Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Ex vivo expansion"”.
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Lung Progenitor Cell Transplantation for Pulmonary Fibrosis and Ex Vivo Expansion of Patch-Forming EpCAM⁺CD31⁺ Cells
… CD45- subpopulation. More recent FACS sorting experiments revealed that this curative potential resides within the small CD45-CD326+CD31+ subpopulation. However, these studies also indicated that in this mouse model successful transplantation requires vacating the lung niches in recipient mice …
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Characterization and Optimization of Antigen-Specific T Cell Responses During Ex Vivo Expansion of Melanoma Tumor-Infiltrating Lymphocytes
… (pre-REP) of tumor-infiltrating lymphocyte (TIL) expansion ex vivo from tumor isolates followed by a second phase, “rapid expansion protocol” (REP) generating the billions of cells used as the TIL infusion product. The main question addressed in this thesis was how the currently used REP affected …
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Hematopoietic Stem Cell Threshold Sensing Controls Regulatory Pathways Facilitating Clinically Relevant Ex Vivo Expansion for Stem Cell Transplantation
<p>The ex vivo expansion of hematopoietic stem cells (HSCs) for transplantation has threefold importance: 1.) First, because of the rarity of stem cells there often isn’t a sufficient supply obtainable from common sources for larger children and adults. 2.) Secondly, patient morbidity and time to …
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Eviscerated corneas as tissue source for ex vivo expansion of limbal epithelial cells on platelet-rich plasma gels
… To assess if corneal epithelium can be cultured ex-vivo from corneas eviscerated due to irretrievable trauma, according to a cell culture method which made use of autologous platelet-rich plasma (A-PRP) as culture substrate. To compare corneal epithelium cultured ex vivo from corneas eviscerated …
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Investigation of a suppression of asymmetric cell kinetics (SACK) approach for ex vivo expansion of human hematopoietic stem cells
Ex vivo expansion of hematopoietic stem cells (HSCs) is a long-standing challenge faced by both researchers and clinicians. To date, no robust, efficient method for the pure, ex vivo expansion of human HSCs has been demonstrated. Previous methods primarily induced the expansion of committed …
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The roll of integrins in hematopoiesis
… factor in both their study and clinical use. ex vivo expansion of these cells is therefore a necessary step to maximizing their potential. In this thesis I explore the concept that signals from the extracellular matrix can direct differentiation, survival and self-renewal decisions in …
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Identification of Novel Drivers of Haematopoietic Stem Cell Fate Using Low Cell Number Proteomics and Single Cell Profiling
… and to help inform novel strategies for ex vivo expansion for gene therapy applications. To date, advances in functional and molecular single cell technologies have provided unprecedented resolution of heterogeneous HSC populations and their transcriptional landscapes. In contrast, the …
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Insulin-Like Growth Factor-Binding Protein 2 Supports Hematopoietic Stem Cell Expansion: From In Vitro to In Vivo
… by the numbers of HSCs, and robust methods to expand HSCs ex vivo are needed. We previously showed that angiopoietin-like proteins (Angptls), a group of growth factors isolated from a fetal liver HSC supportive cell population, improved ex vivo expansion of HSCs. Here we demonstrate that …
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Engineering Liver and Cardiac Models for Drug Development and Regenerative Medicine
… mimicking the bone marrow microenvironment for expansion of hematopoietic stem cells (HSCs) are increasingly studied for cell therapies, but long-term ex vivo expansion of HSCs using the factors identified during these studies has not been achieved. Finally, induced pluripotent stem cell-derived …
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Stem cell matrix and cartilage regeneration
… is cell senescence, which is due mainly to extensive ex vivo passaging and elderly donors. A reconstructed ex vivo microenvironment that can maintain or enhance stemness is urgently needed for facilitating large-scale tissue engineering. To facilitate ex vivo expansion, the conventional …
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Clinical Applications of Stem Cells in Ocular Surface Reconstruction.
… thesis is to improve current techniques for the ex vivo expansion of epithelial and endothelial cells on amniotic membrane for the treatment of ocular disorders. This thesis is primarily a microscope-based study and documents the findings of several investigations designed to refine tissue …
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Membrane Bound Il21 Promotes Natural Killer Cell Expansion Through Mir 124-3P Mediated Regulation
… host disease (GvHD), or require prior antigen exposure to exert anti-tumor activity, hence are an attractive choice for immunotherapy applications. Owing to small numbers of NK cells in peripheral blood (1-32%, with a 6% median), ex vivo expansion of NK cells is critical for NK cell adoptive …
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Engineering B Cells For Use In Immunotherapy and Cancer
… and transforming growth factor-beta (TGF-b). Ex vivo expansion of B cells has been challenging due to their tendency to undergo apoptosis in culture. Thus, creating a successful expansion protocol with exceptional viability will open the door for B cells to be used directly for adoptive …
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Improving Leukocyte Recovery Through Physiologically Relevant Oxygen Expansion after Hematopoietic Stem Cell Transplantation
… disease. Few effective clinical therapies exist to enhance lymphocyte recovery, indicating a clear unmet need. Ex vivo expansion of umbilical cord blood (CB) cells is an FDA approved therapy to increase recovery of critical hematopoietic cells such as neutrophils and platelets after HCT; …
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Development of a novel approach to expand umbilical cord blood-derived haematopoietic stem/progenitor cells ex vivo for clinical transplantation
… barrier, many strategies have been tested to expand HSPC numbers ex vivo. The aims of this thesis were to define the phenotypic, functional and transcriptional changes that occur during ex vivo expansion of UCB HSPC and investigate how activation of the Wingless (Wnt) pathway via GSK-3 …
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Role of Polycomb Group Protein Mel18 in Hematopoietic Stem Cell Maintenance
… studies indicate that Polycomb group proteins exist in at least two protein complexes, Polycomb repressive complex 2 (PRC2) and Polycomb repressive complex 1 (PRC1). PRC2 complex deposits the mono-, di-, and tri- methylation on histone 3 lysine 27, whereas PRC1 introduces mono-ubiquitination on …
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The Role of Hyaluronan in the Ocular Surface
Purpose: The extracellular matrix (ECM) has a vital role in all tissues of the body, including maintaining ocular surface homeostasis. Hyaluronan (HA) is found in the extracellular matrices of the corneal limbus and meibomian gland (MG). This dissertation evaluated the role of hyaluronan in ocular …
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Autologous Proliferative and Immunobiological Modelling of Patient-Derived Clear Cell Renal Cell Carcinoma and Kidney Organoids
… immune modelling of ccRCC in vitro using expanded patient-derived ccRCC, kidney and peripheral blood tissues. However, patient-derived ccRCC organoids (PDO-ccRCC) proliferate poorly ex vivo and the consequences of expansion on healthy kidney organoid controls (PDO-KO) and autologous T cell …
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Rational design to control multipotent stromal cell migration for applications in bone tissue engineering and injury repair
… is the scarcity of MSC numbers isolated from in vivo, suggesting a role for biomimetic scaffolds in the cells' ex vivo expansion before reintegration into target tissue. Toward this end, immobilized epidermal growth factor (tEGF) has recently been found to promote MSC survival and proliferation …
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Transcription factor modulation in T-cells to improve cancer immunotherapy
Adoptive transfer of T-cells expressing chimeric antigen receptors (CAR-T) effectively treats refractory hematologic malignancies in a subset of patients, but can be limited by the inability of ex vivo culture systems to expand T-cells sufficiently for effective dosing while producing highly active …
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