Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Exonucleases"”.
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The role of TREX1 and TREX2 in cellular responses to cisplatin
… cancer cells identified the 3’ to 5’ exonucleases TREX1 and TREX2 as significantly upregulated DDR genes. Increased expression was confirmed at the protein level in two distinct cancer cell lines. Individual depletion of TREX1 and TREX2 had no effect on cell viability under basal …
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Structural and Biochemical Investigation of the Pan2-Pan3 Deadenylase Complex
… preference, it was unknown how the conserved exonucleases Pan2 and Caf1 recognise poly(A). In Chapter 2, structural and biochemical studies reveal that these DEDD-family deadenylases recognise the intrinsic stacked, helical structure of single-stranded poly(A) RNA. Recognition of this …
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Investigation of a kalilo plasmid in Louisiana Neurospora tetrasperma
… digestion of the kalilo' plasmid with 5' and 3' exonucleases showed a 5' resistance to enzyme digestion and a 3' sensitivity to digestion. This suggested that kalilo' may have a protein bound to its 5' termini, as is the case with kalilo. Biological properties of the Louisiana plasmid were also …
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Laboratory evolution and natural transformation in Acinetobacter baylyi ADP1
… DNA to the ends of fragments to buffer against exonucleases and altering the abundance of a single-stranded DNA protecting protein, did not. Chapter 4 describes an effort to better characterize the machinery required for competence in ADP1. We coupled transformation-mediated selection of an ADP1 …
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REGULATION OF EUKARYOTIC GENE EXPRESSION BY mRNA CAP BINDING COMPLEX AND CAPPING MACHINERY
… cap-binding complex (CBC). CBC shields mRNA from exonucleases as well as regulates downstream post-transcriptional events, translational initiation and nonsense mediated mRNA decay (NMD). Any misregulation during capping or in the binding of CBC can lead a number of diseases/disorders. Thus, the …
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Isolation of antimutator yeast strains: an exploration of DNA replication fidelity
… enzymes with intrinsic proofreading exonucleases that remove errors during DNA synthesis. Errors that elude proofreading are extended into duplex DNA and excised by the mismatch repair (MMR) system. Cells that lack Pol proofreading or MMR exhibit a mutator phenotype, manifested as a …
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The TREX1 3' Exonuclease and Autoimmune Disease: Structural and Biochemical Analysis of Disease Mutants Involved in Autoimmune Dysfunction
… TREX1 protein is a member of the DnaQ family of exonucleases and catalyzes the major 3′ exonuclease activity detected in mammalian cell extracts. Mutations within the Trex1 gene are the underlying cause of multiple autoimmune diseases including Aicardi-Goutières syndrome (AGS), familial chilblain …
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A Census of Human RNA-Binding Proteins and Characterization of the DEDDh RNA Exonuclease NEF-sp in the 3' End Maturation of 28S Ribosomal RNA
… mediated by the regulated expression of RNA exonucleases.</p>
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The Mechanism of RNA Interference in Neurospora
In the canonical RNA interference (RNAi) pathway, small-interfering RNA (siRNA) duplexes generated by Dicer are incorporated into the RNA-induced-silencing complex (RISC), and subsequently converted to single-stranded siRNA. Generation of single stranded siRNA is a pre-requisite for recognition and …
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Functional Roles of Nucleases in DNA Metabolism and Genome Stability
… of bi-directional single-strand DNA specific exonucleases that all contain an iron-sulfur cluster as a structural motif and all have various roles in DNA metabolism. In the Saccharomycetales order that includes the budding yeast, S. cerevisiae, Exo5 is a mitochondrial protein that is essential …