Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Evolutionary engineering"”.
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Design Optimization of a Composite Wing Box for High-Altitude Long-Endurance Aircraft
… Element Analysis (FEA), and the Hierarchical Evolutionary Engineering Design System (HEEDS) MDO software to define a locus of acceptable, optimized wing box design definitions.</p>
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Production of 1-butanol and butanol isomers by metabolically engineered Clostridium beijerinckii and Saccharomyces cerevisiae
… chemicals from microorganisms through metabolic engineering, systems biology, and synthetic biology approaches. Biobutanol is an attractive renewable biofuel and intermediate chemical that has been produced from the ABE (acetone-butanol-ethanol) fermentation by solventogenic clostridia such as …
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Metabolic engineering of yeast for xylose uptake and fermentation
… Rational and combinatorial metabolic engineering approaches coupled with transcriptomic and proteomic studies have been extensively exploited on the yeast Saccharomyces cerevisiae for improved xylose utilization. However, the resulting strains remain inapplicable for industrial …
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Stress resistance during the lag phase of wine fermentation and development of optimized yeasts
… we developed two parallel approaches. Using an evolutionary engineering approach where selective pressures typically present in lag phase were applied, we obtained evolved strains with a shorter lag phase in winemaking conditions. Whole genome sequencing allowed to identify several de novo …
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Harnessing mutagenic homologous recombination for in vivo targeted mutagenesis by TaGTEAM
A major hurdle to evolutionary engineering approaches for multigenic phenotypes is the ability to simultaneously modify multiple genes rapidly and selectively. Here, we describe a method for in vivo targeted mutagenesis in yeast, TArgeting Glycosylases To Embedded Arrays for Mutagenesis (TaGTEAM). …
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The exploitation of municipal solid waste (MSW) and related waste paper streams in the production of bioalcohol
… strains able to endure temperatures up to 40°C. Evolutionary engineering may improve tolerances to temperatures nearer to enzyme optimums (50°C). Some previously unused strains exhibited superior growth to referenced industrial strains. The above findings were integrated into a process design …
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Optimal yeast strains for the production of fuels and chemicals from lignocellulosic biomass
… during the last two decades, a method for engineering efficient xylose-fermenting strains has not been finalized yet. In this thesis study, by elucidating fundamental bottlenecks impeding the xylose metabolism, I identified and implemented powerful genetic perturbation targets for …
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Engineering yeast to synthesize high-value natural products from plant cell wall
… metabolism was improved through rational and evolutionary engineering. The evolved strain with enhanced xylodextrin consumption was further engineered for β-carotene production from Miscanthus autohydrolysates prepared by liquid hot water pretreatment. The engineered yeast strains and …
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Engineering geobacillus thermoglucosidasius for efficient production of fuels and chemicals
… for improved ethanol production by employing an evolutionary engineering strategy. We eliminated lactate and formate formation in both of the strains to divert carbon source to ethanol production. However, strains were unable to grow under microaerobic conditions. Growth of 95A1 was then …
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Systematic and combinatorial approaches for metabolic engineering of optimal yeast strains to produce fuels and chemicals
… goal of my thesis study is to use a metabolic engineering approach for developing optimal yeast cell factories capable of efficiently fermenting various sugars abundant in renewable biomass. The research has broad applications for sustainable biological production of value-added fuels and …