. Optimizations included development of new mounting techniques and modifications to DNA staining methods. These optimizations produced brighter samples …
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Scalable Chemical Tool for Analyzing Brain: Electron Microscope-Like Images with Fluorescent Microscope
… ultrastructure and molecular features. Electron microscopy (EM) has greatly advanced our fundamental understanding of neurobiology by revealing ultrastructural features through dense labeling of membranous structures while fluorescence microscopy (FM) allowed neuroscientists to identify and study …
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Super-resolved volumetric optical imaging of virus-infected cells
… disruptive pandemics. This thesis shows how expansion microscopy is an excellent tool to achieve super-resolution when imaging virus-infected samples using optical microscopy. Expansion microscopy is a recently conceived sample preparation technique that physically expands fixed specimens …
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Towards sequencing by synthesis In Situ
… sample engineering, including physical expansion of tissue (i.e. Expansion Microscopy), will radically empower this technology (ExSEQ). However, in situ sequencing is technically difficult to implement, requiring an investigator to be familiar with a wide variety of techniques in …
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Tools for connectomics in C. elegans
… for the development of such a framework. The expansion microscopy tissue preparation platform provided the basis for the set of experiments described within, in which strategies for molecular annotation of C. elegans and the subsequent protocols for readout are examined.
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Modeling of Joule heating and thermoelectric transport in thin film silicon for SJEM measurement
… data acquisition technique of Scanning Joule expansion microscopy (SJEM). Time varying heat equations and Maxwell’s equations are solved in frequency domain and applied to commercial finite element software, COMSOL. Chapter 1 introduces various techniques to obtain nanoscale temperature …
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Technological Innovation and Integration of Whole Brain Imaging, Olfactory Stimulation, and Correlative Microscopy in Larval Zebrafish
… brain. In this work we show novel light field microscopy algorithms that allow us to reconstruct 3D images from 2D micrographs with improved resolution to enable high-frame-rate recordings of whole-brain neural activity. We describe the designing and building of the first known system for …
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Advanced purification of aligned arrays of single-walled carbon nanotubes
… develop a nanoscale thermometry-scanning Joule expansion microscopy (SJEM) for quantitative assessments of the low temperature nature associated with this process. Measurements combined with simulations fully reveal the essential physics of the thermocapillary flows. We also introduce a simple …
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Highly multiplexed molecular mapping of biological samples via integrated experimental and computational technologies
… price of decreased spatial information. Electron microscopy, with its nanometer spatial resolution and excellent ultrastructure preservation, yet essentially no molecular identification, is the most obvious example of this dichotomy that pervades all imaging paradigms. The Boyden Lab has …
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Toward the Understanding of Brain’s Molecular Language
… We propose that recent technological advances in expansion microscopy, molecular anchoring, machine learning-enabled protein detection, and cryo-fixation now make it possible to map molecular networks in their native context. This integrative approach promises to illuminate the molecular …
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