Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 1140 for “"Eukaryotic"”.
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Eukaryotic Transcriptional Activation Mechanism: Protein-Protein Interactions
<p>Various in vivo and in vitro assays have been employed to analyze how activators communicate with the general transcription machinery to stimulate transcription. As a first step, I analyzed the function of distinct kinds of activation domains in yeast and human. The results showed that the …
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Studies on Prokaryotic and Eukaryotic Polynucleotide Ligases
Made available in DSpace on 2014-12-14T07:14:45Z (GMT). No. of bitstreams: 1 7714926.pdf: 4224938 bytes, checksum: 8727bde8d854483297215c784ed8faf6 (MD5) Previous issue date: 1977
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Statistical mechanical modeling of eukaryotic gene regulation
Gene expression patterns are regulated by gene regulatory networks. Central to transcriptional regulation of gene expression is the regulation of the quantities of transcription factors (TFs) bound to genomic regulatory sequences. This thesis work is built on statistical mechanics to study the …
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Single-molecule studies of eukaryotic helicase loading
… viability of their progeny. The first step in eukaryotic DNA replication initiation is the loading of the heterohexameric Mcm2-7 helicase. In eukaryotes helicase loading is tightly regulated to ensure that this event only occurs during the GI phase of the cell cycle. Because helicase activation …
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Bioenergetics and metabolism of eukaryotic cell proliferation
… biomass accumulation and proliferation in eukaryotic cells. We first determined that the underlying mechanism behind why rapidly proliferating cells preferentially ferment the terminal glycolytic product pyruvate is due to an intrinsic deficiency of respiration to regenerate electron …
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On the Diversity of Eukaryotic Transcription Factor Specificities
… the degree of motif conservation between eukaryotic species at varying evolutionary distances using Similarity Regression (SR), which is an improved method to classify pairs of TFs as having similar or dissimilar motifs on the basis of their DNA-binding domain (DBD) sequences. Within major …
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Structure and Mechanism of a Eukaryotic FMN Adenylyltransferase
… By sequence comparison, bacterial and eukaryotic FMNAT enzymes belong to two different protein superfamilies and apparently utilize different set of active site residues to accomplish the same chemistry. Extensive biochemical studies from endogenous bacterial and mammalian FMNATs using …
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Probabilistic Modelling of Replication Fidelity in Eukaryotic Genomes
Eukaryotic DNA replication is composed of a complex array of molecular biological activities compounded by the pressure for faithful replication in order to maintain genetic and genomic integrity. The constraints governing DNA replication biology is of fundamental importance to understand the …
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Gene switches and their applications in eukaryotic systems
… explore some applications of gene switches in eukaryotic systems. First, we report the development of a gene switch that is able to regulate the endogenous VEGF-A expression in mammalian cell. The gene switch is specifically and reversibly controlled by 4,4’-dyhydroxybenzil, a small molecule, …
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Structural and mechanistic studies on eukaryotic UDP-galactopyranose mutases
… and L. major. The mechamism and structure these eukaryotic UGMs were examined by steady-state kinetics, rapid-reaction kinetics, trapping of reaction intermediates, fluorescence anisotropy, and X-ray crystallography. The mechanism first involves reduction of the required flavin by NADPH, followed …
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A eukaryotic platform for the enhancement of Cas nucleases
… only in bacterial cells. Here we develop a fully eukaryotic platform to evolve inefficient Cas proteins toward highly active nucleases. A first step of directed evolution is performed in yeast to isolate enhanced variants by auxotrophic selection; a further screening of the mutants directly in …
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REGULATION OF EUKARYOTIC TRANSCRIPTIONAL ELONGATION AND ASSOCIATED DNA REPAIR
Transcriptional elongation is a crucial step in eukaryotic gene regulation whose mis-regulation leads to cellular pathologies. This makes it quite imperative to aim for a better understanding of the processes regulating transcriptional elongation. An important process promoting the association of …
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Mechanical Manipulation of Eukaryotic Chromatin by DNA-Binding Proteins
<p>The eukaryotic genome is organized in many length scales, reflecting the intricacy associated with evolution of complex biological processes. This organization serves to exert spatiotemporal control of many DNA-transacting processes such as gene expression. Despite emerging progress, the …
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"Effects of nonthermal plasma on prokaryotic and eukaryotic cells"
… and stimulatory and proliferative effects in eukaryotic cells were determined to be dose-dependent phenomena.
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Computational analysis of eukaryotic metatranscriptomes from diverse marine environments
… thesis we present the computational analysis of eukaryotic phytoplankton metatranscriptome data sampled from representative marine environments; the simulation of metatranscriptome data for benchmarking computational tools; and analysis carried out on a newly sequenced eukaryotic phytoplankton …
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Multiplex genome editing and gene regulation in eukaryotic cells
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2019-05-01
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Cross-regulation and interaction between eukaryotic gene regulatory processes
… at many sequential steps. We studied several eukaryotic gene regulatory mechanisms with an emphasis on understanding the interplay between regulatory processes on a genome-wide scale. Gene splicing involves the joining of exonic RNA stretches from within a precursor messenger RNA (mRNA). …
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