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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 144 for “"Exocytosis"”.
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Exploring Synaptic Vesicle Exocytosis
… vesicles, exercises a negative control on exocytosis. Reducing the levels of this protein, without eliminating it completely from a synapse, leads to increases in Pv, but does not affect n. In addition to measuring synaptic properties of individual neurons, our methods can be used to study …
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Calcium Triggered Synaptic Vesicle Exocytosis
Neurotransmitter release is triggered by the action potential induced influx of Ca 2+ into nerve terminals. One of the central questions in neuroscience is how Ca 2+ promotes synaptic vesicles from rest to fusion leading to release of neurotransmitters. In this thesis, I first addressed if …
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Regulation of Exocytosis by Syntaxin 4-Munc18c Complexes
… in regulating Syn4-Munc18c interaction and exocytosis in beta cells and peripheral tissues, manipulations of this complex may have therapeutic potential as a strategy to treat Type 2 diabetes.
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Munc18 function in large dense-core vesicle exocytosis
… and hormon release is based on Ca2+ dependent exocytosis. The sequential steps of exocytosis are executed by proteins and protein complexes. Formation of the SNARE complex is essential in later steps of exocytosis. The Sec1/Munc18 proteins are also essential in exocytosis and other membrane …
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Molecular Determinants of Synaptic Vesicle Exocytosis and Endocytosis Coupling
… availability of synaptic vesicles for subsequent exocytosis. The coupling of exocytosis and endocytosis allows for continued rapid synaptic transmission; however, the molecular mechanisms of this process are not well understood. This coupling is assumed to be Ca2+-dependent but the exact role of …
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Characterization of Synaptotagmin Function In Calcium Dependent Neuronal Exocytosis
Es wird vermutet, dass das Protein Synaptotagmin 1, das in der Membrane des synaptischen Vesikels sitzt, der Kalziumsensor in der neuronalen Synapse ist. Synaptotagmin 1 hat zwei sogenannte C2 Domänen, die kalziumabhängig Membranen binden. Es ist gezeigt worden, dass die Deletion von Synaptotagmin …
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SNARE-mediated exocytosis of MUC2 from intestinal goblet cells
… the SNARE complex in goblet cells mediates mucin exocytosis and degradation of these regulatory pathways exacerbates pathogenesis of the intestinal pathogen Entamoeba histolytica. The SNARE complex is the classical exocytosis machinery as the effector mechanism governing mucin secretion. In this …
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The Role of [beta]2-Syntrophin Phosphorylation in Secretory Granule Exocytosis
… actin cytoskeleton modulates the mobility and exocytosis of SGs,yet the mechanisms anchoring SGs to the cytoskeleton is not completely understood.It has been shown by Ort et al.(2000,2001) that the cytoplasmic tail of an intrinsic membrane protein of the SGs named ICA512/IA-2 binds the PDZ …
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Identifying the Role of Phospholipase D1 and Phosphatidic Acid in Exocytosis
<p>Exocytosis is an essential process for intercellular communication in eukaryotic cells. This process involves significant changes in membrane curvature, and lipids and curvature-sensing proteins can assist these processes. One protein in particular, phospholipase D1 (PLD1), and its product, the …
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Expression and Function of Complexin 1 in Mammalian Sperm Acrosomal Exocytosis
… for a wide variety of cellular events including exocytosis. Exocytosis is regulated by the SNARE complex, whose core includes three proteins. Complexins 1 and 2 are highly related cytosolic proteins that bind tightly to the assembled SNARE complex and promote neuronal exocytosis. Studies of …
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Unraveling the Molecular Mechanisms of Munc18 and Munc13 in Mast Cell Exocytosis
Exocytosis is a fundamental cellular process in which contents stored in vesicles are released out of cells via fusion of membranes. Exocytosis of secretory granules from immune cells such as cytotoxic T-lymphocytes, natural killer cells and mast cells play crucial physiological roles in protecting …
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Measurement and Analysis of Calcium-Dependent Exocytosis in Giant Excised Membrane Patches
Ca2+-dependent exocytosis was studied in both excised and whole-cell patch clamp with emphasis on the rat secretory cell line, RBL. Capacitance and amperometric recordings show that secretory granules (SGs) containing serotonin are mostly lost from excised patches. Small vesicles that are retained …
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Characterization of the FXYD Protein Family in the Regulation of Insulin Exocytosis
The file named "hayslori.pdf" is the primary dissertation file. All other files ("*.mov") are supplemental files and may be viewed individually.
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Exocytosis in Type 2 Diabetes- Functional and genetic studies of hormone secretion
… Several proteins are crucial in maintaining exocytosis of the hormone-containing granules such as Syntaxin1A (Stx1A), SNAP25, Munc-18 (Stxbp1) and the family of Synaptotagmins (Syts). The aim of this thesis has been to investigate functional and genetic events that participate in the …
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Regulation of Weibel-Palade Body Exocytosis by alpha-Synuclein in Endothelial Cells
… cytokines. This study explored whether the exocytosis of WPB is regulated by a-synuclein. Results showed that PMA-, thrombin- or forskolin-induced VWF release or translocation of P-selectin from Human Umbilical Vein Endothelial Cells (HUVECs) were inhibited by overexpression of a- and …
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Determining the Roles of Q-SNAREs in Mast Cell Tumor Necrosis Factor Exocytosis
… of the mechanisms that regulate TNF exocytosis in mast cells remains woefully inadequate, and expanding this knowledge would undoubtedly aid in the development of therapeutics to specifically target mast cell TNF secretion. To explore the roles to SNAREs in TNF release in mast cells, …
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Frequency Selectivity of Synaptic Exocytosis in Hair Cells of the Bullfrog's Amphibian Papilla
… cells display frequency selectivity in synaptic exocytosis over the range of frequencies sensed by the organ. Moreover, this tuning varies from cell to cell in accordance with the cells' tonotopic position. Using confocal imaging, we determined that hair cells tuned to high frequencies have a …
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Nature of Large Vesicle Exocytosis in Pancreatic β-cells: Release of ATP and GABA
… of GABA release events were attributable to the exocytosis of large dense core vesicles in these cells and its selective release was regulated by the size of the fusion pore. The combination of the cell capacitance measurements, the electrical measurements of ATP release and amperometric …
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The regulation of luteinizing hormone exocytosis in α-toxin permeabilized sheep anterior pituitary cells
Although exocytosis is the major mechanism by which cells secrete products into their environment, little is known about the mechanism of this fundamental process. Previous studies on the regulation of luteinizing hormone (LH) exocytosis have used intact cells exclusively. It is not possible, …
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Competition Between Synaptotagmin 1 and Complexin for SNARE Complex Binding, Controls Fast Synaptic Vesicle Exocytosis
Calcium binding to synaptotagmin 1 triggers fast exocytosis of synaptic vesicles that were primed for release by SNARE complex assembly. Besides synaptotagmin 1, fast Ca2+- triggered exocytosis requires complexins. Synaptotagmin 1 and complexins both bind to assembled SNARE complexes, but it is …
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